In:
Proceedings of the National Academy of Sciences, Proceedings of the National Academy of Sciences, Vol. 98, No. 26 ( 2001-12-18), p. 15073-15077
Abstract:
After screening a bacterial artificial chromosome of human genomic
DNA library with human HS-40, ζ-, α-, and θ-globin probes, a 110-kb clone bearing the whole human α-globin gene cluster was
obtained and rare restriction endonuclease mapping was performed. The bacterial artificial chromosome DNA was isolated, and transgenic
mice were generated. Three founders were detected from 35 newborn mice. The copy numbers were 1, 2, and 2, and the expression of human
α-globin genes in various tissues at different developmental stages in the transgenic mice was assayed. The human α-globin mRNA can be
detected in bone marrow, kidney, liver, brain, but not in muscle, testis, or thymus. The human ζ-globin genes were switched off, and
the α-globin genes were switched at day 11.5 in mouse embryo, indicating that developmental stage-specific expression of the α-like
globin genes was properly regulated. The human α-globin mRNA ranged between 17–68% of the endogenous mouse α-globin,
suggesting that the expression of human α-globin genes is integration site-dependent in transgenic mice. The ratio of human α 2 -
and α 1 -globin gene expression in adult transgenic mouse
is about 2.5:1 similar to the expression in human.
Type of Medium:
Online Resource
ISSN:
0027-8424
,
1091-6490
DOI:
10.1073/pnas.251550598
Language:
English
Publisher:
Proceedings of the National Academy of Sciences
Publication Date:
2001
detail.hit.zdb_id:
209104-5
detail.hit.zdb_id:
1461794-8
SSG:
11
SSG:
12
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