In:
Journal of Virology, American Society for Microbiology, Vol. 74, No. 9 ( 2000-05), p. 4429-4432
Abstract:
Analysis of C57BL/6 mice acutely infected with lymphocytic choriomeningitis virus (LCMV) by using intracellular cytokine staining revealed a high frequency (2 to 10%) of CD4 + T cells secreting the Th1-associated cytokines interleukin-2 (IL-2), gamma interferon (IFN-γ), and tumor necrosis factor alpha, with no concomitant increase in the frequency of CD4 + T cells secreting the Th2-associated cytokines IL-4, IL-5, and IL-10 following stimulation with viral peptides. In LCMV-infected C57BL/6 CD8 −/− mice, more than 20% of the CD4 + T cells secreted IFN-γ after viral peptide stimulation, whereas less than 1% of the CD4 + T cells secreted IL-4 under these same conditions. Mice persistently infected with a high dose of LCMV clone 13 also generated a virtually exclusive Th1 response. Thus, LCMV induces a much more profound virus-specific CD4 + T-cell response than previously recognized, and it is dramatically skewed to a Th1 phenotype.
Type of Medium:
Online Resource
ISSN:
0022-538X
,
1098-5514
DOI:
10.1128/JVI.74.9.4429-4432.2000
Language:
English
Publisher:
American Society for Microbiology
Publication Date:
2000
detail.hit.zdb_id:
1495529-5
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