In:
European Journal of Immunology, Wiley, Vol. 24, No. 3 ( 1994-03), p. 503-507
Abstract:
After immunization of BALB/c mice with a low molecular mass fraction (FrD; ≦ 31 kDa) isolated from a soluble extract of Leishmania major promastigotes, a panel of monoclonal antibodies (mAb) was obtained. One of these antibodies (mAb 9C) recognized a cytosol‐associated antigen from L. major of approximately 21 kDa as shown by Western blot and immunoprecipitation. In addition, mAb 9C reacted with surface structures of murine splenic T cells and T cell clones. Reactivity was confined to murine cells, but was not strain restricted. Immunoprecipitation studies and surface‐labeling experiments with CD4 + T cell clones and the T cell receptor (TCR) − CD3 − T cell line TG40 transfected with V α/β chains from human TCR and concomitant co‐expression of murine CD3 suggested that mAb 9C binds to an epitope located within the murine CD3‐TCR complex. In addition, mAb 9C induced strong T cell proliferation. We conclude that L. major parasites share an epitope with the murine CD3‐TCR complex which is functionally important for T cell activation.
Type of Medium:
Online Resource
ISSN:
0014-2980
,
1521-4141
DOI:
10.1002/eji.1830240302
Language:
English
Publisher:
Wiley
Publication Date:
1994
detail.hit.zdb_id:
1491907-2
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