In:
Zeitschrift für Naturforschung C, Walter de Gruyter GmbH, Vol. 33, No. 1-2 ( 1978-2-1), p. 124-138
Abstract:
Further investigations on the structure of Friend murine leukemia virus (FLV) revealed that the transition from the “immature” (now termed “native”) to the structurally less organized “mature” (now termed “collapsed”) form occurs mainly as a result of the preparation for the electron microscope. A short pretreatment of virus with the detergent NP40 prior to negative staining with uranyl acetate is able to preserve the “native” structure of a high percentage of virus particles from standard preparations. Treatment with conventional fixatives was found to be in effective. Using this preparation technique, a more detailed electron microscopic analysis of the viral internal organization became possible. A thin layer designated “inner coat” was newly detected in close apposition to the viral membrane between the viral envelope and core. Removal of the unit membrane by more intensive detergent treatment suggests the existence of material extending be tween the viral surface knobs and the viral interior. The icosahedral core shell has an opening at one side and this opening matches with the hole in the apparently beehive-like arranged ribo-nucleoprotein (RNP) strand which represents the innermost structure. A comparative study of a series of representative mammalian C-type viruses with the same technique indicated a close similarity to Friend virus in fine structure, although differences in stability were observed. Based on these and earlier findings a model of the structure of mammalian C-type viruses is presented.
Type of Medium:
Online Resource
ISSN:
1865-7125
,
0939-5075
DOI:
10.1515/znc-1978-1-224
Language:
English
Publisher:
Walter de Gruyter GmbH
Publication Date:
1978
detail.hit.zdb_id:
2078107-6
SSG:
12
Bookmarklink