In:
American Journal of Veterinary Research, American Veterinary Medical Association (AVMA), Vol. 63, No. 7 ( 2002-07-01), p. 942-947
Abstract:
Objective —To determine whether a chemokine (RANTES)-like protein expressed by ciliary epithelium plays a role in uveitis. Sample Population —3 clinically normal horses intradermal, 5 eyes from 5 horses with recurrent uveitis, and 10 normal eyes from 5 age- and sex-matched horses. Procedure —Cross-reactivity and sensitivity of recombinant human (rh)-regulated upon activation, normal T-cell expressed and secreted (RANTES) protein were evaluated in horses by use of intradermal hypersensitivity reactions and a chemotaxis assay. Aqueous humor and ciliary body of eyes from clinically normal horses and horses with uveitis were examined for RANTES expression by use of an ELISA and reverse transcription-polymerase chain reaction (RT-PCR). Expression of RANTES mRNA and protein content of primary cultures of equine ciliary pigmented epithelial cells (RT-PCR) and culture supernatant (ELISA) were measured 6 or 24 hours, respectively, after cultures were stimulated with interleukin-1β and tumor necrosis factor-α. Results —Strong reactions to intradermal hypersensitivity testing and significant chemotaxis of equine leukocytes to rh-RANTES wereas observed. Aqueous humor of eyes from horses with uveitis contained increased concentrations of rh-RANTES-like protein (mean ± SD, 45.9 ± 31.7 pg/ml), compared with aqueous humor from clinically normal horses (0 pg/ml). Ciliary body from horses with uveitis expressed RANTES mRNA, whereas ciliary body from clinically normal horses had low mRNA expression. Stimulated ciliary pigmented epithelial cells expressed increased amounts of rh-RANTES-like protein (506.1 ± 298.3 pg/ml) and mRNA, compared with unstimulated samples. Conclusions and Clinical Relevance —Ciliary epithelium may play a role in recruitment and activation of leukocytes through expression of RANTES. ( Am J Vet Res 2002;63:942–947)
Type of Medium:
Online Resource
ISSN:
0002-9645
DOI:
10.2460/ajvr.2002.63.942
Language:
Unknown
Publisher:
American Veterinary Medical Association (AVMA)
Publication Date:
2002
detail.hit.zdb_id:
2056942-7
SSG:
22
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