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  • Hindawi Limited  (2)
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  • Hindawi Limited  (2)
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  • 1
    In: Mediators of Inflammation, Hindawi Limited, Vol. 2022 ( 2022-5-26), p. 1-20
    Abstract: Preeclampsia (PE) is a common pregnancy-related syndrome characterized by chronic immune activation. This study is aimed at exploring the role of miR-155 in the inflammatory pathogenesis of PE. Placental tissues and peripheral blood were collected from all subjects. BSP detection analysis was performed to evaluate miR-155 methylation levels. ELISA was performed to measure the levels of inflammatory cytokines and MMP2 in serum samples and cellular supernatants. HTR-8/SVneo and JEG-3 cells were transfected with miR-155 mimic and the inhibitor to establish the overexpressed miR-155 and silenced miR-155 cell models, respectively. Treatment with 5-Aza was performed to alter the DNA methylation level of miR-155. The PE rat model was established after subcutaneous injection of NG-nitro-L-arginine methyl ester. The CCK-8 assay, TUNEL staining, and Transwell assay were performed. Reverse transcription-quantitative PCR, Western blot analysis, and immunohistochemical assay were used to analyze related gene expression levels. The luciferase reporter assay was used to investigate the direct interaction between FOXO3 and miR-155. Results showed that miR-155 was remarkably upregulated and inversely correlated with the promoter methylation level in the placental tissue from PE patients. The in vitro experiments indicated that miR-155 decreased viability, migration, and invasion, but increased apoptosis in trophoblast cells. FOXO3 was confirmed as the target of miR-155. Transfection of the miR-155 inhibitor suppressed inflammation and oxidative stress, but elevated proliferation, migration, and invasion of trophoblast cells, which were abolished by 5-Aza treatment or cotransfection with si-FOXO3. In summary, our data suggested that methylation-mediated silencing of miR-155 can inhibit the apoptosis, inflammation, and oxidative stress of trophoblast cells by upregulating FOXO3.
    Type of Medium: Online Resource
    ISSN: 1466-1861 , 0962-9351
    Language: English
    Publisher: Hindawi Limited
    Publication Date: 2022
    detail.hit.zdb_id: 2008065-7
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  • 2
    Online Resource
    Online Resource
    Hindawi Limited ; 2021
    In:  Wireless Communications and Mobile Computing Vol. 2021 ( 2021-5-15), p. 1-13
    In: Wireless Communications and Mobile Computing, Hindawi Limited, Vol. 2021 ( 2021-5-15), p. 1-13
    Abstract: Severe weather and long-term driving of vehicles lead to various cracks on asphalt pavement. If these cracks cannot be found and repaired in time, it will have a negative impact on the safe driving of vehicles. Traditional artificial detection has some problems, such as low efficiency and missing detection. The detection model based on machine learning needs artificial design of pavement crack characteristics. According to the pavement distress identification manual proposed by the Federal Highway Administration (FHWA), these categories have three different types of cracks, such as fatigue, longitudinal crack, and transverse cracks. In the face of many types of pavement cracks, it is difficult to design a general feature extraction model to extract pavement crack features, which leads to the poor effect of the automatic detection model based on machine learning. Object detection based on the deep learning model has achieved good results in many fields. As a result, those models have become possible for pavement crack detection. This paper discusses the latest YOLOv5 series detection model for pavement crack detection and is to find out an effective training and detection method. Firstly, the 3001 asphalt crack pavement images with the original size of 2976 × 3978 pixels are collected using a digital camera and are randomly divided into three types according to the severity levels of low, medium, and high. Then, for the dataset of crack pavement, YOLOv5 series models are used for training and testing. The experimental results show that the detection accuracy of the YOLOv5l model is the highest, reaching 88.1%, and the detection time of the YOLOv5s model is the shortest, only 11.1 ms for each image.
    Type of Medium: Online Resource
    ISSN: 1530-8677 , 1530-8669
    Language: English
    Publisher: Hindawi Limited
    Publication Date: 2021
    detail.hit.zdb_id: 2045240-8
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