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  • International Union of Crystallography (IUCr)  (1)
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  • International Union of Crystallography (IUCr)  (1)
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    Online Resource
    Online Resource
    International Union of Crystallography (IUCr) ; 2015
    In:  Acta Crystallographica Section D Biological Crystallography Vol. 71, No. 10 ( 2015-10-01), p. 2054-2065
    In: Acta Crystallographica Section D Biological Crystallography, International Union of Crystallography (IUCr), Vol. 71, No. 10 ( 2015-10-01), p. 2054-2065
    Abstract: The selection of correct metal ions with high fidelity against competing cellular cations is crucial for the function of many metalloenzymes; however, the understanding of the principles that govern metal selectivity is still incomplete. In this study, the crystal structure of the Tm1162 protein from Thermotoga maritima , a metallo-β-lactamase, is reported. Several crystal structures of wild-type Tm1162 and its mutants were solved. Homologues of Tm1162 are widely distributed in bacteria and archaea, including several human pathogens. The monomer possesses an αβ/βα fold, with the core β-strands having the β-sheet sandwich structure common to the metallo-β-lactamase superfamily. Tm1162 exists as a trimer in the crystal and this trimeric unit is likely to be present in solution. In the trimer, three active sites reside at the interface between subunits, suggesting that the oligomeric assembly is crucial for catalysis. A new type of structurally encoded heterodinuclear site has been identified by confirming the identity of nickel-containing heteronuclear sites in Tm1162 via X-ray absorption spectroscopy and anomalous difference Fourier maps. The second coordination sphere, including His8 and Glu73, maintains the side-chain orientations of histidines and stabilizes the metal-binding site. Nickel coordination was crucial for the oligomerization of Tm1162. The nickel-dependent and manganese-dependent β-lactamase and phosphodiesterase activities of Tm1162 have also been characterized.
    Type of Medium: Online Resource
    ISSN: 1399-0047
    Language: Unknown
    Publisher: International Union of Crystallography (IUCr)
    Publication Date: 2015
    detail.hit.zdb_id: 2020492-9
    detail.hit.zdb_id: 2968623-4
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