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  • MDPI AG  (9)
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  • MDPI AG  (9)
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  • 1
    Online-Ressource
    Online-Ressource
    MDPI AG ; 2020
    In:  International Journal of Molecular Sciences Vol. 22, No. 1 ( 2020-12-30), p. 322-
    In: International Journal of Molecular Sciences, MDPI AG, Vol. 22, No. 1 ( 2020-12-30), p. 322-
    Kurzfassung: The leaf is an important photosynthetic organ and plays an essential role in the growth and development of plants. Leaf color mutants are ideal materials for studying chlorophyll metabolism, chloroplast development, and photosynthesis. In this study, we identified an EMS-induced mutant, yl2.1, which exhibited yellow cotyledons and true leaves that did not turn green with leaf growth. The yl2.1 locus was controlled by a recessive nuclear gene. The CsYL2.1 was mapped to a 166.7-kb genomic region on chromosome 2, which contains 24 predicted genes. Only one non-synonymous single nucleotide polymorphism (SNP) was found between yl2.1 and wt-WD1 that was located in Exon 7 of Csa2G263900, resulting in an amino acid substitution. CsYL2.1 encodes a plastid isoform of triose phosphate isomerase (pdTPI), which catalyzes the reversible conversion of dihydroxyacetone phosphate (DHAP) to glyceraldehyde-3-phosphate (GAP) in chloroplasts. CsYL2.1 was highly expressed in the cotyledons and leaves. The mesophyll cells of the yl2.1 leaves contained reduced chlorophyll and abnormal chloroplasts. Correspondingly, the photosynthetic efficiency of the yl2.1 leaves was impaired. Identification of CsYL2.1 is helpful in elucidating the function of ptTPI in the chlorophyll metabolism and chloroplast development and understanding the molecular mechanism of this leaf color variant in cucumber.
    Materialart: Online-Ressource
    ISSN: 1422-0067
    Sprache: Englisch
    Verlag: MDPI AG
    Publikationsdatum: 2020
    ZDB Id: 2019364-6
    SSG: 12
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 2
    In: Genes, MDPI AG, Vol. 11, No. 9 ( 2020-09-02), p. 1032-
    Kurzfassung: Lectin receptor-like kinases (LecRLKs) are a class of membrane proteins found in plants that are involved in diverse functions, including plant development and stress responses. Although LecRLK families have been identified in a variety of plants, a comprehensive analysis has not yet been undertaken in cucumber (Cucumis sativus L.). In this study, 46 putative LecRLK genes were identified in the cucumber genome, including 23 G-type and 22 L-type, and one C-type LecRLK gene. They were unequally distributed on all seven chromosomes, with a clustering tendency. Most of the genes in the cucumber LecRLK (CsLecRLK) gene family lacked introns. In addition, there were many regulatory elements associated with phytohormones and stress on these genes’ promoters. Transcriptome data demonstrated distinct expression patterns of CsLecRLK genes in various tissues. Furthermore, we found that each member of the CsLecRLK family had its own unique expression pattern under hormone and stress treatment by the quantitative real-time PCR (qRT-PCR) analysis. This study provides a better understanding of the character and function of the LecRLK gene family in cucumber and opens up the possibility to exploring the roles that LecRLKs might play in the life cycle of cucumber.
    Materialart: Online-Ressource
    ISSN: 2073-4425
    Sprache: Englisch
    Verlag: MDPI AG
    Publikationsdatum: 2020
    ZDB Id: 2527218-4
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 3
    In: Agronomy, MDPI AG, Vol. 12, No. 8 ( 2022-07-31), p. 1820-
    Kurzfassung: Salinity and alkalinity stress are two major constraints on plant growth and crop production, limiting sustainable agricultural production. Wheat is a vital cereal crop. It is very important to ensure food security; however, its growth and yield are usually adversely affected by salinity and alkalinity stress. To investigate the differential effects of neutral and alkaline salt stress on the seedling growth of wheat, we set wheat hydroponic culture experiment: CK, neutral salt (NaCl:Na2SO4 = 9:1 pH = 6.5), neutral salt with high pH value (NaCl:Na2SO4 = 9:1 pH = 8.9), alkaline salt (NaHCO3:Na2CO3 = 9:1 pH = 8.9), all treatments at the same Na+ concentration. The results indicated alkaline salt inhibited seedling growth more than neutral salt and neutral salt with high pH value. The results showed that the salt and alkali stresses decreased chlorophyll contents in leaves of wheat seedlings, inhibited photosynthesis and induced osmotic stress, oxidative stress and ion toxicity to wheat seedlings and finally inhibited the growth of wheat seedlings, while the alkaline salt caused a stronger injurious effect on wheat seedlings than the neutral salt, neutral salt with high pH value. Our study results demonstrated that alkaline salt inhibited wheat seedlings more significantly than neutral salt and neutral salt with high pH value. And, the main factor affected seedling growth is not pH alone.
    Materialart: Online-Ressource
    ISSN: 2073-4395
    Sprache: Englisch
    Verlag: MDPI AG
    Publikationsdatum: 2022
    ZDB Id: 2607043-1
    SSG: 23
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 4
    In: Cells, MDPI AG, Vol. 11, No. 23 ( 2022-12-01), p. 3874-
    Kurzfassung: A developmental niche vacancy in host embryos is necessary for stem cell complementation-based organ regeneration (SCOG). Thyroid transcription factor 1 (TTF-1) is a tissue-specific transcription factor that regulates the embryonic development and differentiation of the thyroid and, more importantly, lungs; thus, it has been considered as a master gene to knockout in order to develop a lung vacancy host. TTF-1 knockout mice were originally produced by inserting a stop codon in Exon 3 of the gene (E3stop) through embryonic stem cell-based homologous recombination. The main problems of utilizing E3stop host embryos for lung SCOG are that these animals all have a tracheoesophageal fistula (TEF), which cannot be corrected by donor stem cells, and most of them have monolateral sac-like lungs. To improve the mouse model towards achieving SCOG-based lung generation, in this project, we used the CRISPR/Cas9 tool to remove Exon 2 of the gene by zygote microinjection and successfully produced TTF-1 knockout (E2del) mice. Similar to E3stop, E2del mice are birth-lethal due to retarded lung development with sac-like lungs and only a rudimentary bronchial tree, increased basal cells but without alveolar type II cells and blood vessels, and abnormal thyroid development. Unlike E3stop, 57% of the E2del embryos presented type I tracheal agenesis (TA, a kind of human congenital malformation) with a shortened trachea and clear separations of the trachea and esophagus, while the remaining 43% had TEF. Furthermore, all the E2del mice had bilateral sac-like lungs. Both TA and bilateral sac-like lungs are preferred in SCOG. Our work presents a new strategy for producing SCOG host embryos that may be useful for lung regeneration.
    Materialart: Online-Ressource
    ISSN: 2073-4409
    Sprache: Englisch
    Verlag: MDPI AG
    Publikationsdatum: 2022
    ZDB Id: 2661518-6
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 5
    In: Sensors, MDPI AG, Vol. 23, No. 12 ( 2023-06-19), p. 5704-
    Kurzfassung: This paper presents the performance analysis of CentiSpace low earth orbit (LEO) experiment satellites. Distinguishing them from other LEO navigation augmentation systems, the co-time and co-frequency (CCST) self-interference suppression technique is employed in CentiSpace to mitigate significant self-interference caused by augmentation signals. Consequently, CentiSpace exhibits the capability of receiving navigation signals from the Global Navigation Satellite System (GNSS) while simultaneously broadcasting augmentation signals within the same frequency bands, thus ensuring excellent compatibility for GNSS receivers. CentiSpace is a pioneering LEO navigation system to successfully complete in-orbit verification of this technique. Leveraging the on-board experiment data, this study analyzes the performance of space-borne GNSS receivers equipped with self-interference suppression and evaluates the quality of navigation augmentation signals. The results show that CentiSpace space-borne GNSS receivers are capable of covering more than 90% visible GNSS satellites and the precision of self-orbit determination is at the centimeter level. Furthermore, the quality of augmentation signals meets the requirements outlined in the BDS interface control documents. These findings underscore the potential of the CentiSpace LEO augmentation system for the establishment of global integrity monitoring and GNSS signal augmentation. Moreover, these results contribute to subsequent research on LEO augmentation techniques.
    Materialart: Online-Ressource
    ISSN: 1424-8220
    Sprache: Englisch
    Verlag: MDPI AG
    Publikationsdatum: 2023
    ZDB Id: 2052857-7
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 6
    Online-Ressource
    Online-Ressource
    MDPI AG ; 2022
    In:  Applied Sciences Vol. 12, No. 22 ( 2022-11-10), p. 11396-
    In: Applied Sciences, MDPI AG, Vol. 12, No. 22 ( 2022-11-10), p. 11396-
    Kurzfassung: Aiming at the problem of the wide range and great difficulty in the future of battlefield airspace control, based on the unique advantages of an airspace grid system in an airspace grid representation and time–space binary computing, this paper designs a pre-clustering method for mission airspace based on airspace location correlation under the condition of future large-scale air combat missions in order to realize the block control of battlefield airspace. This method reduces the whole 3D battlefield space projection to a 2D plane and regards the task airspace projection as “obstacles” in the task area; Multi-A * algorithm is used to generate the airspace clustering line surrounding the task airspace, and the airspace association clustering problem is transformed into a multiple “start point-end point” path planning problem with autonomous optimization. Through the experiment, it was found that clustering the airspace can effectively improve the management and control efficiency of large-scale battlefield airspace.
    Materialart: Online-Ressource
    ISSN: 2076-3417
    Sprache: Englisch
    Verlag: MDPI AG
    Publikationsdatum: 2022
    ZDB Id: 2704225-X
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  • 7
    Online-Ressource
    Online-Ressource
    MDPI AG ; 2023
    In:  Diagnostics Vol. 13, No. 19 ( 2023-09-24), p. 3034-
    In: Diagnostics, MDPI AG, Vol. 13, No. 19 ( 2023-09-24), p. 3034-
    Kurzfassung: Crush syndrome (CS), also known as traumatic rhabdomyolysis, is a syndrome with a wide clinical spectrum; it is caused by external compression, which often occurs in earthquakes, wars, and traffic accidents, especially in large-scale disasters. Crush syndrome is the second leading cause of death after direct trauma in earthquakes. A series of clinical complications caused by crush syndrome, including hyperkalemia, myoglobinuria, and, in particular, acute kidney injury (AKI), is the main cause of death in crush syndrome. The early diagnosis of crush syndrome, the correct evaluation of its severity, and accurate predictions of a poor prognosis can provide personalized suggestions for rescuers to carry out early treatments and reduce mortality. This review summarizes various methods for the diagnostic and predictive evaluation of crush syndrome, including urine dipstick tests for a large number of victims, traditional and emerging biomarkers, imaging-assisted diagnostic methods, and developed evaluation models, with the aim of providing materials for scholars in this research field.
    Materialart: Online-Ressource
    ISSN: 2075-4418
    Sprache: Englisch
    Verlag: MDPI AG
    Publikationsdatum: 2023
    ZDB Id: 2662336-5
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 8
    Online-Ressource
    Online-Ressource
    MDPI AG ; 2019
    In:  Applied Sciences Vol. 9, No. 5 ( 2019-03-11), p. 1004-
    In: Applied Sciences, MDPI AG, Vol. 9, No. 5 ( 2019-03-11), p. 1004-
    Kurzfassung: In recent years, the formation control of multi-mobile robots has been widely investigated by researchers. With increasing numbers of robots in the formation, distributed formation control has become the development trend of multi-mobile robot formation control, and the consensus problem is the most basic problem in the distributed multi-mobile robot control algorithm. Therefore, it is very important to analyze the consensus of multi-mobile robot systems. There are already mature and sophisticated strategies solving the consensus problem in ideal environments. However, in practical applications, uncertain factors like communication noise, communication delay and measurement errors will still lead to many problems in multi-robot formation control. In this paper, the consensus problem of second-order multi-robot systems with multiple time delays and noises is analyzed. The characteristic equation of the system is transformed into a quadratic polynomial of pure imaginary eigenvalues using the frequency domain analysis method, and then the critical stability state of the maximum time delay under noisy conditions is obtained. When all robot delays are less than the maximum time delay, the system can be stabilized and achieve consensus. Compared with the traditional Lyapunov method, this algorithm has lower conservativeness, and it is easier to extend the results to higher-order multi-robot systems. Finally, the results are verified by numerical simulation using MATLAB/Simulink. At the same time, a multi-mobile robot platform is built, and the proposed algorithm is applied to an actual multi-robot system. The experimental results show that the proposed algorithm is finally able to achieve the consensus of the second-order multi-robot system under delay and noise interference.
    Materialart: Online-Ressource
    ISSN: 2076-3417
    Sprache: Englisch
    Verlag: MDPI AG
    Publikationsdatum: 2019
    ZDB Id: 2704225-X
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 9
    In: Genes, MDPI AG, Vol. 11, No. 2 ( 2020-02-06), p. 171-
    Kurzfassung: Cucumber is one of the most important vegetables in the world. The C2H2 zinc finger protein (C2H2-ZFP) family plays an important role in the growth development and abiotic stress responses of plants. However, there have been no systematic studies on cucumber. In this study, we performed a genome-wide study of C2H2-ZFP genes and analyzed their chromosomal location, gene structure, conservation motif, and transcriptional expression. In total, 101 putative cucumber C2H2-ZFP genes were identified and divided into six groups (I–VI). RNA-seq transcriptome data on different organs revealed temporal and spatial expression specificity of the C2H2-ZFP genes. Expression analysis of sixteen selected C2H2-ZFP genes in response to cold, drought, salt, and abscisic acid (ABA) treatments by real-time quantitative polymerase chain reaction showed that C2H2-ZFP genes may be involved in different signaling pathways. These results provide valuable information for studying the function of cucumber C2H2-ZFP genes in the future.
    Materialart: Online-Ressource
    ISSN: 2073-4425
    Sprache: Englisch
    Verlag: MDPI AG
    Publikationsdatum: 2020
    ZDB Id: 2527218-4
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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