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  • 1
    In: Animals, MDPI AG, Vol. 13, No. 12 ( 2023-06-19), p. 2032-
    Abstract: Brachycephalic breeds have increased in popularity despite growing awareness of their predisposition to a wide range of conformation-related diseases. The extreme facial conformation of many popular brachycephalic breeds compromises their ocular surface health, increasing the risk of painful corneal ulceration. Medial canthoplasty (MC) is a surgical procedure to address ocular abnormalities in brachycephalic dogs, which are collectively referred to as brachycephalic ocular syndrome (BOS). This study retrospectively reviewed the records of dogs recommended MC at a referral hospital between 2016 and 2021. A questionnaire was designed to identify owners’ perceptions pre- and post-operatively. From 271 brachycephalic dogs recommended MC, 43.5% (118/271) underwent surgery and 72.0% (85/118) were Pugs. The majority of dogs (73.7%, 87/118) that underwent surgery had current or historical corneal ulceration. Follow-up was available in 104 dogs, of which 5.7% (6/104) had corneal ulceration post-operatively. Sixty-four owners completed the questionnaire and reported post-operative corneal ulceration in 12.5% of dogs (8/64), reduced ocular discharge (70.8%, 34/48), reduced ocular irritation (67.7%, 21/31) and less periocular cleaning (52.5%, 32/61). Owners were satisfied with the clinical (85.9%, 55/64) and cosmetic (87.5%, 56/64) outcome. In conclusion, MC has high clinical relevance for the surgical management of BOS, restoring functional conformation and improving the quality of life of affected dogs.
    Type of Medium: Online Resource
    ISSN: 2076-2615
    Language: English
    Publisher: MDPI AG
    Publication Date: 2023
    detail.hit.zdb_id: 2606558-7
    SSG: 23
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  • 2
    In: Animals, MDPI AG, Vol. 8, No. 2 ( 2018-02-20), p. 30-
    Type of Medium: Online Resource
    ISSN: 2076-2615
    Language: English
    Publisher: MDPI AG
    Publication Date: 2018
    detail.hit.zdb_id: 2606558-7
    SSG: 23
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  • 3
    In: Antibiotics, MDPI AG, Vol. 9, No. 11 ( 2020-11-04), p. 775-
    Abstract: Group A Streptococcus (GAS) causes 700 million infections and accounts for half a million deaths per year. Antibiotic treatment failure rates of 20–40% have been observed. The role host cell glycans play in GAS biofilm formation in the context of GAS pharyngitis and subsequent antibiotic treatment failure has not been previously investigated. GAS serotype M12 GAS biofilms were assessed for biofilm formation on Detroit 562 pharyngeal cell monolayers following enzymatic removal of all N-linked glycans from pharyngeal cells with PNGase F. Removal of N-linked glycans resulted in an increase in biofilm biomass compared to untreated controls. Further investigation into the removal of terminal mannose and sialic acid residues with α1-6 mannosidase and the broad specificity sialidase (Sialidase A) also found that biofilm biomass increased significantly when compared to untreated controls. Increases in biofilm biomass were associated with increased production of extracellular polymeric substances (EPS). Furthermore, it was found that M12 GAS biofilms grown on untreated pharyngeal monolayers exhibited a 2500-fold increase in penicillin tolerance compared to planktonic GAS. Pre-treatment of monolayers with exoglycosidases resulted in a further doubling of penicillin tolerance in resultant biofilms. Lastly, an additional eight GAS emm-types were assessed for biofilm formation in response to terminal mannose and sialic acid residue removal. As seen for M12, biofilm biomass on monolayers increased following removal of terminal mannose and sialic acid residues. Collectively, these data demonstrate that pharyngeal cell surface glycan structures directly impact GAS biofilm formation in a strain and glycan specific fashion.
    Type of Medium: Online Resource
    ISSN: 2079-6382
    Language: English
    Publisher: MDPI AG
    Publication Date: 2020
    detail.hit.zdb_id: 2681345-2
    SSG: 15,3
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  • 4
    In: Plants, MDPI AG, Vol. 11, No. 20 ( 2022-10-12), p. 2685-
    Abstract: Osmorhiza occidentalis Torr. is an essential-oil-bearing plant in the Apiaceae family. Volatile oil was produced through steam distillation (n = 3) of the above ground plant parts and was analyzed by gas chromatography (GC/FID, GC/MS), and gas chromatography/isotope ratio mass spectrometry (GC/IRMS) to establish the essential oil composition and stable isotope profile. The resulting essential oils were found to be comprised of 33 volatile compounds. Prominent volatile compounds include methyl chavicol (avg. 61.6%), (Z)-β-ocimene (avg. 14.7%), sabinene (avg. 10.5%), and γ-terpinene (avg. 2.8%). Stable isotope values were determined for prominent volatile compounds, including methyl chavicol, (Z)-β-ocimene, sabinene, and γ-terpinene. Values for δ2H range from −393.479 (avg. sabinene) to −171.516 (avg. methyl chavicol). Those for δ13C range from −35.957 (avg. methyl chavicol) to −30.820 (avg. (Z)-β-ocimene). The essential oil yield was 0.12% (w/w). The current study establishes for the first time, to the best knowledge of the authors, the essential oil yield, essential oil composition, and stable isotope profile of prominent volatile compounds extracted from the above-ground portions of O. occidentalis. These results provide insight into the volatile chemical composition produced by the plant and provide fundamental data for substantiation of ethnobotanical applications.
    Type of Medium: Online Resource
    ISSN: 2223-7747
    Language: English
    Publisher: MDPI AG
    Publication Date: 2022
    detail.hit.zdb_id: 2704341-1
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  • 5
    In: Nutrients, MDPI AG, Vol. 14, No. 3 ( 2022-01-19), p. 434-
    Abstract: Celebrities, including influencers, are commonly used to market products that are high in fat, sugar, and salt (HFSS) to children but the impact on dietary outcomes has been unclear. The primary aim of this study was to systematically review the literature and quantify the impact of celebrities in HFSS marketing on children’s dietary outcomes. We searched eight databases and included studies from all countries and languages published from 2009 until August 2021. Participants were defined as under 16 years, exposure was marketing for HFSS products with a celebrity, and the outcomes were dietary preference, purchasing behaviors, and consumption of HFSS products. We were able to conduct a meta-analysis for consumption outcomes. Seven articles met the inclusion criteria, of which three were included in the meta-analysis. Under experimental conditions, the use of celebrities in HFSS marketing compared to non-food marketing was found to significantly increase consumption of the marketed HFSS product by 56.4 kcals (p = 0.021). There was limited evidence on the impact on preference or purchase intentions and on the comparisons between use and non-use of celebrities and influencers.
    Type of Medium: Online Resource
    ISSN: 2072-6643
    Language: English
    Publisher: MDPI AG
    Publication Date: 2022
    detail.hit.zdb_id: 2518386-2
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  • 6
    In: Animals, MDPI AG, Vol. 12, No. 5 ( 2022-03-02), p. 629-
    Abstract: The UK recorded sharp rises in puppy purchasing during the 2020 phase of the COVID-19 pandemic, with many first-time dog owners purchasing puppies to improve their mental health during this challenging period. Government restrictions on movement and social interaction during the pandemic led to animal welfare concerns over puppies’ reduced time-sensitive exposures to key environmental and social stimuli during their critical developmental period. This study aimed to compare demographics, health and early-life experiences of puppies purchased and brought home 〈 16 weeks of age between 23 March–31 December 2020 (“Pandemic Puppies”), with dogs purchased and brought home 〈 16 weeks during the same date period in 2019 (“2019 puppies”). An online survey of UK-based puppy owners was conducted between 10 November and 31 December 2020 with valid responses representing 5517 puppies (Pandemic Puppies: n = 4369; 2019 puppies: n = 1148). Multivariable logistic regression modelling revealed that Pandemic Puppies were less likely to have attended puppy training classes (67.9% 2019 vs. 28.9% 2020; p 〈 0.001) or had visitors to their home (94.5% 2019 vs. 81.8% 2020; p 〈 0.001) aged 〈 16 weeks compared with 2019 puppies. Fewer Pandemic Puppies underwent veterinary checks prior to purchase than 2019 puppies (2019: 91.3% vs. 2020: 87.4%; p 〈 0.001), but more were sold with a passport (2019: 4.1% vs. 2020: 7.1%; p 〈 0.001). Pandemic Puppies were significantly more likely to be ‘Designer Crossbreeds’ (2019: 18.8% vs. 2020: 26.1%; p 〈 0.001) and less likely to be Kennel Club registered than 2019 puppies (2019: 58.2% vs. 2020: 46.2%; p 〈 0.001). Greater support from veterinary and animal behavioural professionals is likely needed to ameliorate the health and behavioural impacts of growing up in a pandemic upon this vulnerable population.
    Type of Medium: Online Resource
    ISSN: 2076-2615
    Language: English
    Publisher: MDPI AG
    Publication Date: 2022
    detail.hit.zdb_id: 2606558-7
    SSG: 23
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  • 7
    In: Nutrients, MDPI AG, Vol. 13, No. 3 ( 2021-03-10), p. 900-
    Abstract: Front of pack food labels (FOPLs) provide accessible nutritional information to guide consumer choice. Using an online experiment with a large representative British sample, we aimed to examine whether FOPLs improve participants’ ability to identify the healthiness of foods and drinks. The primary aim was to compare ability to rank between FOPL groups and a no label control. Adults (≥18 years), recruited from the NatCen panel, were randomised to one of five experimental groups (Multiple Traffic Light, MTL; Nutri-Score, N-S; Warning Label, WL; Positive Choice tick, PC; no label control). Stratification variables were year of recruitment to panel, sex, age, government office region, and household income. Packaging images were created for three versions, varying in healthiness, of six food and drink products (pizza, drinks, cakes, crisps, yoghurts, breakfast cereals). Participants were asked to rank the three product images in order of healthiness. Ranking was completed on a single occasion and comprised a baseline measure (with no FOPL), and a follow-up measure including the FOPL as per each participant’s experimental group. The primary outcome was the ability to accurately rank product healthiness (all products ranked correctly vs. any incorrect). In 2020, 4504 participants had complete data and were included in the analysis. The probability of correct ranking at follow-up, and improving between baseline and follow-up, was significantly greater across all products for the N-S, MTL and WL groups, compared to control. This was seen for only some of the products for the PC group. The largest effects were seen for N-S, followed by MTL. These analyses were adjusted for stratification variables, ethnicity, education, household composition, food shopping responsibility, and current FOPL use. Exploratory analyses showed a tendency for participants with higher compared to lower education to rank products more accurately. Conclusions: All FOPLs were effective at improving participants’ ability to correctly rank products according to healthiness in this large representative British sample, with the largest effects seen for N-S, followed by MTL.
    Type of Medium: Online Resource
    ISSN: 2072-6643
    Language: English
    Publisher: MDPI AG
    Publication Date: 2021
    detail.hit.zdb_id: 2518386-2
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  • 8
    In: Molecules, MDPI AG, Vol. 27, No. 9 ( 2022-04-27), p. 2777-
    Abstract: Ocimum campechianum Mill. (Peruvian basil) is an essential oil-bearing plant of the Lamiaceae family. Volatile oil produced through steam distillation of Peruvian basil was examined to establish the aromatic and stable isotope profiles of samples (n = 9) from three different cultivated plots in Peru. The resulting essential oils were analyzed by GC/FID, GC/MS, and GC/IRMS. In accordance with findings from other researchers, multiple chemotypes, defined by the most abundant aromatic compounds, exist within these populations. Overall, 55% of samples are the eugenol chemotype (values ranging 15.4–30.2%), 33% are the methyl eugenol chemotype (values ranging 68.1–68.7%), and a single sample is a mixture of both chemotypes, containing high levels of both eugenol (38.1%) and methyl eugenol (8.6%). Stable isotope ratios, δ2H and δ13C, performed on prominent compounds provide supporting data for distinguishing chemotypes. Complete aromatic profiles, stable isotope ratios, and essential oil yield are established for each sample. This study confirms the existence of multiple chemotypes and, for the first time, to the author’s best knowledge, establishes stable isotope ratios for O. campechianum essential oil, which proves a useful tool in further investigating plant metabolism and determining essential oil authenticity.
    Type of Medium: Online Resource
    ISSN: 1420-3049
    Language: English
    Publisher: MDPI AG
    Publication Date: 2022
    detail.hit.zdb_id: 2008644-1
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  • 9
    In: Molecules, MDPI AG, Vol. 26, No. 8 ( 2021-04-17), p. 2343-
    Abstract: Lavandula angustifolia Mill. (lavender) is an essential-oil-bearing plant in the Lamiaceae family. Volatile oil produced through the steam distillation of lavender was examined to establish the essential oil yield and aromatic profile from each portion of the plant—namely, the corolla, calyx, leaf, and whole flowering top. The resulting essential oils were analyzed by GC-FID and GC-MS. The different plant parts generally shared similar compounds but in varying relative percentages. Aromatic profiles of the whole flowering top and calyx were similar, with prominent compounds being linalool acetate (34.3%, 32.0%), linalool (26.5%, 32.9%), lavandulyl acetate (5.6%, 4.9%), terpinen-4-ol (5.3%, 7.0%), and (Z)-β-ocimene (4.5%, 5.4%), respectively. Aromatic profiles for the corolla and leaf were unique. Prominent aromatic compounds of the corolla included linalool acetate (18.4%), linalool (10.8%), epi-α-cadinol (10.0%), borneol (7.3%), and lavandulyl acetate (6.3%). Prominent aromatic compounds of the leaf included epi-α-cadinol (19.8%), γ-cadinene (11.0%), borneol (6.0%), caryophyllene oxide (4.9%), and bornyl acetate (4.8%). Complete profiles and essential oil yields of corolla, calyx, leaf, and whole flowering top were established. This study establishes the influence the corolla, calyx, and leaf exert on the aromatic profile of the whole flowering top and provides insight into authentication of lavender essential oil.
    Type of Medium: Online Resource
    ISSN: 1420-3049
    Language: English
    Publisher: MDPI AG
    Publication Date: 2021
    detail.hit.zdb_id: 2008644-1
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  • 10
    In: Animals, MDPI AG, Vol. 11, No. 9 ( 2021-08-25), p. 2500-
    Abstract: Widespread media reports suggest that unusually high numbers of the public purchased, or sought to purchase, puppies following the first ‘lockdown’ phase of the COVID-19 pandemic in the UK, dubbed “Pandemic Puppies”. This study aimed to explore this phenomenon by comparing pre-purchase motivations and behaviours, and purchase behaviours of UK owners purchasing puppies aged 〈 16 weeks from 23 March 2020–31 December 2020 with those of owners who purchased puppies during the same date-period in 2019. An online survey was conducted during November-December 2020, from which 5517 valid responses were analysed (2019 puppies: n = 1148; 2020 ‘Pandemic Puppies’: n = 4369). Over 1 in 10 Pandemic Puppy owners had not considered purchasing a puppy before the pandemic, and 2 in 5 felt their decision to purchase a puppy had been influenced by the pandemic, most commonly by having more time to care for a dog (86.7%). Multivariable logistic regression models revealed that Pandemic Puppy owners were more likely to be first-time dog owners and have children in their household, were less likely to seek out a breeder that performed health testing on their breeding dog(s) or view their puppy in-person, and were more likely to pay a deposit without seeing their puppy. At purchase, Pandemic Puppies were more likely to be younger, delivered or collected from outside their breeders’ property, seen without their littermates, and cost 〉 £2000 compared with 2019 puppies. Changes in puppy purchasing during the pandemic raise welfare concerns for this unique population, including relinquishment, behavioural problems and poor health.
    Type of Medium: Online Resource
    ISSN: 2076-2615
    Language: English
    Publisher: MDPI AG
    Publication Date: 2021
    detail.hit.zdb_id: 2606558-7
    SSG: 23
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