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  • MDPI AG  (152)
  • 1
    In: Nutrients, MDPI AG, Vol. 15, No. 2 ( 2023-01-11), p. 360-
    Abstract: Background: Targeting the gut microbiota may become a new therapeutic to prevent and delay the progression of chronic kidney disease (CKD). Nonetheless, the causal relationship between specific intestinal flora and CKD is still unclear. Materials and Method: To identify genetically predicted microbiota, we used summary data from genome-wide association studies on gut microbiota in 18340 participants from 24 cohorts. Furthermore, we genetically predicted the causal relationship between 211 gut microbiotas and six phenotypes (outcomes) (CKD, estimated glomerular filtration rate (eGFR), urine albumin to creatinine ratio (UACR), dialysis, rapid progress to CKD, and rapid decline of eGFR). Four Mendelian randomization (MR) methods, including inverse variance weighted (IVW), MR-Egger, weighted median, and weighted mode were used to investigate the casual relationship between gut microbiotas and various outcomes. The result of IVW was deemed as the primary result. Then, Cochrane’s Q test, MR-Egger, and MR-PRESSO Global test were used to detect heterogeneity and pleiotropy. The leave-one method was used for testing the stability of MR results and Bonferroni-corrected was used to test the strength of the causal relationship between exposure and outcome. Results: Through the MR analysis of 211 microbiotas and six clinical phenotypes, a total of 36 intestinal microflora were found to be associated with various outcomes. Among them, Class Bacteroidia (=−0.005, 95% CI: −0.001 to −0.008, p = 0.002) has a strong causality with lower eGFR after the Bonferroni-corrected test, whereas phylum Actinobacteria (OR = 1.0009, 95%CI: 1.0003–1.0015, p = 0.0024) has a strong causal relationship with dialysis. The Cochrane’s Q test reveals that there is no significant heterogeneity between various single nucleotide polymorphisms. In addition, no significant level of pleiotropy was found according to MR-Egger and MR-PRESSO Global tests. Conclusions: Through the two-sample MR analysis, we identified the specific intestinal flora that has a causal relationship with the incidence and progression of CKD at the level of gene prediction, which may provide helpful biomarkers for early disease diagnosis and potential therapeutic targets for CKD.
    Type of Medium: Online Resource
    ISSN: 2072-6643
    Language: English
    Publisher: MDPI AG
    Publication Date: 2023
    detail.hit.zdb_id: 2518386-2
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  • 2
    In: Journal of Clinical Medicine, MDPI AG, Vol. 12, No. 6 ( 2023-03-13), p. 2226-
    Abstract: Background: Extensive knowledge of allergic multimorbidities is required to improve the management of allergic diseases with the industrialization of China. However, the demography and allergen distribution patterns of allergic multimorbidities in China remain unclear, despite the increasing prevalence of allergies. Methods: This was a real-world, cross-sectional study of 1273 outpatients diagnosed with one or more allergic diseases in Guangzhou, the most populated city of southern China, with leading industrial and commercial centers, between April 2021 and March 2022. Seven allergic diseases (allergic rhinitis (AR), asthma (AS)/cough variant asthma (CVA), atopic dermatitis (AD)/eczema, food allergy (FA), allergic conjunctivitis (AC), drug allergy (DA), and anaphylaxis) were assessed. Positive rates of sensitization to different allergens were measured using an allergen detection system of the UniCAP (Pharmacia Diagnostics, Sweden) instrument platform to compare the groups of allergic multimorbidities against a single entity. Results: There were 659 (51.8%) males and 614 (48.2%) females aged from 4 months to 74 years included in the analysis. The study participants who were diagnosed with allergic diseases had an average of 1.6 diagnoses. Overall, 46.5% (592 of 1273) of the patients had more than one allergic condition, and allergic rhinitis was the most common type of multimorbidity. Women were more likely to suffer from an allergic disease alone, whereas allergic multimorbidities were more likely to be diagnosed in men (p = 0.005). In addition, allergic multimorbidities were common in all age groups, with an incidence ranging from 37.1% to 57.4%, in which children and adolescents were more frequently diagnosed with allergic multimorbidities than adults (18–60 years old) (all p 〈 0.05). Allergic multimorbidity was observed throughout the year. A difference in the positive rate of allergens sensitization and total immunoglobulin E (tIgE) levels between different allergic multimorbidities was observed. Conclusions: Allergic multimorbidities were very commonly found in nearly half of all patients with allergies. The proportion of allergic multimorbidities varied with the type of disease, sex, age, and allergen distribution pattern. These findings may help clinicians to develop “One health” strategies for the clinical management of allergic diseases.
    Type of Medium: Online Resource
    ISSN: 2077-0383
    Language: English
    Publisher: MDPI AG
    Publication Date: 2023
    detail.hit.zdb_id: 2662592-1
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  • 3
    Online Resource
    Online Resource
    MDPI AG ; 2022
    In:  Remote Sensing Vol. 14, No. 10 ( 2022-05-20), p. 2460-
    In: Remote Sensing, MDPI AG, Vol. 14, No. 10 ( 2022-05-20), p. 2460-
    Abstract: Forest fires are among the biggest threats to forest ecosystems and forest resources, and can lead to ecological disasters and social crises. Therefore, it is imperative to detect and extinguish forest fires in time to reduce their negative impacts. Satellite remote sensing, especially meteorological satellites, has been a useful tool for forest-fire detection and monitoring because of its high temporal resolution over large areas. Researchers monitor forest fires directly at pixel level, which usually presents a mixture of forest and fire, but the low spatial resolution of such mixed pixels cannot accurately locate the exact position of the fire, and the optimal time window for fire suppression can thus be missed. In order to improve the positioning accuracy of the origin of forest fire (OriFF), we proposed a mixed-pixel unmixing integrated with pixel-swapping algorithm (MPU-PSA) model to monitor the OriFFs in time. We then applied the model to the Japanese Himawari-8 Geostationary Meteorological Satellite data to obtain forest-fire products at subpixel level. In this study, the ground truth data were provided by the Department of Emergency Management of Hunan Province, China. To validate the positioning accuracy of MPU-PSA for OriFFs, we applied the model to the Himawari-8 satellite data and then compared the derived fire results with fifteen reference forest-fire events that occurred in Hunan Province, China. The results show that the extracted forest-fire locations using the proposed method, referred to as forest fire locations at subpixel (FFLS) level, were far closer to the actual OriFFs than those from the modified Himawari-8 Wild Fire Product (M-HWFP). This improvement will help to reduce false fire claims in the Himawari-8 Wild Fire Product (HWFP). We conducted a comparative study of M-HWFP and FFLS products using three accuracy-evaluation indexes, i.e., Euclidean distance, RMSE, and MAE. The mean distances between M-HWFP fire locations and OriFFs and between FFLS fire locations and OriFFs were 3362.21 m and 1294.00 m, respectively. The mean RMSEs of the M-HWFP and FFLS products are 1225.52 m and 474.93 m, respectively. The mean MAEs of the M-HWFP and FFLS products are 992.12 m and 387.13 m, respectively. We concluded that the newly proposed MPU-PSA method can extract forest-fire locations at subpixel level, providing higher positioning accuracy of forest fires for their suppression.
    Type of Medium: Online Resource
    ISSN: 2072-4292
    Language: English
    Publisher: MDPI AG
    Publication Date: 2022
    detail.hit.zdb_id: 2513863-7
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  • 4
    In: Remote Sensing, MDPI AG, Vol. 14, No. 15 ( 2022-07-24), p. 3546-
    Abstract: The accurate detection of burned forest area is essential for post-fire management and assessment, and for quantifying carbon budgets. Therefore, it is imperative to map burned areas accurately. Currently, there are few burned-area products around the world. Researchers have mapped burned areas directly at the pixel level that is usually a mixture of burned area and other land cover types. In order to improve the burned area mapping at subpixel level, we proposed a Burned Area Subpixel Mapping (BASM) workflow to map burned areas at the subpixel level. We then applied the workflow to Sentinel 2 data sets to obtain burned area mapping at subpixel level. In this study, the information of true fire scar was provided by the Department of Emergency Management of Hunan Province, China. To validate the accuracy of the BASM workflow for detecting burned areas at the subpixel level, we applied the workflow to the Sentinel 2 image data and then compared the detected burned area at subpixel level with in situ measurements at fifteen fire-scar reference sites located in Hunan Province, China. Results show the proposed method generated successfully burned area at the subpixel level. The methods, especially the BASM-Feature Extraction Rule Based (BASM-FERB) method, could minimize misclassification and effects due to noise more effectively compared with the BASM-Random Forest (BASM-RF), BASM-Backpropagation Neural Net (BASM-BPNN), BASM-Support Vector Machine (BASM-SVM), and BASM-notra methods. We conducted a comparison study among BASM-FERB, BASM-RF, BASM-BPNN, BASM-SVM, and BASM-notra using five accuracy evaluation indices, i.e., overall accuracy (OA), user’s accuracy (UA), producer’s accuracy (PA), intersection over union (IoU), and Kappa coefficient (Kappa). The detection accuracy of burned area at the subpixel level by BASM-FERB’s OA, UA, IoU, and Kappa is 98.11%, 81.72%, 74.32%, and 83.98%, respectively, better than BASM-RF’s, BASM-BPNN’s, BASM-SVM’s, and BASM-notra’s, even though BASM-RF’s and BASM-notra’s average PA is higher than BASM-FERB’s, with 89.97%, 91.36%, and 89.52%, respectively. We conclude that the newly proposed BASM workflow can map burned areas at the subpixel level, providing greater accuracy in regards to the burned area for post-forest fire management and assessment.
    Type of Medium: Online Resource
    ISSN: 2072-4292
    Language: English
    Publisher: MDPI AG
    Publication Date: 2022
    detail.hit.zdb_id: 2513863-7
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  • 5
    Online Resource
    Online Resource
    MDPI AG ; 2018
    In:  International Journal of Molecular Sciences Vol. 19, No. 10 ( 2018-10-04), p. 3023-
    In: International Journal of Molecular Sciences, MDPI AG, Vol. 19, No. 10 ( 2018-10-04), p. 3023-
    Abstract: Sipunculus nudus, an old marine species, has great potential for use as functional seafood due to its various bioactivities. Its potential antithrombotic activity pushed us to isolate the bio-active components bio-guided by tracking fibrinolytic activity. As a result, a novel protease named as SK (the kinase obtained from S. nudus) was obtained, which possessed a molecular weight of 28,003.67 Da and 15 N-terminal amino acid sequences of PFPVPDPFVWDTSFQ. SK exerted inhibitory effects on thrombus formation through improving the coagulation system with dose-effect relationship within a certain range. Furthermore, in most cases SK got obviously better effect than that of urokinase. With the help of untargeted mass spectrometry-based metabolomics profiling, arachidonic acid, sphingolipid, and nicotinate and nicotinamide mechanism pathways were found to be important pathways. They revealed that the effect mechanism of SK on common carotid arterial thrombosis induced by FeCl3 was achieved by inhibiting vessel contraction, platelet aggregation, adhesion, and release, correcting endothelial cell dysfunction and retarding process of thrombus formation. This study demonstrated SK was a promising thrombolytic agent on the basis of its comprehensive activities on thrombosis, and it should get further exploitation and utilization.
    Type of Medium: Online Resource
    ISSN: 1422-0067
    Language: English
    Publisher: MDPI AG
    Publication Date: 2018
    detail.hit.zdb_id: 2019364-6
    SSG: 12
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  • 6
    In: International Journal of Environmental Research and Public Health, MDPI AG, Vol. 14, No. 6 ( 2017-06-07), p. 616-
    Type of Medium: Online Resource
    ISSN: 1660-4601
    Language: English
    Publisher: MDPI AG
    Publication Date: 2017
    detail.hit.zdb_id: 2175195-X
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  • 7
    Online Resource
    Online Resource
    MDPI AG ; 2021
    In:  Energies Vol. 14, No. 19 ( 2021-10-07), p. 6404-
    In: Energies, MDPI AG, Vol. 14, No. 19 ( 2021-10-07), p. 6404-
    Abstract: Based on outlier detection algorithms, a feasible quantification method for supraharmonic emission signals is presented. It is designed to tackle the requirements of high-resolution and low data volume simultaneously in the frequency domain. The proposed method was developed from the skewed distribution data model and the self-tuning parameters of density-based spatial clustering of applications with noise (DBSCAN) algorithm. Specifically, the data distribution of the supraharmonic band was analyzed first by the Jarque–Bera test. The threshold was determined based on the distribution model to filter out noise. Subsequently, the DBSCAN clustering algorithm parameters were adjusted automatically, according to the k-dist curve slope variation and the dichotomy parameter seeking algorithm, followed by the clustering. The supraharmonic emission points were analyzed as outliers. Finally, simulated and experimental data were applied to verify the effectiveness of the proposed method. On the basis of the detection results, a spectrum with the same resolution as the original spectrum was obtained. The amount of data declined by more than three orders of magnitude compared to the original spectrum. The presented method will benefit the analysis of quantification for the amplitude and frequency of supraharmonic emissions.
    Type of Medium: Online Resource
    ISSN: 1996-1073
    Language: English
    Publisher: MDPI AG
    Publication Date: 2021
    detail.hit.zdb_id: 2437446-5
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  • 8
    In: Agronomy, MDPI AG, Vol. 13, No. 4 ( 2023-04-18), p. 1152-
    Abstract: Ammannia auriculata Willd. (eared redstem) has become one of the most troublesome weeds in paddy rice in China. Resistance to bensulfuron-methyl (BSM) has spread extensively in this species. Greenhouse and field experiments were conducted to determine how the eared redstem biotype and density affect competition against rice. In the greenhouse experiment, five treatments were tested: a BSM-susceptible biotype at low density (58 plants m−2, SL), a BSM-susceptible biotype at high density (288 plants m−2, SH), a BSM-resistant biotype at low density (RL), a BSM-resistant biotype at high density (RH), and a control without eared redstem (CK). Eared redstem grew slowly until 15 days after sowing (DAS); however, growth accelerated after 20 DAS, and the eared redstem plants were taller than the rice from 55 DAS on. The SH and RH treatments were associated with greater intraspecific competition: eared redstem plants in the SH and RH treatments had fewer branches, fewer capsules, and less shoot dry weight per individual plant relative to the SL and RL treatments. The SH and RH treatments also caused greater reductions in the rice yield. The dry weight of rice at 141 DAS was reduced by 73% in the SL treatment, 98% in the SH treatment, 51% in the RL treatment, and 82% in the RH treatment, all relative to the CK. At 141 DAS, BSM-resistant plants were smaller than BSM-susceptible plants, suggesting a fitness cost of herbicide resistance in the absence of a herbicide. In the field study, eared redstem densities as low as 5 plants m−2 reduced the rice yield by 22%. A density of 50 eared redstem plants m−2 reduced the rice yield by 70%. Overall, these findings demonstrate that eared redstem is a highly aggressive weed species and threatens the rice yield even at a low density. However, the findings also demonstrate that BSM-resistant populations are less competitive. There is an urgent need to develop integrated management programs for this aggressive weed, which may include tactics to shift competitive dynamics in favor of rice. Additionally, this research provides the theoretical basis for the damage level, resistance risk evaluation, and management strategy of eared redstem in rice cropping systems.
    Type of Medium: Online Resource
    ISSN: 2073-4395
    Language: English
    Publisher: MDPI AG
    Publication Date: 2023
    detail.hit.zdb_id: 2607043-1
    SSG: 23
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  • 9
    In: Machines, MDPI AG, Vol. 10, No. 5 ( 2022-05-09), p. 350-
    Abstract: The oil-free lubrication seal of a piston rod plays an important role in the application of a Stirling engine. Parallel offset in a piston rod ruins the symmetry of the seal and affects the sealing performance when the seal is worn. In this paper, based on a motion analysis and the finite element method, a three-dimensional model of the Cap-seal was established, and its performance was numerically and experimentally investigated. The results show that parallel offset of the piston rod increases the possibility of seal damage and has no obvious effect on leakage. Under high pressures and low pre-compression ratios, the Cap-seal shows a good sealing capability and exhibits a higher propensity for mechanical damage. A good agreement was obtained between the numerical and experimental results. This study offers guidelines regarding the design and application of oil-free lubrication seals for a Stirling piston rod.
    Type of Medium: Online Resource
    ISSN: 2075-1702
    Language: English
    Publisher: MDPI AG
    Publication Date: 2022
    detail.hit.zdb_id: 2704328-9
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  • 10
    In: Fishes, MDPI AG, Vol. 7, No. 4 ( 2022-06-21), p. 142-
    Abstract: The red swamp crayfish (Procambarus clarkii) is an important aquatic animal and has developed as a popular aquaculture species in China. In this study, a total of 72,839 SSR motifs were identified from transcriptional data, and 20 microsatellite markers of them were finally developed to assess the genetic diversities of seven wild populations from natural lakes and nine cultured populations from rice fields. Genetic diversity was slightly higher in the cultured populations than in the wild populations. The degree of genetic differentiation between cultured populations is slight, while a moderate to a large degree of genetic differentiation between wild populations and most of the variations occurred within individuals (79%). The analysis of cluster, principal coordinate analysis and STRUCTURE were similar, and they showed that isolation-by-distance pattern was not significant. The microsatellite markers developed in this study can not only be used for genetic monitoring of population but also provide important information for the management of breeding and cultured population in red swamp crayfish.
    Type of Medium: Online Resource
    ISSN: 2410-3888
    Language: English
    Publisher: MDPI AG
    Publication Date: 2022
    detail.hit.zdb_id: 2932929-2
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