In:
Microbiology, Microbiology Society, Vol. 149, No. 10 ( 2003-10-01), p. 2733-2738
Kurzfassung:
Protein–protein interactions play an important role in all cellular processes. The development of two-hybrid systems in yeast and bacteria allows for in vivo assessment of such interactions. Using a recently developed bacterial two-hybrid system, the interactions of the Staphylococcus aureus proteins FemA, FemB and FmhB, members of the FemABX protein family, which is involved in peptidoglycan biosynthesis and β -lactam resistance of numerous Gram-positive bacteria, were analysed. While FmhB is involved in the addition of glycine 1 of the pentaglycine interpeptide of S. aureus peptidoglycan, FemA and FemB are specific for glycines 2/3 and 4/5, respectively. FemA–FemA, FemA–FemB and FemB–FemB interactions were found, while FmhB exists solely as a monomer. Interactions detected by the bacterial two-hybrid system were confirmed using the glutathione S -transferase-pulldown assay and gel filtration.
Materialart:
Online-Ressource
ISSN:
1350-0872
,
1465-2080
DOI:
10.1099/mic.0.26315-0
Sprache:
Englisch
Verlag:
Microbiology Society
Publikationsdatum:
2003
ZDB Id:
2008736-6
SSG:
12
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