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  • Ovid Technologies (Wolters Kluwer Health)  (12)
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  • Ovid Technologies (Wolters Kluwer Health)  (12)
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  • 1
    In: Medicine, Ovid Technologies (Wolters Kluwer Health), Vol. 99, No. 7 ( 2020-02), p. e17763-
    Kurzfassung: Unstable distal metaphyseal and dia-metaphyseal fractures of the radius may have treated with a variety of operative techniques, Kirschner wires (K-wires), dorsally inserted titanium elastic stable intramedullary nailing (DESIN), and short titanium elastic stable intramedullary nailing (SESIN) in children. The aim of this study was to evaluate the differences in clinical and radiographic outcomes between these methods. Between January 2009 and December 2017 196 children were treated for forearm fractures in the distal third of the distal radius. Gender of the patients, different types of surgical techniques, number of postoperative X-rays, date of metal removal and degree of axis deviation after the metal removal were studied. Distance of the fracture line from the radiocarpal surface, the width of the distal epiphysis of the radius, and the cumulative width of the distal epiphysis of the ulna and radius were analyzed. Out of the 196 children, stabilization of the fracture was achieved by K-wire in 139, by DESIN in 44, and by SESIN in 13 patients. The average time of metal removal was significantly shorter (3.8 months), following stabilization with K-wire. In children treated with K-wire, axial deviation of 〈 5° was seen in 118 patients, 5° to 10° deviation in 15 patients, while deviation was above 10° in 6 children. In the DESIN group, 〈 5° axial deviation was found in 37 patients and 5° to 10° in seven patients. In all 13 children treated with SESIN, axial deviation was measured to be 〈 5°. The fracture distance from the radiocarpal surface was on average 23.7 and 45.6 mm in the children treated with K-wire and DESIN, respectively. Fracture distance from the radiocarpal surface might determine the type of surgical technique required. If the distance of the fracture line is less than the width of the distal radius, osteosynthesis with a K-wire is recommended, while if the distance of the fracture is more than the cumulative width of the radius and the ulna, then DESIN may provide better results. The use of SESIN may be indicated when the area of the growth plate is injured.
    Materialart: Online-Ressource
    ISSN: 0025-7974 , 1536-5964
    Sprache: Englisch
    Verlag: Ovid Technologies (Wolters Kluwer Health)
    Publikationsdatum: 2020
    ZDB Id: 2049818-4
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 2
    Online-Ressource
    Online-Ressource
    Ovid Technologies (Wolters Kluwer Health) ; 2012
    In:  Journal of Urology Vol. 187, No. 3 ( 2012-03), p. 1110-1115
    In: Journal of Urology, Ovid Technologies (Wolters Kluwer Health), Vol. 187, No. 3 ( 2012-03), p. 1110-1115
    Materialart: Online-Ressource
    ISSN: 0022-5347 , 1527-3792
    RVK:
    Sprache: Englisch
    Verlag: Ovid Technologies (Wolters Kluwer Health)
    Publikationsdatum: 2012
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 3
    Online-Ressource
    Online-Ressource
    Ovid Technologies (Wolters Kluwer Health) ; 2011
    In:  International Clinical Psychopharmacology Vol. 26 ( 2011-09), p. e113-
    In: International Clinical Psychopharmacology, Ovid Technologies (Wolters Kluwer Health), Vol. 26 ( 2011-09), p. e113-
    Materialart: Online-Ressource
    ISSN: 0268-1315
    Sprache: Englisch
    Verlag: Ovid Technologies (Wolters Kluwer Health)
    Publikationsdatum: 2011
    ZDB Id: 1500677-3
    SSG: 15,3
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 4
    In: Shock, Ovid Technologies (Wolters Kluwer Health), Vol. 54, No. 1 ( 2020-07), p. 87-95
    Kurzfassung: The hypoxia-sensitive endothelin (ET) system plays an important role in circulatory regulation through vasoconstrictor ET A and ET B2 and vasodilator ET B1 receptors. Sepsis progression is associated with microcirculatory and mitochondrial disturbances along with tissue hypoxia. Our aim was to investigate the consequences of treatments with the ET A receptor (ET A -R) antagonist, ET B1 receptor (ET B1 -R) agonist, or their combination on oxygen dynamics, mesenteric microcirculation, and mitochondrial respiration in a rodent model of sepsis. Sprague Dawley rats were subjected to fecal peritonitis (0.6 g kg −1 i.p.) or a sham operation. Septic animals were treated with saline or the ET A -R antagonist ETR-p1/fl peptide (100 nmol kg −1 i.v.), the ET B1 -R agonist IRL-1620 (0.55 nmol kg −1 i.v.), or a combination therapy 22 h after induction. Invasive hemodynamic monitoring and blood gas analysis were performed during a 90-min observation, plasma ET-1 levels were determined, and intestinal capillary perfusion (CPR) was detected by intravital videomicroscopy. Mitochondrial Complex I (CI)- and CII-linked oxidative phosphorylation (OXPHOS) was evaluated by high-resolution respirometry in liver biopsies. Septic animals were hypotensive with elevated plasma ET-1. The ileal CPR, oxygen extraction (ExO 2 ), and CI–CII-linked OXPHOS capacities decreased. ETR-p1/fl treatment increased ExO 2 (by 〉 45%), CPR, and CII-linked OXPHOS capacity. The administration of IRL-1620 countervailed the sepsis-induced hypotension (by 〉 30%), normalized ExO 2 , and increased CPR. The combined ET A -R antagonist–ET B1 -R agonist therapy reduced the plasma ET-1 level, significantly improved the intestinal microcirculation (by 〉 41%), and reversed mitochondrial dysfunction. The additive effects of a combined ET A -R–ET B1 -R-targeted therapy may offer a tool for a novel microcirculatory and mitochondrial resuscitation strategy in experimental sepsis.
    Materialart: Online-Ressource
    ISSN: 1073-2322 , 1540-0514
    Sprache: Englisch
    Verlag: Ovid Technologies (Wolters Kluwer Health)
    Publikationsdatum: 2020
    ZDB Id: 2011863-6
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 5
    In: Arteriosclerosis, Thrombosis, and Vascular Biology, Ovid Technologies (Wolters Kluwer Health), Vol. 34, No. 4 ( 2014-04), p. 939-945
    Kurzfassung: Incorporation of novel plasma protein biomarkers may improve current models for prediction of atherosclerotic cardiovascular disease (ASCVD) risk. Approach and Results— We used discovery mass spectrometry (MS) to determine plasma concentrations of 861 proteins in 135 myocardial infarction (MI) cases and 135 matched controls. Then, we measured 59 markers by targeted MS in 336 ASCVD case–control pairs. Associations with MI or ASCVD were tested in single-marker and multiple-marker analyses adjusted for established ASCVD risk factors. Twelve single markers from discovery MS were associated with MI incidence (at P 〈 0.01), adjusting for clinical risk factors. Seven proteins in aggregate (cyclophilin A, cluster of differentiation 5 molecule [CD5] antigen-like, cell-surface glycoprotein mucin cell surface associated protein 18 [MUC-18] , collagen-α 1 [XVIII] chain, salivary α-amylase 1, C-reactive protein, and multimerin-2) were highly associated with MI ( P 〈 0.0001) and significantly improved its prediction compared with a model with clinical risk factors alone (C-statistic of 0.71 versus 0.84). Through targeted MS, 12 single proteins were predictors of ASCVD (at P 〈 0.05) after adjusting for established risk factors. In multiple-marker analyses, 4 proteins in combination (α-1–acid glycoprotein 1, paraoxonase 1, tetranectin, and CD5 antigen-like) predicted incident ASCVD ( P 〈 0.0001) and moderately improved the C-statistic from the model with clinical covariates alone (C-statistic of 0.69 versus 0.73). Conclusions— Proteomics profiling identified single- and multiple-marker protein panels that are associated with new-onset ASCVD and may lead to a better understanding of underlying disease mechanisms. Our findings include many novel protein biomarkers that, if externally validated, may improve risk assessment for MI and ASCVD.
    Materialart: Online-Ressource
    ISSN: 1079-5642 , 1524-4636
    Sprache: Englisch
    Verlag: Ovid Technologies (Wolters Kluwer Health)
    Publikationsdatum: 2014
    ZDB Id: 1494427-3
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 6
    In: Circulation, Ovid Technologies (Wolters Kluwer Health), Vol. 130, No. suppl_2 ( 2014-11-25)
    Kurzfassung: BACKGROUND: Metabolic syndrome is associated with increased risk for cardiovascular disease and type 2 diabetes, but the molecular mechanisms underpinning its constituent risk factors are unclear. We sought to identify predictive markers of metabolic risk by testing for associations of lipids and metabolites with longitudinal changes in metabolic traits. METHODS: Discovery liquid chromatography-tandem mass spectrometry profiling of 154 lipids and gas chromatography-mass spectrometry profiling of 119 metabolites was conducted on plasma samples from 554 Framingham Heart Study participants with baseline and follow-up clinic examinations (5-7 years later) at which body mass index (BMI), triglycerides (TG), HDL cholesterol (HDL-C), and glucose were measured. Analytes were tested for association with longitudinal changes (Δ) in metabolic traits using general linear models, and multimarker panels were selected using forward selection. RESULTS: Our single marker analyses revealed distinct signatures of longitudinal changes in metabolic risk factors. Markers associated with ΔBMI were 1-palmitoyl lysophosphatidic acid (LPA 16:0; p=2.8x10 -4 ), its precursor lysophosphatidylcholine (LPC 16:0; p=6.7x10 -5 ), and four other LPC species. Subclasses of sphingomyelins (SMs) were associated with changes in TG, HDL-C, and glucose. Sphingadiene (d18:2) variants were associated with ΔHDL-C [SM(d18:2/24:1), p=2.8x10 -6 ; SM (d18:2/20:0), p=1.2x10 -4 ]. Canonical SMs were associated with ΔTG [SM (d18:1/16:0), p=1.8x10 -5 ; SM (d18:1/17:0), p=2.1x10 -5 ]. Two dihydrosphingosine (d18:0) variants were associated with Δglucose [SM 36:0, p=5.0x10 -5 ; SM (d18:0/24:0), p=3.2x10 -4 ]. Metabolite markers for ΔTG included quinic acid (p=9.1x10 -5 ) and sitosterol (p=1.0x10 -4 ). Top markers were selected in multimarker panels that explained a significant proportion of longitudinal change in each metabolic trait (2.5-15.3%) beyond baseline covariates. CONCLUSIONS: Using lipidomic and metabolomic profiling in parallel, we identified lipid-centric signatures of longitudinal changes in metabolic traits and demonstrated their predictive power. Our results suggest that specific derangements in lipid metabolic pathways may underlie metabolic risk.
    Materialart: Online-Ressource
    ISSN: 0009-7322 , 1524-4539
    Sprache: Englisch
    Verlag: Ovid Technologies (Wolters Kluwer Health)
    Publikationsdatum: 2014
    ZDB Id: 1466401-X
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 7
    In: Arteriosclerosis, Thrombosis, and Vascular Biology, Ovid Technologies (Wolters Kluwer Health), Vol. 32, No. suppl_1 ( 2012-05)
    Kurzfassung: Background: Multiple cardiovascular disease (CVD) risk factors cluster in the same individuals and their concurrence is used to diagnose metabolic syndrome (MetSyn), which carries substantial risk for CVD. We hypothesized that MetSyn is associated with multiple metabolomic derangements. Methods: As part of the SABRe CVD initiative, a multi-project investigation of biomarkers of CVD and its risk factors, we designed a 2x2x2 factorial study of MetSyn risk factors that included 650 individuals from the Framingham Heart Study (out of a total N of ∼3200) assigned to 8 unique groups of approximately 81 individuals each, sampled from high vs. low strata of BMI, lipids, and glucose. We conducted gas chromatography-mass spectroscopy (GC/MS) on plasma samples from 650 eligible individuals. General linear modeling was used to identify biomarkers that differed across all 8 groups or differed in their main effects on individual risk factors. Results: Characteristics of the study sample (mean±SD), according to group assignment, are presented in the Table. GC/MS characterized 149 metabolites; of these 18 differed across all groups at P 〈 5x10 -8 and 36 differed at P 〈 0.00001. The top 3 most highly significant metabolites across all groups were glucose (P=2x10 -40 ), glutamic acid (P=4x10 -26 ), and sphingomyelins (lowest P=8x10 -25 ). The top 3 most highly significant main effects of metabolites for BMI were: glutamic acid (P=2x10 -18 ), sitosterol (P=2x10 -10 ), and uric acid (P=3x10 -10 ), for dyslipidemia: sphingomyelins (lowest P=1x10 -27 ), glutamic acid (P=5x10 -20 ), and lactic acid (P=7x10 -12 ), and for dysglycemia: glucose (P=1x10 -42 ), fructose (P=3x10 -7 ), and 2-hydroxybutanoic acid (P=6x10 -7 ). Conclusions: Metabolomic profiling identified multiple biomarker signatures of MetSyn and its major metabolic risk factors. These novel findings warrant external replication. Understanding the pathways represented by our results may help to unravel the molecular derangements contributing to MetSyn and its constituent risk factors. This knowledge may identify therapeutic targets for the prevention and treatment of CVD.
    Materialart: Online-Ressource
    ISSN: 1079-5642 , 1524-4636
    Sprache: Englisch
    Verlag: Ovid Technologies (Wolters Kluwer Health)
    Publikationsdatum: 2012
    ZDB Id: 1494427-3
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 8
    Online-Ressource
    Online-Ressource
    Ovid Technologies (Wolters Kluwer Health) ; 2023
    In:  Applied Immunohistochemistry & Molecular Morphology Vol. 31, No. 1 ( 2023-01), p. 26-32
    In: Applied Immunohistochemistry & Molecular Morphology, Ovid Technologies (Wolters Kluwer Health), Vol. 31, No. 1 ( 2023-01), p. 26-32
    Kurzfassung: Hypoxia and necrosis are common features of invasive cancer. The dynamic upregulation of carbonic anhydrase IX (CAIX), triggered by hypoxia-inducible factor 1 (HIF-1) is 1 of the mechanisms supporting cellular adaptation to hypoxia in solid tumors, including breast carcinoma. CAIX activity results in extracellular acidosis and in a profound reorganization of the tumor micro-environment, influencing biological behavior and prognosis. The main focus of our study was to evaluate the mass and distribution of the immune infiltrate, more specifically of CD8+ effector T-cells, in relation with tumoral CAIX expression. Materials and Methods: Formalin-fixed and paraffin-embedded breast carcinoma sections were analyzed following double immunohistochemical staining for CAIX and CD8. Scanned digital slides were evaluated for both labelings, and CD8-related signal was determined within and outside CAIX-positive tumor areas using the HistoQuant (3DHistech) image analysis software. Statistical analysis was performed using GraphPad Prism software. Results: Of the 34 breast carcinomas, 18 tested partially positive for CAIX. The remaining 16 cases were used as the CAIX-negative control group. Necrotic foci were generally associated with CAIX overexpression, and tumors exhibiting signs of necrosis had a significantly higher rate of relative CAIX expression compared with samples without necrosis (11.47±5.505 vs. without necrosis 3.765±3.5 P -value=0.0216). On the other hand, no statistically significant difference was found when comparing relative CD8+ lymphocyte counts in cases with necrosis as opposed to those where necrosis was absent (134.7±55.7 vs. 97.70±57.25; P value=0.1579). No difference in gross CD8+ T-lymphocyte infiltrate could be measured between CAIX positive and negative samples (98.48±37.32 vs. 95.99±50 P value=0.5928). However, in CAIX-expressing tumors a statistical correlation between the CD8+ T-lymphocyte infiltrate and the extent of CAIX-positive areas was observed. Within the same tumor, CD8+ T-lymphocyte counts showed a significant difference betweeen CAIX+ and CAIX- areas (13.06±9.4 vs. 135.6±62.2 P value 〈 0.0001). Conclusion: Our measurements demonstrate for the first time that tumor areas with CAIX expression potentially hamper CD8+ T-lymphocyte infiltration in breast carcinoma. The hypoxia-driven adaptive micro-environment likely interferes with the specific response to biological and immune therapies requiring intact effector T-cell response.
    Materialart: Online-Ressource
    ISSN: 1541-2016
    Sprache: Englisch
    Verlag: Ovid Technologies (Wolters Kluwer Health)
    Publikationsdatum: 2023
    ZDB Id: 2052398-1
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 9
    Online-Ressource
    Online-Ressource
    Ovid Technologies (Wolters Kluwer Health) ; 2006
    In:  Journal of Cardiovascular Pharmacology Vol. 47, No. 1 ( 2006-01), p. 103-109
    In: Journal of Cardiovascular Pharmacology, Ovid Technologies (Wolters Kluwer Health), Vol. 47, No. 1 ( 2006-01), p. 103-109
    Materialart: Online-Ressource
    ISSN: 0160-2446
    Sprache: Englisch
    Verlag: Ovid Technologies (Wolters Kluwer Health)
    Publikationsdatum: 2006
    ZDB Id: 2049700-3
    SSG: 15,3
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 10
    Online-Ressource
    Online-Ressource
    Ovid Technologies (Wolters Kluwer Health) ; 2018
    In:  Medicine Vol. 97, No. 13 ( 2018-03), p. e9991-
    In: Medicine, Ovid Technologies (Wolters Kluwer Health), Vol. 97, No. 13 ( 2018-03), p. e9991-
    Materialart: Online-Ressource
    ISSN: 0025-7974
    Sprache: Englisch
    Verlag: Ovid Technologies (Wolters Kluwer Health)
    Publikationsdatum: 2018
    ZDB Id: 2049818-4
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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