In:
PLOS ONE, Public Library of Science (PLoS), Vol. 16, No. 1 ( 2021-1-22), p. e0245614-
Abstract:
αKlotho is primarily known to express as a transmembrane protein. Proteolytic cleavage results in shedding of the extracellular domain which enters systemic circulation. A truncated form of αKlotho resulting from alternative splicing of the αKLOTHO transcript exists and is believed to be secreted, thereby also entering systemic circulation. Existing ELISA methods fail to distinguish between the two circulating isoforms resulting in inconsistencies in assessing circulating αKlotho levels. We have exploited a unique 15aa peptide sequence present in the alternatively spliced secreted isoform to generate an antibody and show that it is able to specifically detect only the secreted Klotho isoform in human plasma. This finding will facilitate in distinguishing the levels of different circulating Klotho isoforms in health and disease and enhance their potential to serve as a biomarker for CKD and other conditions.
Type of Medium:
Online Resource
ISSN:
1932-6203
DOI:
10.1371/journal.pone.0245614
DOI:
10.1371/journal.pone.0245614.g001
DOI:
10.1371/journal.pone.0245614.g002
DOI:
10.1371/journal.pone.0245614.s001
DOI:
10.1371/journal.pone.0245614.s002
DOI:
10.1371/journal.pone.0245614.s003
DOI:
10.1371/journal.pone.0245614.s004
DOI:
10.1371/journal.pone.0245614.s005
DOI:
10.1371/journal.pone.0245614.s006
DOI:
10.1371/journal.pone.0245614.r001
DOI:
10.1371/journal.pone.0245614.r002
DOI:
10.1371/journal.pone.0245614.r003
DOI:
10.1371/journal.pone.0245614.r004
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2021
detail.hit.zdb_id:
2267670-3
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