In:
Journal of Anatomy and Histopathology, VSMU N.N. Burdenko, Vol. 12, No. 3 ( 2023-10-06), p. 26-32
Abstract:
Individual resistance to hypoxia largely determines the severity of inflammatory processes and, probably, can affect the regeneration of the skin wound. The aim was to study reparative and inflammatory changes in the wound in Wistar rats with different resistance to hypoxia. Material and methods . The study was performed on mature male Wistar rats weighing 300–350 g. Before the experiment, the resistance of animals to hypoxia was determined. Rats were classified as highly resistant to hypoxia (HR), in which the time to take a lateral position in the pressure chamber “at a height” of 11,500 m was more than 360 s (n=5), to low-resistant (LR) – less than 40 s (n=5). A full-thickness wound with a diameter of 16 mm was applied to the animals in the interscapular zone of the back. On the 7th day after the infliction of the wound, a histological and morphometric study was performed. The proliferative activity of cells in the granulation tissue of the wound was determined immunohistochemically using the Ki-67 marker. Real-time PCR was used to evaluate the level of expression of the Hif-1α, Nf-κB, and Vegf genes. Results. It was shown that in rats with high resistance to hypoxia, the total number of cells and, among them, the number of inflammatory cells was higher than in rats with low resistance to hypoxia. However, the percentage of inflammatory cells in the compared groups did not differ significantly. Cell proliferative activity, as measured by the number of Ki-67 positive cells, was higher in rats with high resistance to hypoxia. In the granulation tissue of highly resistant to hypoxia rats, the expression of the Vegf gene was statistically significantly higher than in low-resistant animals, while there were no statistically significant differences in the level of expression of the Hif-1α, Nf-κB genes. Conclusion. In rats with high resistance to hypoxia, on the 7th day of healing of a full-thickness skin wound, reparative processes in it are more pronounced. In the horizontal layer of fibroblasts, there are more cells, among them inflammatory ones - histiocytes and macrophages, which is combined with a higher number of proliferating Ki-67 positive cells and the expression level of vascular endothelial growth factor (Vegf).
Type of Medium:
Online Resource
ISSN:
2225-7357
DOI:
10.18499/2225-7357-2023-12-3
DOI:
10.18499/2225-7357-2023-12-3-26-32
Language:
Unknown
Publisher:
VSMU N.N. Burdenko
Publication Date:
2023
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