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  • World Scientific Pub Co Pte Ltd  (5)
  • 1
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    #201 : Comparison of the Sensitivity of Detecting Cervical Bacteria with Next Generation Sequencing and Third Generation Sequencing Technologies
    World Scientific Pub Co Pte Ltd ; 2023
    In:  Fertility & Reproduction Vol. 05, No. 04 ( 2023-12), p. 632-634
    Details
    In: Fertility & Reproduction, World Scientific Pub Co Pte Ltd, Vol. 05, No. 04 ( 2023-12), p. 632-634
    Abstract: Background and Aims: In in vitro fertilization (IVF) cycles, some patients suffering with vaginosis showed poor reproductive outcome even transferring with good quality embryos. The aim of this pilot study was to evaluate whether the species of Lactobacillus could be detected by next generation sequencing (NGS) and Third generation sequencing (TGS). Method: 18 Patients aged 32–48 years-old who visited our fertility center from February 2021 to June 2022 with previous failure of transfer cycle were included. Exclusion criteria was antibiotic treatment within 3 months prior to enrolment. Genomic DNA of cervical microbiota taken from Cervico vaginal swabs in all patients were extracted and amplified. NGS was performed following the protocol of Ion 16S Metagenomics Kit by detecting V2–4–8 and V3–6, 7–9 regions of 16S. Amplicons were sequenced with Ion GeneStudio S5 Prime System. For TGS, full length of 16S was sequenced with Single Molecule, Real-Time (SMRT) Sequencing (Pacific Biosciences) and analyzed. Results: All of the 18 cervical samples could be amplified with V2–4–8 and V3–6, 7–9 primers and the genus could be 100% identified by NGS. However, most of the Lactobacillus includes L. crispatus, L. jensenii. and L. gasseri showed indistinguishable except of L. iners. On the other hand, TGS clearly identified all Lactobacillus. For Gardnerella, Atopobium and Prevotella, TGS and NGS showed equivalent sensitivity in genus level. However, due to the sequence similarity, Escherichia/Shigella could not be identified from the Lactobacillus. Conclusion: In this report, we aim to compare the sensitivity of detecting bacteria by 16S amplification method. Preliminary results suggest that NGS can distinguish bacterium causes vaginosis in genus level, but there might be misleading if the existence of pathogen such as Escherichia/Shigella. Till now, TGS still exhibits the best sensitivity for distinguish Lactobacillus in species level.
    Type of Medium: Online Resource
    ISSN: 2661-3182 , 2661-3174
    URL: Article
    DOI: 10.1142/S2661318223743631
    Language: English
    Publisher: World Scientific Pub Co Pte Ltd
    Publication Date: 2023
    detail.hit.zdb_id: 3051883-0
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  • 2
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    #202 : Adverse Effect on Pregnancy Outcome of Oocyte Preservation in Women with Advanced Maternal Age
    World Scientific Pub Co Pte Ltd ; 2023
    In:  Fertility & Reproduction Vol. 05, No. 04 ( 2023-12), p. 730-731
    Details
    In: Fertility & Reproduction, World Scientific Pub Co Pte Ltd, Vol. 05, No. 04 ( 2023-12), p. 730-731
    Abstract: Background and Aims: For women facing inevitable fertility decline, oocyte freezing is an effective method for fertility preservation. However, few studies discussed the pregnancy potential from autologous freezing oocytes. Thus, this study characterizes the realistic outcomes of advanced maternal age patients who underwent oocyte thawing. Method: In this retrospective cohort study, the selected 694 patients underwent oocyte freezing cycles (n=1,163) and oocyte thawing cycles (n=218) from Mar 2020 to Mar 2022 at our fertility center. All oocytes thaw cycles from Mar 2020 to Mar 2022 at TFC clinic, including social egg freezing (SEF) and egg collection (EC). Parameters for IVF laboratory outcome and pregnancy outcome were recorded, and statistics were analyzed with Chi-square. Results: Those patients who underwent egg freezing at average age for 40.2 years old, and 49.7% were over 37 years old. Secondly, the survival rate of oocyte thawing was 94.9% in patients under 38 years old, 93.7% were 38-40 years old, and 91.4% for patients over 41 years old. For patients under 38 years old, 72.2% of were conceived for thawing cycle. For advanced maternal age groups, 46.7% for patients in 38-40 years old group, and only 23.3% of patients over 41 years old were conceived. The live birth rate declines as the age increases, which were 55.6%, 33.3%, and 7.1% in different age groups. Conclusion: In this study, we found the survival rate of oocytes and blastulation of embryo showed no significant difference between different age groups. Clinical pregnancy outcome demonstrated a dramatic decline in patients aged over 41 years old compared with other groups. Therefore, these data suggested that oocyte collection strategy is more suitable for patients who are under 41 years old.
    Type of Medium: Online Resource
    ISSN: 2661-3182 , 2661-3174
    URL: Article
    DOI: 10.1142/S2661318223744363
    Language: English
    Publisher: World Scientific Pub Co Pte Ltd
    Publication Date: 2023
    detail.hit.zdb_id: 3051883-0
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  • 3
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    Automatic Brain Cancer Segmentation on PET Image
    World Scientific Pub Co Pte Ltd ; 2021
    In:  International Journal of Pattern Recognition and Artificial Intelligence Vol. 35, No. 03 ( 2021-03-15), p. 2150008-
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    In: International Journal of Pattern Recognition and Artificial Intelligence, World Scientific Pub Co Pte Ltd, Vol. 35, No. 03 ( 2021-03-15), p. 2150008-
    Abstract: When treating a brain tumor, a doctor needs to know the site and the size of the tumor. Positron emission tomography (PET) can be effectively applied to diagnose such cancers based on the heightened glucose metabolism of early-stage cancer cells. The purpose of this research is to extract the regions of skull, brain tumor, and brain tissue from a series of PET brain images and then a three-dimensional (3D) model is reconstructed from the extracted skulls, brain tumors, and brain tissues. Knowing the relative site and size of a tumor within the skull is helpful to a doctor. The contours obtained by the segmentation method proposed in this study are quantitatively compared with the contours drawn by doctors on the same image set since the ground truth is unknown. The experimental results are impressive.
    Type of Medium: Online Resource
    ISSN: 0218-0014 , 1793-6381
    URL: Article
    DOI: 10.1142/S0218001421500087
    Language: English
    Publisher: World Scientific Pub Co Pte Ltd
    Publication Date: 2021
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  • 4
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    #193 : Comparison of the Different Expression of OCT-4, GATA-3 and Clinical Pregnancy Outcome Between the Day 5 and Day 6 Blastocysts
    World Scientific Pub Co Pte Ltd ; 2023
    In:  Fertility & Reproduction Vol. 05, No. 04 ( 2023-12), p. 653-654
    Details
    In: Fertility & Reproduction, World Scientific Pub Co Pte Ltd, Vol. 05, No. 04 ( 2023-12), p. 653-654
    Abstract: Background and Aims: In common experience of in vitro-fertilization (IVF) cycle, blastocysts formed at Day 5 (D5) are regarded as higher quality and are more desirable for embryo transfer compared with those formed at Day 6 (D6). How those delayed D6 embryo differs from D5 embryo is less discussed. This study aimed to elucidate the cell content distinguished by immunofluorescent (IF) staining between the same grade of D5 and D6 blastocysts that might link to the clinical pregnancy outcome in frozen–thawed embryo from different day of blastocyst formation. Method: The retrospective study included 1327 frozen-thawed single blastocyst transfer (SBT) cycles in the Taipei Fertility Center during March 2020 to December 2022. All D5 (D5-SBT) and D6 (D6-SBT) frozen-thawed SET cycles (n=1327) were separated to non-PGS (n=671) and PGS-euploid (n=656) groups, then assigned to high grade (AA, AB, BA, BB) and low grade (BC, CB, CC). Aneuploidy and mosaicism embryo are excluded. For cell content analysis, pluripotent marker OCT-4 initially expressed in all blastomeres but restricted to the inner cell mass (ICM) of the blastocyst and downregulated in the trophectoderm (TE). GATA-3 is a TE specific marker which increases during pre-implantation embryo development. The cell number, OCT-4 and GATA-3 expression of same grade frozen-thawed D5 (n=14) and D6 (n=14) blastocysts were analyzed with IF staining. D5 extensively cultured to D6 (n=10) were used as the control group of regularly development. Cell numbers of TE and ICM region were examined by OCT-4 and GATA-3 IF detection. The total cell number was visualized with DAPI staining. Results: According to staining results, there were no significant differences in total cell number, TE and ICM cell number between the same grade D5 and D6 blastocysts. However, TE cell number significantly increased in D5 extensively cultured blastocysts, indicating higher proliferative capacity in TE cells from D5 blastocysts. In the clinical data of the non-PGS group, chemical pregnancy rate (HCG+) and clinical pregnancy rate (SAC+) of high grade D5-SBT were significantly increased compared with high grade D6-SBT (HCG+: 69.1% versus 47.0%, P 〈 0.001; SAC+: 63.0% versus 43.4%, P=0.001). In the PGS-euploid group, the chemical pregnancy rate (HCG+) showed significantly increased in high grade D5-SBT compared with high grade D6-SBT (69.5% versus 60.0%, P=0.047). The clinical pregnancy rate (SAC+) was significantly higher in high grade D5-SBT compared with high grade D6-SBT (65.5% versus 52.5%, P=0.009). The ongoing pregnancy rate was significantly higher in high grade D5-SBT compared with high grade D6-SBT (57.6% versus 43.3%, P=0.005). Conclusion: According to the IF data in this study, we demonstrated that the number of GATA3-positive TE cells were significantly decreased in D6 blastocyst compared with D5 extensively cultured blastocysts. Due to decreased proliferative capacity in high grade D6 blastocyst, transfer of high grade D5 blastocyst would exhibit higher ongoing pregnancy rate than D6 blastocyst.
    Type of Medium: Online Resource
    ISSN: 2661-3182 , 2661-3174
    URL: Article
    DOI: 10.1142/S2661318223743771
    Language: English
    Publisher: World Scientific Pub Co Pte Ltd
    Publication Date: 2023
    detail.hit.zdb_id: 3051883-0
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  • 5
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    #41 : The Effectiveness of GnRH Agonist Administration Prior to Frozen-Thawed Embryo Transfer with Artificial Cycle: A Systematic Review and Meta-Analysis of Different Protocols and Infertile Populations
    World Scientific Pub Co Pte Ltd ; 2023
    In:  Fertility & Reproduction Vol. 05, No. 04 ( 2023-12), p. 337-337
    Details
    In: Fertility & Reproduction, World Scientific Pub Co Pte Ltd, Vol. 05, No. 04 ( 2023-12), p. 337-337
    Abstract: Background and Aims: We investigated the use of GnRHa pretreatment with different protocols and durations of treatment on pregnancy outcomes in women undergoing FET with artificial cycles (AC-FET). Method: A systematic search of PubMed, EMBASE, Google Scholar, and Cochrane library was performed to include only eligible peer-reviewed studies in English published before September 01, 2022. This review was registered on PROSPERO (CRD42022299259) Results: A total of 41 studies (43021 individuals) were included with low to moderate risk of bias. Overall, GnRHa pretreatment improved clinical pregnancy rate (CPR) (OR=1.27, 95%CI:1.12-1.44), implantation rate (IR) (OR=1.24, 95%CI:1.07-1.45), live birth rate (LBR) (OR=1.31, 95%CI:1.07-1.60) and endometrial thickness (MD=0.16, 95%CI:0.03-0.30). Subgroup analyses of randomized and non-randomized studies both demonstrated the benefits of GnRHa pretreatment in CPR, IR, and LBR. Protocols using depot GnRHa (OR=1.19, 95%CI:1.04-1.36) showed a better LBR than daily GnRHa (OR=1.04, 95%CI:0.93-1.16). Women administered GnRHa for more than one cycle had better CPR (OR=2.01, 95%CI:1.29-3.13) and IR (OR=2.14, 95%CI:0.98-4.67) than those treated with GnRHa within one cycle before FET ([OR=1.18, 95%CI:1.04-1.33] and [OR=1.16, 95%CI:1.01-1.32] , respectively). In women with PCOS, pituitary suppression with GnRHa significantly improved CPR (OR=1.24, 95%CI:0.98-4.67), LBR (OR=1.22, 95%CI:1.05-1.42) and reduced the miscarriage rate (OR=0.75, 95%CI:0.59-0.95). In contrast, women with regular menstruation had a higher endometrial thickness (MD=0.16, 95%CI: 0.04-0.28) but no significant improvements in pregnancy outcomes. GnRHa pretreatment considerably improved IR (OR=2.21, 95%CI:1.60-3.07) in women with adenomyosis and CPR (OR=1.74, 95%CI:1.12-2.71), and LBR (OR=1.67, 95%CI:1.06-2.63) in women with endometriosis. Conclusion: GnRHa pretreatment, especially with longer suppression duration and the use of depot GnRHa, demonstrates a beneficial role during AC-FET cycles. Individuals with PCOS benefit from GnRHa pretreatment, though this FET protocol should be carefully considered in ovulatory women with regular menstruation. Albeit having the potential, effectiveness of this protocol on adenomyosis or endometriosis remains controversial and requires more rigorous investigation
    Type of Medium: Online Resource
    ISSN: 2661-3182 , 2661-3174
    URL: Article
    DOI: 10.1142/S2661318223741450
    Language: English
    Publisher: World Scientific Pub Co Pte Ltd
    Publication Date: 2023
    detail.hit.zdb_id: 3051883-0
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