In:
eLife, eLife Sciences Publications, Ltd, Vol. 6 ( 2017-08-14)
Abstract:
To determine the dynamics of allelic-specific expression during mouse development, we analyzed RNA-seq data from 23 F1 tissues from different developmental stages, including 19 female tissues allowing X chromosome inactivation (XCI) escapers to also be detected. We demonstrate that allelic expression arising from genetic or epigenetic differences is highly tissue-specific. We find that tissue-specific strain-biased gene expression may be regulated by tissue-specific enhancers or by post-transcriptional differences in stability between the alleles. We also find that escape from X-inactivation is tissue-specific, with leg muscle showing an unexpectedly high rate of XCI escapers. By surveying a range of tissues during development, and performing extensive validation, we are able to provide a high confidence list of mouse imprinted genes including 18 novel genes. This shows that cluster size varies dynamically during development and can be substantially larger than previously thought, with the Igf2r cluster extending over 10 Mb in placenta.
Type of Medium:
Online Resource
ISSN:
2050-084X
DOI:
10.7554/eLife.25125.001
DOI:
10.7554/eLife.25125.002
DOI:
10.7554/eLife.25125.003
DOI:
10.7554/eLife.25125.004
DOI:
10.7554/eLife.25125.005
DOI:
10.7554/eLife.25125.006
DOI:
10.7554/eLife.25125.007
DOI:
10.7554/eLife.25125.008
DOI:
10.7554/eLife.25125.009
DOI:
10.7554/eLife.25125.010
DOI:
10.7554/eLife.25125.011
DOI:
10.7554/eLife.25125.012
DOI:
10.7554/eLife.25125.013
DOI:
10.7554/eLife.25125.014
DOI:
10.7554/eLife.25125.015
DOI:
10.7554/eLife.25125.016
DOI:
10.7554/eLife.25125.017
DOI:
10.7554/eLife.25125.018
DOI:
10.7554/eLife.25125.023
DOI:
10.7554/eLife.25125.024
Language:
English
Publisher:
eLife Sciences Publications, Ltd
Publication Date:
2017
detail.hit.zdb_id:
2687154-3
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