In:
eLife, eLife Sciences Publications, Ltd, Vol. 6 ( 2017-10-23)
Abstract:
The ability to measure minute structural changes in neural circuits is essential for long-term in vivo imaging studies. Here, we propose a methodology for detection and measurement of structural changes in axonal boutons imaged with time-lapse two-photon laser scanning microscopy (2PLSM). Correlative 2PLSM and 3D electron microscopy (EM) analysis, performed in mouse barrel cortex, showed that the proposed method has low fractions of false positive/negative bouton detections (2/0 out of 18), and that 2PLSM-based bouton weights are correlated with their volumes measured in EM (r = 0.93). Next, the method was applied to a set of axons imaged in quick succession to characterize measurement uncertainty. The results were used to construct a statistical model in which bouton addition, elimination, and size changes are described probabilistically, rather than being treated as deterministic events. Finally, we demonstrate that the model can be used to quantify significant structural changes in boutons in long-term imaging experiments.
Type of Medium:
Online Resource
ISSN:
2050-084X
DOI:
10.7554/eLife.29315.001
DOI:
10.7554/eLife.29315.002
DOI:
10.7554/eLife.29315.003
DOI:
10.7554/eLife.29315.004
DOI:
10.7554/eLife.29315.005
DOI:
10.7554/eLife.29315.006
DOI:
10.7554/eLife.29315.007
DOI:
10.7554/eLife.29315.008
DOI:
10.7554/eLife.29315.009
DOI:
10.7554/eLife.29315.010
DOI:
10.7554/eLife.29315.011
DOI:
10.7554/eLife.29315.012
DOI:
10.7554/eLife.29315.013
DOI:
10.7554/eLife.29315.014
DOI:
10.7554/eLife.29315.017
DOI:
10.7554/eLife.29315.018
Language:
English
Publisher:
eLife Sciences Publications, Ltd
Publication Date:
2017
detail.hit.zdb_id:
2687154-3
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