In:
eLife, eLife Sciences Publications, Ltd, Vol. 10 ( 2021-05-18)
Abstract:
Hepatocellular carcinoma, the most common type of liver malignancy, is one of the most lethal forms of cancer. We identified a long non-coding RNA, Gm19705 , that is overexpressed in hepatocellular carcinoma and mouse embryonic stem cells. We named this RNA P luripotency and H epatocyte A ssociated R NA O verexpressed in H CC , or PHAROH . Depletion of PHAROH impacts cell proliferation and migration, which can be rescued by ectopic expression of PHAROH . RNA-seq analysis of PHAROH knockouts revealed that a large number of genes with decreased expression contain a Myc motif in their promoter. MYC is decreased in knockout cells at the protein level, but not the mRNA level. RNA-antisense pulldown identified nucleolysin TIAR, a translational repressor, to bind to a 71-nt hairpin within PHAROH , sequestration of which increases MYC translation. In summary, our data suggest that PHAROH regulates MYC translation by sequestering TIAR and as such represents a potentially exciting diagnostic or therapeutic target in hepatocellular carcinoma.
Type of Medium:
Online Resource
ISSN:
2050-084X
Language:
English
Publisher:
eLife Sciences Publications, Ltd
Publication Date:
2021
detail.hit.zdb_id:
2687154-3
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