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  • General works  (3)
  • 1
    Online Resource
    Online Resource
    The Royal Society ; 2014
    In:  Philosophical Transactions of the Royal Society A: Mathematical, Physical and Engineering Sciences Vol. 372, No. 2030 ( 2014-12-13), p. 20140203-
    In: Philosophical Transactions of the Royal Society A: Mathematical, Physical and Engineering Sciences, The Royal Society, Vol. 372, No. 2030 ( 2014-12-13), p. 20140203-
    Abstract: A prototype instrument, under development at the University of Leicester, for the future European Space Agency (ESA) ExoMars mission, was used for the analysis of microbial pigments within a stratified gypsum crust from a hypersaline saltern evaporation pond at Eilat (Israel). Additionally, the same samples were analysed using a miniaturized Raman spectrometer, featuring the same 532 nm excitation. The differences in the position of the specific bands, attributed to carotenoid pigments from different coloured layers, were minor when analysed by the ESA prototype instrument; therefore, making it difficult to distinguish among the different pigments. The portable Delta Nu Advantage instrument allowed for the discrimination of microbial carotenoids from the orange/green and purple layers. The purpose of this study was to complement previous laboratory results with new data and experience with portable or handheld Raman systems, even with a dedicated prototype Raman system for the exploration of Mars. The latter is equipped with an excitation wavelength falling within the carotenoid polyene resonance region. The ESA prototype Raman instrument detected the carotenoid pigments (biomarkers) with ease, although further detailed distinctions among them were not achieved.
    Type of Medium: Online Resource
    ISSN: 1364-503X , 1471-2962
    RVK:
    Language: English
    Publisher: The Royal Society
    Publication Date: 2014
    detail.hit.zdb_id: 208381-4
    detail.hit.zdb_id: 1462626-3
    SSG: 11
    SSG: 5,1
    SSG: 5,21
    Library Location Call Number Volume/Issue/Year Availability
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  • 2
    Online Resource
    Online Resource
    The Royal Society ; 2014
    In:  Philosophical Transactions of the Royal Society A: Mathematical, Physical and Engineering Sciences Vol. 372, No. 2030 ( 2014-12-13), p. 20140194-
    In: Philosophical Transactions of the Royal Society A: Mathematical, Physical and Engineering Sciences, The Royal Society, Vol. 372, No. 2030 ( 2014-12-13), p. 20140194-
    Abstract: Salts are abundant on Mars, and any liquid water that is present or may have been present on the planet is expected to be hypersaline. Halophilic archaea (family Halobacteriaceae) are the microorganisms best adapted to life at extremes of salinity on Earth. This paper reviews the properties of the Halobacteriaceae that may make the group good candidates for life also on Mars. Many species resist high UV and gamma radiation levels; one species has survived exposure to vacuum and radiation during a space flight; and there is at least one psychrotolerant species. Halophilic archaea may survive for millions of years within brine inclusions in salt crystals. Many species have different modes of anaerobic metabolism, and some can use light as an energy source using the light-driven proton pump bacteriorhodopsin. They are also highly tolerant to perchlorate, recently shown to be present in Martian soils, and some species can even use perchlorate as an electron acceptor to support anaerobic growth. The presence of characteristic carotenoid pigments ( α -bacterioruberin and derivatives) makes the Halobacteriaceae easy to identify by Raman spectroscopy. Thus, if present on Mars, such organisms may be detected by Raman instrumentation planned to explore Mars during the upcoming ExoMars mission.
    Type of Medium: Online Resource
    ISSN: 1364-503X , 1471-2962
    RVK:
    Language: English
    Publisher: The Royal Society
    Publication Date: 2014
    detail.hit.zdb_id: 208381-4
    detail.hit.zdb_id: 1462626-3
    SSG: 11
    SSG: 5,1
    SSG: 5,21
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 3
    Online Resource
    Online Resource
    The Royal Society ; 2014
    In:  Philosophical Transactions of the Royal Society A: Mathematical, Physical and Engineering Sciences Vol. 372, No. 2030 ( 2014-12-13), p. 20140199-
    In: Philosophical Transactions of the Royal Society A: Mathematical, Physical and Engineering Sciences, The Royal Society, Vol. 372, No. 2030 ( 2014-12-13), p. 20140199-
    Abstract: In this paper, it is demonstrated how Raman spectroscopy can be used to detect different carotenoids as possible biomarkers in various groups of microorganisms. The question which arose from previous studies concerns the level of unambiguity of discriminating carotenoids using common Raman microspectrometers. A series of laboratory-grown microorganisms of different taxonomic affiliation was investigated, such as halophilic heterotrophic bacteria, cyanobacteria, the anoxygenic phototrophs, the non-halophilic heterotrophs as well as eukaryotes (Ochrophyta, Rhodophyta and Chlorophyta). The data presented show that Raman spectroscopy is a suitable tool to assess the presence of carotenoids of these organisms in cultures. Comparison is made with the high-performance liquid chromatography approach of analysing pigments in extracts. Direct measurements on cultures provide fast and reliable identification of the pigments. Some of the carotenoids studied are proposed as tracers for halophiles, in contrast with others which can be considered as biomarkers of other genera. The limits of application of Raman spectroscopy are discussed for a few cases where the current Raman spectroscopic approach does not allow discriminating structurally very similar carotenoids. The database reported can be used for applications in geobiology and exobiology for the detection of pigment signals in natural settings.
    Type of Medium: Online Resource
    ISSN: 1364-503X , 1471-2962
    RVK:
    Language: English
    Publisher: The Royal Society
    Publication Date: 2014
    detail.hit.zdb_id: 208381-4
    detail.hit.zdb_id: 1462626-3
    SSG: 11
    SSG: 5,1
    SSG: 5,21
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
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