In:
Plant, Cell & Environment, Wiley, Vol. 46, No. 4 ( 2023-04), p. 1195-1206
Abstract:
Leaf‐form ferredoxin‐NADP + oxidoreductases (LFNRs) play an important role in regulating ferredoxin (FD)‐dependent electron partitioning in the chloroplast, acting at the branch of photosynthetic electron transfer and reductive metabolism. However, the membrane‐anchoring mechanism of LFNRs and its physiological roles in rice remains largely elusive. Here, we compared the growth phenotype, fresh weight, chlorophyll content, photosynthetic rate, and electron transfer rate among the wild‐type (WT), Oslfnr1‐1, Oslfnr2‐1, Ostic62, Ostrol1‐1 mutants, and Ostrol1‐1 and Ostic62 double mutant. The results showed that mutation of OsLFNRs and their putative anchor partners affects plant growth and photosynthesis. We found that OsTIC62 is necessary for thylakoid‐bound high‐molecular‐weight LFNR complexes, as low‐molecular‐weight LFNR complexes on OsTROL1 and medium‐molecular‐weight complexes are likely a mixture of OsTROL2‒OsLFNR and OsTIC62‒OsLFNR. OsLFNR tightly bind to the thylakoid membrane through OsTIC62 and OsTROL1, this binding stabilizes OsLFNR; also, OsLFNR and its membrane anchors reciprocally stabilize each other. What is more, loss of OsTROL1 leads to a compensatory increase in OsTIC62. In Oslfnr2 mutants, we could not find any OsLFNR complexes in the thylakoid membrane, whereas the Oslfnr1 mutant still had many OsLFNR complexes, which suggested that OsLFNR2 is required for the binding of OsLFNRs to the thylakoid membrane. As the expression pattern of membrane anchors under diurnal conditions has not been reported, we investigated the expression pattern of OsTIC62 under diurnal condition. Gene expression and protein levels suggested that OsTIC62 is highly expressed under dark than in daylight at both the transcript and protein levels, which means that OsTIC62 expression have day‒night rhythm.
Type of Medium:
Online Resource
ISSN:
0140-7791
,
1365-3040
Language:
English
Publisher:
Wiley
Publication Date:
2023
detail.hit.zdb_id:
391893-2
detail.hit.zdb_id:
2020843-1
SSG:
12
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