In:
Applied Spectroscopy, SAGE Publications, Vol. 41, No. 3 ( 1987-03), p. 503-508
Abstract:
Total internal reflection fluorescence spectroscopy (TIRF) is an established technique for following the course of interfacial reactions. Theoretically, by gathering TIRF data as a function of observation angle, one can obtain the density of fluorophores with respect to distance away from a solid/liquid interface. In order that the practical application of the theory might be explored, variable observation angle data from solutions of fluorescein and from adsorbed layers of fluorescein isothio-cyanate labeled immunoglobulin have been analyzed in terms of simple concentration-distance profiles. In all cases the general shape of the data curves was found to conform to the theoretical expectation. Layer thickness determinations varied over a range of 20 to 100 nm, with concentrations in the layer ranging from 12 to 61 mg/mL. The theoretical background, sources of error, and system improvements are also discussed.
Type of Medium:
Online Resource
ISSN:
0003-7028
,
1943-3530
DOI:
10.1366/0003702874448913
Language:
English
Publisher:
SAGE Publications
Publication Date:
1987
detail.hit.zdb_id:
1474251-2
SSG:
11
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