In:
The Journal of the Acoustical Society of America, Acoustical Society of America (ASA), Vol. 122, No. 5_Supplement ( 2007-11-01), p. 3052-3052
Abstract:
Echogenic liposomes (ELIP), phospholipid vesicles filled with gas and fluid, can be manufactured to incorporate the thrombolytic drug tissue plasminogen activator (tPA). Real-time thrombolysis of blood clots exposed to tPA-incorporating ELIP (t-ELIP) was monitored using video microscopy with an inverted optical microscope. Human whole blood clots on silk sutures were exposed to tPA alone (3.15 micrograms/ml), t-ELIP alone (3.15 micrograms/ml), t-ELIP and 120 kHz ultrasound (0.18 MPa peak negative pressure, 1.667 kHz pulse repetition frequency, 50% duty cycle), or tPA and ultrasound, for 30 min. The extent of thrombolysis was determined by assessing clot width as a function of time, using a time-lapse microscopic imaging technique. The average percent change in clot width at 30 min for clots treated with t-ELIP alone exceeded tPA alone (22.8% vs. 15.6%, respectively). Thrombolytic efficacy was similar for either tPA or t-ELIP exposed to 120 kHz ultrasound. Thus, the thrombolytic drug could be effectively released by exposure to 120 kHz ultrasound. [This work was supported by The Distinguished Chair for Clinical Research in Emergency Medicine Foundation Award, K02-NS056253, NIH 1RO1 NS047603, and NIH 1R01 HL074002.]
Type of Medium:
Online Resource
ISSN:
0001-4966
,
1520-8524
Language:
English
Publisher:
Acoustical Society of America (ASA)
Publication Date:
2007
detail.hit.zdb_id:
1461063-2
Bookmarklink