In:
Frontiers in Microbiology, Frontiers Media SA, Vol. 13 ( 2022-4-18)
Kurzfassung:
Daptomycin is a cyclic lipopeptide antibiotic with a significant antibacterial action against antibiotic-resistant Gram-positive bacteria. Despite numerous attempts to enhance daptomycin yield throughout the years, the production remains unsatisfactory. This study reports the application of multilevel metabolic engineering strategies in Streptomyces roseosporus to reconstruct high-quality daptomycin overproducing strain L2797-VHb, including precursor engineering (i.e., refactoring kynurenine pathway), regulatory pathway reconstruction (i.e., knocking out negative regulatory genes arpA and phaR ), byproduct engineering (i.e., removing pigment), multicopy biosynthetic gene cluster (BGC), and fermentation process engineering (i.e., enhancing O 2 supply). The daptomycin titer of L2797-VHb arrived at 113 mg/l with 565% higher comparing the starting strain L2790 (17 mg/l) in shake flasks and was further increased to 786 mg/l in 15 L fermenter. This multilevel metabolic engineering method not only effectively increases daptomycin production, but can also be applied to enhance antibiotic production in other industrial strains.
Materialart:
Online-Ressource
ISSN:
1664-302X
DOI:
10.3389/fmicb.2022.872397
DOI:
10.3389/fmicb.2022.872397.s001
DOI:
10.3389/fmicb.2022.872397.s002
DOI:
10.3389/fmicb.2022.872397.s003
DOI:
10.3389/fmicb.2022.872397.s004
DOI:
10.3389/fmicb.2022.872397.s005
DOI:
10.3389/fmicb.2022.872397.s006
DOI:
10.3389/fmicb.2022.872397.s007
DOI:
10.3389/fmicb.2022.872397.s008
DOI:
10.3389/fmicb.2022.872397.s009
DOI:
10.3389/fmicb.2022.872397.s010
DOI:
10.3389/fmicb.2022.872397.s011
DOI:
10.3389/fmicb.2022.872397.s012
DOI:
10.3389/fmicb.2022.872397.s013
Sprache:
Unbekannt
Verlag:
Frontiers Media SA
Publikationsdatum:
2022
ZDB Id:
2587354-4
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