In:
European Journal of Endocrinology, Oxford University Press (OUP), Vol. 131, No. 1 ( 1994-07), p. 91-96
Kurzfassung:
Amenta F, Chiandussi L, Mancini M, Ricci A, Schena M, Veglio F. Pharmacological characterization and autoradiographic localization of dopamine receptors in the human adrenal cortex. Eur J Endocrinol 1994;131:91–6. ISSN 0804–4643 The pharmacological characteristics and the anatomical localization of dopamine D 1 -like and D 2 -like receptors were studied in sections of the human adrenal cortex using radioligand binding and autoradiographic techniques. [ 3 H]SCH23390 was used as a ligand of D 1 -like receptors, whereas [ 3 H]spiroperidol was used to label D 2 -like receptors. No specific [ 3 H]SCH 23390 binding was detectable in sections of the human adrenal cortex. On the other hand, [ 3 H]spiroperidol was bound to sections of the adrenal gland in a manner consistent with the labelling of dopamine D 2 -like receptor sites. The binding was time, temperature and concentration dependent, belonging in the range of concentrations of the radioligand used for a single class of high-affinity sites. The dissociation constant (K d ) averaged 2.7 nmol/l, whereas the maximum density of binding sites (B max ) was 160 nmol/mg tissue. Experiments on the pharmacological specificity of [ 3 H]spiroperidol binding to sections of the human adrenal cortex revealed that clozapine was the most powerful displacer of [ 3 H]spiroperidol from sections of the human adrenal cortex. This suggests the presence in the human adrenal cortex of dopamine receptors of the D 4 subtype. Light microscope autoradiography showed the highest density of specific [ 3 H]spiroperidol binding sites in the zona glomerulosa and to a lesser extent in the zona reticularis. Only sparse [ 3 H]spiroperidol binding sites were localized in the zona fasciculata. The possible functional consequences of this localization of dopamine D 2 -like receptor sites in the human adrenal cortex are discussed. Francesco Amenta, Istituto di Farmacologia, Via Scalzino 5, 62032 Camerino, Italy
Materialart:
Online-Ressource
ISSN:
0804-4643
,
1479-683X
DOI:
10.1530/eje.0.1310091
Sprache:
Unbekannt
Verlag:
Oxford University Press (OUP)
Publikationsdatum:
1994
ZDB Id:
1485160-X
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