In:
Frontiers in Nutrition, Frontiers Media SA, Vol. 9 ( 2022-2-23)
Kurzfassung:
The diet-induced gut microbiota dysbiosis has been suggested as a major risk factor for atherothrombosis, however, the detailed mechanism linking these conditions is yet to be fully understood. Methods We established a long-term excessive-energy diet-induced metabolic syndrome (MetS) inbred Wuzhishan minipig model, which is characterized by its genetic stability, small size, and human-like physiology. The metabolic parameters, atherosclerotic lesions, gut microbiome, and host transcriptome were analyzed. Metabolomics profiling revealed a linkage between gut microbiota and atherothrombosis. Results We showed that white atheromatous plaque was clearly visible on abdominal aorta in the MetS model. Furthermore, using metagenome and metatranscriptome sequencing, we discovered that the long-term excessive energy intake altered the local intestinal microbiota composition and transcriptional profile, which was most dramatically illustrated by the reduced abundance of SCFAs-producing bacteria including Bacteroides, Lachnospiraceae, and Ruminococcaceae in the MetS model. Liver and abdominal aorta transcriptomes in the MetS model indicate that the diet-induced gut microbiota dysbiosis activated host chronic inflammatory responses and significantly upregulated the expression of genes related to arachidonic acid-dependent signaling pathways. Notably, metabolomics profiling further revealed an intimate linkage between arachidonic acid metabolism and atherothrombosis in the host-gut microbial metabolism axis. Conclusions These findings provide new insights into the relationship between atherothrombosis and regulation of gut microbiota via host metabolomes and will be of potential value for the treatment of cardiovascular diseases in MetS.
Materialart:
Online-Ressource
ISSN:
2296-861X
DOI:
10.3389/fnut.2022.807118
DOI:
10.3389/fnut.2022.807118.s001
DOI:
10.3389/fnut.2022.807118.s002
DOI:
10.3389/fnut.2022.807118.s003
DOI:
10.3389/fnut.2022.807118.s004
DOI:
10.3389/fnut.2022.807118.s005
DOI:
10.3389/fnut.2022.807118.s006
DOI:
10.3389/fnut.2022.807118.s007
DOI:
10.3389/fnut.2022.807118.s008
DOI:
10.3389/fnut.2022.807118.s009
Sprache:
Unbekannt
Verlag:
Frontiers Media SA
Publikationsdatum:
2022
ZDB Id:
2776676-7
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