In:
Cytometry Part B: Clinical Cytometry, Wiley, Vol. 70B, No. 3 ( 2006-05), p. 170-178
Abstract:
Determining T‐cell phenotypes of lung cells obtained by bronchoalveolar lavage (BAL) is frequently clinically useful, particularly for evaluating causes of interstitial lung disease. The current standard of determining CD4/CD8 T‐cell subsets by immunohistochemical (IHC) staining of cytocentrifuge slides is labor‐intensive and subject to interpreter variation. Flow cytometry (FCM) is a precise and rapid method commonly used in research to characterize cells in the lung. However, few studies address the methodology of analysis of BAL lymphocytes by FCM. Methods: Patients underwent bronchoscopy for clinical purposes. A BAL cell differential and T‐cell subtype was requested by the treating physician to supplement the evaluation of patients with suspected interstitial lung disease. We used a commercially available T‐cell antibody reagent, approved for analysis of blood via FCM, for T‐cell subtyping of clinical BAL specimens. Results: The percentages of CD4 and CD8 T‐cell populations, as well as the CD4/CD8 ratios showed excellent correlation with IHC staining of cytocentrifuge slides regardless of the acquisition program used, as long as the gating strategy remained consistent ( r ≥ 0.9693 for CD4, r ≥ 0.9589 for CD8, and r ≥ 0.9485 for the CD4/CD8 ratio). Conclusion: These findings validate the use of standardized, commercially available antibody cocktails for BAL lymphocyte subtyping, making this technique available to clinicians and researchers with access to a three‐color or four‐color flow cytometer. © 2006 International Society for Analytical Cytology
Type of Medium:
Online Resource
ISSN:
1552-4949
,
1552-4957
DOI:
10.1002/cyto.b.v70b:3
DOI:
10.1002/cyto.b.20101
Language:
English
Publisher:
Wiley
Publication Date:
2006
detail.hit.zdb_id:
2180651-2
SSG:
12
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