In:
International Journal of Life Sciences, Nepal Journals Online (JOL), Vol. 1 ( 1970-01-01), p. 6-10
Abstract:
Glucose isomerase (EC 5.3.1.5) was extracted from Streptomyces spp., isolated from Mt. Everest soil sample, and purified by ammonium sulfate fractionation and Sepharose-4B chromatography. A 7.1 fold increase in specific activity of the purified enzyme over crude was observed. Using glucose as substrate, the Michaelis constant (KM 〈 ) and maximal velocity (Vmax) were found to be 0.45M and 0.18U/mg. respectively. The optimum substrate (glucose) concentration, optimum enzyme concentration, optimum pH, optimum temperature, and optimum reaction time were 0.6M, 62.14μg/100μl, 6.9, 70ºC, and 30 minutes, respectively. Optimum concentrations of Mg2+ and Co2+ were 5mM and 0.5mM, respectively. The enzyme was thermostable with half-life 30 minutes at 100ºC.DOI: 10.3126/ijls.v1i0.2300 Int J Life Sci 1 : 6-10
Type of Medium:
Online Resource
ISSN:
2091-0525
DOI:
10.3126/ijls.v1i0.2300
Language:
Unknown
Publisher:
Nepal Journals Online (JOL)
Publication Date:
1970
detail.hit.zdb_id:
2722047-3
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