In:
FEBS Letters, Wiley, Vol. 410, No. 2-3 ( 1997-06-30), p. 407-412
Abstract:
Kynurenine 3‐monooxygenase, an NADPH‐dependent flavin monooxygenase, catalyses the hydroxylation of l ‐kynurenine to l ‐3‐hydroxykynurenine. By hybridization screening using a cDNA probe encoding the entire exon 2 of Drosophila melanogaster kynurenine 3‐monooxygenase, we isolated a 2.0 kb cDNA clone coding for the corresponding human liver enzyme. The deduced amino acid sequence of the human protein consists of 486 amino acids with a predicted molecular mass of 55 762 Da. Transfection of the human cDNA in HEK‐293 cells resulted in the functional expression of the enzyme with kinetic properties similar to those found for the native human protein. RNA blot analysis of human tissues revealed the presence of a major mRNA species of ∼2.0 kb in liver, placenta and kidney.
Type of Medium:
Online Resource
ISSN:
0014-5793
,
1873-3468
DOI:
10.1016/S0014-5793(97)00627-3
Language:
English
Publisher:
Wiley
Publication Date:
1997
detail.hit.zdb_id:
1460391-3
SSG:
12
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