In:
PLOS Biology, Public Library of Science (PLoS), Vol. 19, No. 6 ( 2021-6-18), p. e3001265-
Abstract:
The search for potential antibody-based diagnostics, vaccines, and therapeutics for pandemic severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has focused almost exclusively on the spike (S) and nucleocapsid (N) proteins. Coronavirus membrane (M), ORF3a, and ORF8 proteins are humoral immunogens in other coronaviruses (CoVs) but remain largely uninvestigated for SARS-CoV-2. Here, we use ultradense peptide microarray mapping to show that SARS-CoV-2 infection induces robust antibody responses to epitopes throughout the SARS-CoV-2 proteome, particularly in M, in which 1 epitope achieved excellent diagnostic accuracy. We map 79 B cell epitopes throughout the SARS-CoV-2 proteome and demonstrate that antibodies that develop in response to SARS-CoV-2 infection bind homologous peptide sequences in the 6 other known human CoVs. We also confirm reactivity against 4 of our top-ranking epitopes by enzyme-linked immunosorbent assay (ELISA). Illness severity correlated with increased reactivity to 9 SARS-CoV-2 epitopes in S, M, N, and ORF3a in our population. Our results demonstrate previously unknown, highly reactive B cell epitopes throughout the full proteome of SARS-CoV-2 and other CoV proteins.
Type of Medium:
Online Resource
ISSN:
1545-7885
DOI:
10.1371/journal.pbio.3001265
DOI:
10.1371/journal.pbio.3001265.g001
DOI:
10.1371/journal.pbio.3001265.g002
DOI:
10.1371/journal.pbio.3001265.g003
DOI:
10.1371/journal.pbio.3001265.g004
DOI:
10.1371/journal.pbio.3001265.g005
DOI:
10.1371/journal.pbio.3001265.g006
DOI:
10.1371/journal.pbio.3001265.g007
DOI:
10.1371/journal.pbio.3001265.t001
DOI:
10.1371/journal.pbio.3001265.t002
DOI:
10.1371/journal.pbio.3001265.s001
DOI:
10.1371/journal.pbio.3001265.s002
DOI:
10.1371/journal.pbio.3001265.s003
DOI:
10.1371/journal.pbio.3001265.s004
DOI:
10.1371/journal.pbio.3001265.s005
DOI:
10.1371/journal.pbio.3001265.s006
DOI:
10.1371/journal.pbio.3001265.s007
DOI:
10.1371/journal.pbio.3001265.s008
DOI:
10.1371/journal.pbio.3001265.s009
DOI:
10.1371/journal.pbio.3001265.s010
DOI:
10.1371/journal.pbio.3001265.s011
DOI:
10.1371/journal.pbio.3001265.s012
DOI:
10.1371/journal.pbio.3001265.s013
DOI:
10.1371/journal.pbio.3001265.r001
DOI:
10.1371/journal.pbio.3001265.r002
DOI:
10.1371/journal.pbio.3001265.r003
DOI:
10.1371/journal.pbio.3001265.r004
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2021
detail.hit.zdb_id:
2126773-X
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