In:
Skin Research and Technology, Wiley, Vol. 14, No. 3 ( 2008-08), p. 304-311
Abstract:
Background: Oxygen‐dependent quenching of luminescence using transparent planar sensor foils was shown to overcome the limitations of the polarographic electrode technique in an animal model. This method was then transferred to a clinical setting to measure the transcutaneous pO 2 (p tc O 2 ). Methods: In six healthy subjects, a cuff on the upper arm was occluded up to 20 mmHg above systolic pressure and released after 8 min. P tc O 2 was measured at the lower arm every 30 s before, during, and up to 20 min after cuff occlusion (40 °C applied skin temperature) using luminescence lifetime imaging (LLI) of platinum(II)‐octaethyl‐porphyrin immobilized in a polystyrene matrix. For validation, the polarographic Clark electrode technique was applied in close proximity, and measurements were conducted simultaneously. Results: P tc O 2 measurements before (70.8±19.1 vs. 66.2±7.7 mmHg) and at the end of ischemic (2.7±1.2 vs. 3.6±1.7 mmHg) and reperfusion phases (72.2±3.6 vs. 68.4±8.9 mmHg) did not differ significantly using the Clark electrode vs. LLI. At both the initial ischemic and the reperfusion phases, the Clark electrode measured a faster decrease or increase, respectively, in p tc O 2 because of the oxygen consumption occurring in this method. Conclusion: The presented method provides accurate and reproducible p tc O 2 values under changing microcirculatory conditions. The lack of oxygen consumption during measurement allows both a more realistic estimation of p tc O 2 than compared with the gold standard and permanent use in regions with critical oxygen supply.
Type of Medium:
Online Resource
ISSN:
0909-752X
,
1600-0846
DOI:
10.1111/srt.2008.14.issue-3
DOI:
10.1111/j.1600-0846.2008.00295.x
Language:
English
Publisher:
Wiley
Publication Date:
2008
detail.hit.zdb_id:
2025540-8
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