In:
Digestive Diseases, S. Karger AG, Vol. 40, No. 2 ( 2022), p. 223-231
Abstract:
〈 b 〉 〈 i 〉 Background: 〈 /i 〉 〈 /b 〉 Early detection of liver cirrhosis is crucial for secondary prevention of complications. However, noninvasive blood-based patient monitoring tools are lacking. In this explorative study, we conducted a targeted metabolomic analysis in order to identify possible serum markers indicating alcoholic liver cirrhosis (aLiC) with or without hepatocellular carcinoma (HCC). 〈 b 〉 〈 i 〉 Methods: 〈 /i 〉 〈 /b 〉 Venous blood of 30 individuals was collected: healthy controls (“Con”, 〈 i 〉 n 〈 /i 〉 = 12), patients with aLiC without and with HCC (“aLiC”: 〈 i 〉 n 〈 /i 〉 = 6 and “aLiC + HCC”: 〈 i 〉 n 〈 /i 〉 = 6), and patients with other liver diseases (“oLiD”: 〈 i 〉 n 〈 /i 〉 = 6). A targeted metabolomic analysis was conducted using the AbsoluteIDQ 〈 sup 〉 ® 〈 /sup 〉 p180 Kit (Biocrates Life Sciences 〈 sup 〉 ® 〈 /sup 〉 , Innsbruck, Austria). Statistical analysis was performed by applying a one-way ANOVA on all subgroups followed by a 〈 i 〉 t 〈 /i 〉 test for pairwise comparison of subgroups and logistic regression analysis. 〈 b 〉 〈 i 〉 Results: 〈 /i 〉 〈 /b 〉 ANOVA revealed 29 metabolites that significantly discriminate between the different cohorts. Among these analytes, 25 were significantly altered in Con versus aLiC, as indicated by 〈 i 〉 t 〈 /i 〉 test, most importantly SM C18:1 ( 〈 i 〉 p 〈 /i 〉 & #x3c; 0.001), SM C20:2 ( 〈 i 〉 p 〈 /i 〉 = 0.001), SM (OH) C22:2 ( 〈 i 〉 p 〈 /i 〉 & #x3c; 0.001), lysoPC a C20:4 ( 〈 i 〉 p 〈 /i 〉 & #x3c; 0.001), and PC aa C36:5 ( 〈 i 〉 p 〈 /i 〉 & #x3c; 0.001). To a similar extent, the metabolites discriminated also between the oLiD and aLiC but less between the Con or oLiD and aLiC + HCC cohorts. Most of these analytes were either lyso- and phosphatidylcholines or sphingomyelins. Results were not significant for comparison of Con versus oLiD and aLiC versus aLiC + HCC. 〈 b 〉 〈 i 〉 Conclusion: 〈 /i 〉 〈 /b 〉 Decreased lyso- and phosphatidylcholine as well as sphingomyelin species in venous blood could help to detect liver cirrhosis in patients with non-cirrhotic liver disease.
Type of Medium:
Online Resource
ISSN:
0257-2753
,
1421-9875
Language:
English
Publisher:
S. Karger AG
Publication Date:
2022
detail.hit.zdb_id:
1482221-0
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