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  • 1
    Online Resource
    Online Resource
    Hematological reference values for stray colony cats of northern Italy : Hematological references intervals for stray cats
    Springer Science and Business Media LLC ; 2016
    In:  Comparative Clinical Pathology Vol. 25, No. 4 ( 2016-7), p. 903-910
    Details
    In: Comparative Clinical Pathology, Springer Science and Business Media LLC, Vol. 25, No. 4 ( 2016-7), p. 903-910
    Type of Medium: Online Resource
    ISSN: 1618-5641 , 1618-565X
    URL: Article
    DOI: 10.1007/s00580-016-2280-7
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2016
    detail.hit.zdb_id: 1472425-X
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  • 2
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    Feline Leishmania spp. Infection in a Non-Endemic Area of Northern Italy
    MDPI AG ; 2020
    In:  Animals Vol. 10, No. 5 ( 2020-05-08), p. 817-
    Details
    In: Animals, MDPI AG, Vol. 10, No. 5 ( 2020-05-08), p. 817-
    Abstract: Feline leishmaniosis (FeL) is an emerging vector-borne feline disease, with increasing numbers of cases reported and studies performed internationally. This study aimed to update the epidemiological status for FeL in stray cats in Milan, northern Italy; compare these results with previous studies in Northern Italy; and report clinicopathologic findings and coinfections in cats infected with Leishmania spp. A total of 117 cats were tested for L. infantum and retrovirus infection, hematological, and biochemical parameters. Demographic and clinical data were collected and FeL affected cats screened for selected coinfections. Overall, 10/117 (8.6%) cats tested positive for L. infantum: in five cats L. infantum DNA was found in popliteal lymph nodes and five were IFAT seropositive at titers from 1:80 to 1:160. Infected cats were concentrated in a specific area of Milan (p = 0.0154). No specific clinicopathologic abnormalities or retroviral infections were significantly linked to the infection, other than hypergammaglobulinemia (p = 0.0127). Seroreactivity to Anaplasma phagocytophilum, Chlamydophila felis, and Toxoplasma gondii was found in some infected cats. A high prevalence of FeL was found in a non-endemic area of northern Italy and future studies should continually monitor this data to understand whether these cases are imported or if Leishmania vectors are present in this area.
    Type of Medium: Online Resource
    ISSN: 2076-2615
    URL: Article
    DOI: 10.3390/ani10050817
    Language: English
    Publisher: MDPI AG
    Publication Date: 2020
    detail.hit.zdb_id: 2606558-7
    SSG: 23
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  • 3
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    Prevalence of Ca Blood Type and Alloantibodies in a Population of Horses from Italy
    MDPI AG ; 2020
    In:  Animals Vol. 10, No. 7 ( 2020-07-13), p. 1179-
    Details
    In: Animals, MDPI AG, Vol. 10, No. 7 ( 2020-07-13), p. 1179-
    Abstract: A knowledge of the blood groups and alloantibodies present is essential for the safe transfusion of blood products in horses. Pre-transfusion screening and blood typing minimizes the risk of incompatible RBC transfusions and prevents immunization of the recipient against incompatible RBC antigens. The frequencies of blood groups can vary among different breeds. Knowledge of a breed’s blood group prevalence can be very useful for identifying the best blood donors during transfusion in clinical practice. The aims of this study were to estimate the prevalence of the Ca blood type in horses from Italy using a monoclonal immunocromatographic method and to estimate the prevalence of anti-Ca alloantibodies in Ca− horses using agglutination on gel technique. Ca blood type was determined on 110 whole blood samples. The prevalence of the Ca+ blood type was 79.1%. This study also provides data about the prevalence of Ca+ blood group in Italian Saddle Horses (77,3%) and Dutch Warmblood (58,3%). No significant association was found between Ca blood type and sex with 79.5% and 78.8% of females and males testing Ca+, respectively. The total number of Ca− samples with detectable anti-Ca alloantibodies was 7/23 (30.4%).
    Type of Medium: Online Resource
    ISSN: 2076-2615
    URL: Article
    DOI: 10.3390/ani10071179
    Language: English
    Publisher: MDPI AG
    Publication Date: 2020
    detail.hit.zdb_id: 2606558-7
    SSG: 23
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  • 4
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    Online Resource
    Hematological and Biochemical Reference Values in Healthy Captive Tigers (Panthera tigris)
    MDPI AG ; 2021
    In:  Animals Vol. 11, No. 12 ( 2021-12-02), p. 3440-
    Details
    In: Animals, MDPI AG, Vol. 11, No. 12 ( 2021-12-02), p. 3440-
    Abstract: The tiger (Panthera tigris) is an endangered species. The health of individuals is important and any data on hematological and biochemical blood values can provide valuable information; when combined with physical assessment. This data assists in both the diagnosis of disease and some conservation strategies. The behavior of wild tigers makes it is extremely difficult to obtain biological samples from free-living subjects, therefore, data collected from captive tigers is highly valuable. The aim of this study was to provide additional information for the values of hematological and serum biochemical parameters in healthy captive tigers. Blood samples were collected from 22 clinically healthy tigers (Panthera tigris). The following parameters were analyzed: glucose, urea, creatinine, alanine aminotransferase (ALT), alkaline phosphatase (ALP), total protein (TP) and red blood cells (RBCs), hemoglobin (Hb), hematocrit (Hct) and red cell indices; such as mean cell volume (MCV), mean cell Hb (MCH), mean cell Hb concentration (MCHC), platelet (PLT) and white blood cells (WBCs). The mean hematological values in our tiger population were not significantly different when compared with the same parameters in the previously studied tiger population. The mean values of RBCs and PLT were statistically significantly higher and the mean values of Hb, PCV, MCV, MCH, MCHC, and WBC were lower than the mean values obtained in previous studies on the Amur tiger. Further investigation of captive and free-living tigers is needed to identify the normal ranges for parameters in this endangered species.
    Type of Medium: Online Resource
    ISSN: 2076-2615
    URL: Article
    DOI: 10.3390/ani11123440
    Language: English
    Publisher: MDPI AG
    Publication Date: 2021
    detail.hit.zdb_id: 2606558-7
    SSG: 23
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  • 5
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    Online Resource
    Serum Protein Gel Agarose Electrophoresis in Captive Tigers
    MDPI AG ; 2020
    In:  Animals Vol. 10, No. 4 ( 2020-04-20), p. 716-
    Details
    In: Animals, MDPI AG, Vol. 10, No. 4 ( 2020-04-20), p. 716-
    Abstract: Given the endangered status of tigers (Panthera tigris), the health of each individual is important and any data on blood chemistry values can provide valuable information alongside the assessment of physical condition. The nature of tigers in the wild makes it is extremely difficult to obtain biological samples from free-living subjects, therefore the values obtained from captive tigers provide very useful data. Serum protein electrophoresis is a useful tool in the diagnosis and monitoring of a number of diseases. In this study, we evaluated agarose gel serum protein electrophoresis on samples from 11 healthy captive tigers. Serum electrophoresis on all 11 tiger samples successfully separated proteins into albumin, α1, α2, β1, β2 and γ globulin fractions as in other mammals. Electrophoretic patterns were comparable in all tigers. Mean± standard deviation or median and range values obtained for each protein fraction in healthy tigers were, respectively: 3.6 ± 0.2, 0.21 (0.2–0.23), 1.2 ± 0.2, 10.7 ± 0.2, 0.4 (0.3–0.6), 1.2 (1–1.8) gr/dL. The results of this preliminary study provide the first data on serum electrophoretic patterns in tigers and may be a useful diagnostic tool in the health assessment of this endangered species.
    Type of Medium: Online Resource
    ISSN: 2076-2615
    URL: Article
    DOI: 10.3390/ani10040716
    Language: English
    Publisher: MDPI AG
    Publication Date: 2020
    detail.hit.zdb_id: 2606558-7
    SSG: 23
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  • 6
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    Evaluation of Association between Blood Phenotypes A, B and AB and Feline Coronavirus Infection in Cats
    MDPI AG ; 2022
    In:  Pathogens Vol. 11, No. 8 ( 2022-08-15), p. 917-
    Details
    In: Pathogens, MDPI AG, Vol. 11, No. 8 ( 2022-08-15), p. 917-
    Abstract: Cats are susceptible to feline coronavirus (FCoV), a highly contagious virus with fecal–oral transmission. In people, susceptibility to coronavirus infection, such as SARS-CoV infection, has been associated with the ABO blood group, with individuals with blood group O having significantly lower risk of SARS-CoV infection. This study evaluated a possible association between feline blood group phenotypes A, B and AB and serostatus for antibodies against FCoV. We also investigated risk or protective factors associated with seropositivity for FCoV in the investigated population. Feline populations were surveyed for AB group system blood types and for presence of antibodies against FCoV. Blood phenotype, origin, breed, gender, reproductive status and age of cats were evaluated as protective or risk factors for coronavirus infection. No blood type was associated with FCoV seropositivity, for which being a colony stray cat (p = 0.0002, OR = 0.2, 95% CI: 0.14–0.54) or a domestic shorthair cat (p = 0.0075, OR = 0.2, 95% CI = 0.09–0.69) were protective factors. Based on results of this study, feline blood phenotypes A, B or AB do not seem to predispose cats to seropositivity for FCoV. Future studies on other feline blood types and other infections could clarify whether feline blood types could play a role in predisposing to, or protecting against, feline infections.
    Type of Medium: Online Resource
    ISSN: 2076-0817
    URL: Article
    DOI: 10.3390/pathogens11080917
    Language: English
    Publisher: MDPI AG
    Publication Date: 2022
    detail.hit.zdb_id: 2695572-6
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  • 7
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    Online Resource
    Prevalence of dog erythrocyte antigens 1, 4, and 7 in galgos (Spanish Greyhounds)
    SAGE Publications ; 2015
    In:  Journal of Veterinary Diagnostic Investigation Vol. 27, No. 4 ( 2015-07), p. 558-561
    Details
    In: Journal of Veterinary Diagnostic Investigation, SAGE Publications, Vol. 27, No. 4 ( 2015-07), p. 558-561
    Abstract: Galgos (Spanish Greyhounds), in common with other sighthounds, have higher hematocrits, hemoglobin concentrations, and red blood cell counts than other breeds. In addition to these hematological characteristics, the physical characteristics of these dogs (medium to large dogs with an easily accessible jugular vein and a good temperament) make galgos ideal blood donors. However, to date, there are only published reports concerning dog erythrocyte antigen (DEA) 1 in this breed. Information on DEAs 4 and 7 would be useful when recruiting blood donors to donation programs, as DEA 1 and 7–negative and DEA 4–positive dogs can be considered universal donors. Ethylenediamine tetra-acetic acid–anticoagulated jugular blood samples were collected from 205 galgos. Dogs were aged between 1 and 10 years, 102 were female (49.8%) and 103 male (50.2%), and all were living in South Madrid, Spain. All 205 blood samples were tested for DEA 1 by card agglutination, and 150 of these samples were tested for DEA 4 and DEA 7 by gel column agglutination using polyclonal anti-DEA antibodies. Of the 205 galgos blood samples typed, 112 out of 205 (54.6%) were positive for DEA 1. Of the 150 blood samples tested, all (150/150, 100%) were positive for DEA 4, and 12 out of 150 (8%) samples tested positive for DEA 7. Of these samples, 70 out of 150 (46.7%) were positive only for DEA 4. There was no relationship between blood types and sex. In addition to the hematological characteristics previously reported and the physical characteristics of these dogs, the relative prevalence of blood types DEA 1, 4, and 7 make galgos good candidates for blood donation in blood donor programs.
    Type of Medium: Online Resource
    ISSN: 1040-6387 , 1943-4936
    URL: Article
    DOI: 10.1177/1040638715592025
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2015
    detail.hit.zdb_id: 2265211-5
    SSG: 22
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  • 8
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    Online Resource
    Activity, specificity, and titer of naturally occurring canine anti–DEA 7 antibodies
    SAGE Publications ; 2016
    In:  Journal of Veterinary Diagnostic Investigation Vol. 28, No. 6 ( 2016-11), p. 705-708
    Details
    In: Journal of Veterinary Diagnostic Investigation, SAGE Publications, Vol. 28, No. 6 ( 2016-11), p. 705-708
    Abstract: The reported prevalence of naturally occurring anti–dog erythrocyte antigen (DEA) 7 antibodies in DEA 7–negative dogs is as high as 50%. Characterization of these antibodies may better define their importance in canine transfusion medicine. We determined in vitro activity, specificity, and titer of anti–DEA 7 antibodies in DEA 7–negative dogs. Plasma samples from 317 DEA 7–negative dogs were cross-matched with DEA 7–positive red blood cells (RBCs) using gel column technology. Agglutination occurred with DEA 7–positive RBCs but not with DEA 7–negative RBCs in 73 samples (23%), which were hence classified as containing anti–DEA 7 antibodies. These samples were evaluated for hemolytic and agglutinating activity, strength of agglutination, and antibody specificity and titers. All samples showed agglutination but none showed hemolysis. Gel agglutination was graded as 1+ for 20 samples (27%), 2+ for 49 samples (67%), 3+ for 4 samples (6%); no samples were graded 4+. The agglutination titer was 〈 1:2 for 51 samples (73%), 1:2 for 13 samples (19%), 1:4 for 4 samples (5%), and 1:8 for 2 samples (3%). Of 16 samples treated with 2-mercaptoethanol, 11 samples (69%) contained only IgM, 4 samples (25%) exhibited only IgG activity, and 1 sample (6%) had both IgG and IgM activity. Low titers of warm, weakly agglutinating, mostly naturally occurring IgM anti–DEA 7 antibodies were found in 23% of DEA 7–negative dogs. The presence of naturally occurring anti–DEA 7 antibodies suggests that cross-matching of canine blood recipients is advisable, even at first transfusion, to minimize delayed transfusion reactions.
    Type of Medium: Online Resource
    ISSN: 1040-6387 , 1943-4936
    URL: Article
    DOI: 10.1177/1040638716668626
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2016
    detail.hit.zdb_id: 2265211-5
    SSG: 22
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  • 9
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    Online Resource
    Prevalence of Leishmania infantum and co-infections in stray cats in northern Italy
    Elsevier BV ; 2016
    In:  Comparative Immunology, Microbiology and Infectious Diseases Vol. 45 ( 2016-04), p. 53-58
    Details
    In: Comparative Immunology, Microbiology and Infectious Diseases, Elsevier BV, Vol. 45 ( 2016-04), p. 53-58
    Type of Medium: Online Resource
    ISSN: 0147-9571
    URL: Article
    DOI: 10.1016/j.cimid.2016.03.001
    Language: English
    Publisher: Elsevier BV
    Publication Date: 2016
    detail.hit.zdb_id: 2025439-8
    SSG: 12
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  • 10
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    Online Resource
    Haematological and morphological evaluation of feline whole blood units collected for transfusion purposes
    SAGE Publications ; 2019
    In:  Journal of Feline Medicine and Surgery Vol. 21, No. 8 ( 2019-08), p. 732-740
    Details
    In: Journal of Feline Medicine and Surgery, SAGE Publications, Vol. 21, No. 8 ( 2019-08), p. 732-740
    Abstract: Despite the increasing availability of feline blood collected and stored for transfusion purposes, few studies have been performed on feline blood units. The aim of this prospective in vitro study was to evaluate haematological and morphological changes in feline blood cells in whole blood units between collection and end of storage. Methods Haematological examination (red blood cells [RBCs], haemoglobin, haematocrit, red cell distribution width, mean cell volume, mean cell haemoglobin concentration, mean cell haemoglobin, white blood cells [WBCs] and platelet [PLT] count) was performed on 40 non-leukoreduced feline whole blood units at the time of collection (day[D] 0) and after storage (D35). The blood was collected into citrate–phosphate–dextrose–adenine anticoagulant-preservative solution using an open system in a veterinary blood bank and stored for 35 days at 4 ± 2°C. Twenty of these feline whole blood units were also analysed for blood cell morphology (normal RBCs, macrocytes, echinocytes, spherocytes, schistocytes, lysed RBCs, RBCs with Heinz bodies and recognisable WBC and PLT count). Differences between the two examination times were statistically analysed. Results There was a statistically significant decrease in WBC and PLT counts after storage at D35 ( P 〈 0.0001 for both). The most significant cellular morphological changes after storage were an increase in echinocyte count ( P = 0.0001), and lysed RBCs ( P 〈 0.0001), and a decrease in normal RBCs ( P 〈 0.0001). Recognisable WBCs – mainly lymphocytes – were present at the end of storage. Conclusions and relevance This study showed that significant morphological changes occur in RBCs in feline blood units during storage for 35 days. In vivo studies are required to establish if these changes could affect the ability of stored RBCs to circulate and provide adequate oxygen delivery after transfusion.
    Type of Medium: Online Resource
    ISSN: 1098-612X , 1532-2750
    URL: Article
    DOI: 10.1177/1098612X18798841
    Language: English
    Publisher: SAGE Publications
    Publication Date: 2019
    detail.hit.zdb_id: 2049047-1
    SSG: 22
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