In:
Eukaryotic Cell, American Society for Microbiology, Vol. 1, No. 6 ( 2002-12), p. 1021-1031
Abstract:
In Saccharomyces cerevisiae , the actin cytoskeleton is depolarized by NaCl stress. In this study, the response was maximal after 30 min, and then actin patches repolarized. Rvs161p was required for actin repolarization because the rvs161Δ mutant did not repolarize actin patches after growth in a salt medium. Mutations suppressing the rvs161Δ -related salt sensitivity all occurred in genes required for sphingolipid biosynthesis: FEN1 , SUR4 , SUR2 , SUR1 , and IPT1 . These suppressors also suppressed act1-1 -related salt sensitivity and the defect in actin repolarization of the rvs161Δ mutant, providing a link between sphingolipids and actin polarization. Indeed, deletion of the suppressor genes suppressed the rvs161Δ defect in actin repolarization in two ways: either actin was not depolarized at the wild-type level in a set of suppressor mutants, or actin was repolarized in the absence of Rvs161p in the other suppressor mutants. Rvs161p was localized as cortical patches that concentrated at polarization sites, i.e., bud emergence and septa, and was found to be associated with lipid rafts. An important link between sphingolipids and actin polarization is that Rvs161p was required for actin repolarization and was found to be located in lipid rafts.
Type of Medium:
Online Resource
ISSN:
1535-9778
,
1535-9786
DOI:
10.1128/EC.1.6.1021-1031.2002
Language:
English
Publisher:
American Society for Microbiology
Publication Date:
2002
detail.hit.zdb_id:
2071564-X
SSG:
12
Bookmarklink