In:
Journal of Cellular Physiology, Wiley, Vol. 202, No. 3 ( 2005-03), p. 831-838
Abstract:
The high number ( 〉 10 8–10 ) of primary human pro‐erythroblasts (CD36 high /CD235a low ) obtainable in HEMA culture (Migliaccio et al., 2002 ) is exploited here to analyse the expression of proteins implicated in erythropoietin (EPO)‐signalling (STATs, PI‐3K, and PLCs) during the process of erythroid maturation. Human pro‐erythroblasts progressed in 4 days of culture with EPO into basophilic‐ (CD36 high /CD235a medium , 24 h), polychromatic‐(CD36 high /CD235a high , 48 h), and, finally, orthochromatic‐(CD36 low /CD235a high , 72–96 h) erythroblasts. During this maturation, STAT‐1 was expressed up to the orthochromatic stage, expression of STAT‐5, as well as of its target proteins Bcl xL and IRF 1 , remained constant up to 48 h (polychromatic‐erythroblasts) but decreased by 96 h (orthochromatic‐erythroblasts), while that of STAT‐3 decreased constantly from 24 h on and became undetectable by 96 h. Expression of PI‐3K rapidly decreased with differentiation since only 50% of original protein levels were detected by 48 h. On the other hand, among the members of PLC families investigated, PLC β 4 was not expressed, PLC β 2 , δ 1 , and γ 2 were expressed at constant levels throughout the maturation process, while expression of PLC β 3 and of PLC γ 1 decreased, as PI‐3K, by 24 h and that of PLC β 1 was induced by 6 h and became undetectable by 24 h. In conclusion, these data depict the dynamic signalling scenario associated with the maturation of erythroid cells and provide the first indication that members of PLC families (PLC β 1 , β 3 , and γ 1 ) might be involved in the control of erythroid differentiation in humans. © 2004 Wiley‐Liss, Inc.
Type of Medium:
Online Resource
ISSN:
0021-9541
,
1097-4652
Language:
English
Publisher:
Wiley
Publication Date:
2005
detail.hit.zdb_id:
1478143-8
SSG:
12
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