In:
Proceedings of the National Academy of Sciences, Proceedings of the National Academy of Sciences, Vol. 98, No. 17 ( 2001-08-14), p. 9942-9947
Abstract:
Royal College of Surgeons rats are genetically predisposed to
undergo significant visual loss caused by a primary dysfunction of retinal pigment epithelial (RPE) cells. By using this model, we have
examined the efficacy of subretinal transplantation of two independent human RPE cell lines each exhibiting genetic modifications that confer
long-term stability in vitro . The two cell lines, a
spontaneously derived cell line (ARPE19) and an extensively characterized genetically engineered human RPE cell line (h1RPE7),
which expresses SV40 large T (tumor) antigen, were evaluated separately. Both lines result in a significant preservation of visual
function as assessed by either behavioral or physiological techniques. This attenuation of visual loss correlates with photoreceptor survival
and the presence of donor cells in the areas of rescued photoreceptors at 5 months postgrafting (6 months of age). These results demonstrate
the potential of genetically modified human RPE cells for ultimate application in therapeutic transplantation strategies for retinal
degenerative diseases caused by RPE dysfunction.
Type of Medium:
Online Resource
ISSN:
0027-8424
,
1091-6490
DOI:
10.1073/pnas.171266298
Language:
English
Publisher:
Proceedings of the National Academy of Sciences
Publication Date:
2001
detail.hit.zdb_id:
209104-5
detail.hit.zdb_id:
1461794-8
SSG:
11
SSG:
12
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