In:
Analytical and Bioanalytical Chemistry, Springer Science and Business Media LLC, Vol. 413, No. 29 ( 2021-12), p. 7277-7294
Abstract:
Structural determination of N -glycans by mass spectrometry is ideally performed by negative ion collision-induced dissociation because the spectra are dominated by cross-ring fragments leading to ions that reveal structural details not available by many other methods. Most glycans form [M – H] - or [M + adduct] - ions but larger ones (above approx. m/z 2000) typically form doubly charged ions. Differences have been reported between the fragmentation of singly and doubly charged ions but a detailed comparison does not appear to have been reported. In addition to [M + adduct] - ions (this paper uses phosphate as the adduct) other doubly, triply, and quadruply charged ions of composition [M n + (H 2 PO 4 ) n ] n - have been observed in mixtures of N -glycans released from viral and other glycoproteins. This paper explores the formation and fragmentation of these different types of multiply charged ions with particular reference to the presence of diagnostic fragments in the CID spectra and comments on how these ions can be used to characterize these glycans. Graphical abstract
Type of Medium:
Online Resource
ISSN:
1618-2642
,
1618-2650
DOI:
10.1007/s00216-021-03480-8
Language:
English
Publisher:
Springer Science and Business Media LLC
Publication Date:
2021
detail.hit.zdb_id:
1459122-4
detail.hit.zdb_id:
2071767-2
SSG:
12
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