In:
Fetal Diagnosis and Therapy, S. Karger AG, Vol. 45, No. 5 ( 2019), p. 302-311
Abstract:
〈 b 〉 〈 i 〉 Objectives: 〈 /i 〉 〈 /b 〉 To evaluate the failure rate and performance of cell-free DNA (cfDNA) testing, mainly in terms of detection rates for trisomy 21, performed by 2 laboratories using different analytical methods. 〈 b 〉 〈 i 〉 Methods: 〈 /i 〉 〈 /b 〉 cfDNA testing was performed on 2,870 pregnancies with the Harmony 〈 sup 〉 TM 〈 /sup 〉 Prenatal Test using the targeted digital analysis of selected regions (DANSR) method, and on 2,635 pregnancies with the “Cerba test” using the genome-wide massively parallel sequencing (GW-MPS) method, with available outcomes. Propensity score analysis was used to match patients between the 2 groups. A comparison of the detection rates for trisomy 21 between the 2 laboratories was made. 〈 b 〉 〈 i 〉 Results: 〈 /i 〉 〈 /b 〉 In all, 2,811 patients in the Harmony group and 2,530 patients in the Cerba group had no trisomy 21, 18, or 13. Postmatched comparisons of the patient characteristics indicated a higher no-result rate in the Harmony group (1.30%) than in the Cerba group (0.75%; 〈 i 〉 p 〈 /i 〉 = 0.039). All 41 cases of trisomy 21 in the Harmony group and 93 cases in the Cerba group were detected. 〈 b 〉 〈 i 〉 Conclusions: 〈 /i 〉 〈 /b 〉 Both methods of cfDNA testing showed low no-result rates and a comparable performance in detecting trisomy 21; yet GW-MPS had a slightly lower no-result rate than the DANSR method.
Type of Medium:
Online Resource
ISSN:
1015-3837
,
1421-9964
Language:
English
Publisher:
S. Karger AG
Publication Date:
2019
detail.hit.zdb_id:
1482292-1
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