In:
Zeitschrift für Naturforschung C, Walter de Gruyter GmbH, Vol. 31, No. 3-4 ( 1976-4-1), p. 174-178
Abstract:
The sedimentation at low speed centrifugation of a lipoprotein recombined from the lipids, and the strongly bound proteins of the human erythrocyte membrane depends on pH : between 4.5 and 6.0, most of the liproprotein sediments, whereas at pH 7.0 - 8.5, up to 90% remains in the supernatant. Precipitation of the lipoprotein can be reversed by increasing the pH, followed by a brief sonication. The mobility of spin-labelled protein groups in the lipoprotein increases with increasing pH. This mobility increase is also reversible and is of equal magnitude in precipitated and nonprecipitated recombinates. It is concluded that, because of these reversibilities, determination of the yield of liproprotein formation in recombination experiments must include analysis of both precipitated and nonprecipitated lipoproteins.
Type of Medium:
Online Resource
ISSN:
1865-7125
,
0939-5075
DOI:
10.1515/znc-1976-3-415
Language:
English
Publisher:
Walter de Gruyter GmbH
Publication Date:
1976
detail.hit.zdb_id:
2078107-6
SSG:
12
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