In:
eLife, eLife Sciences Publications, Ltd, Vol. 2 ( 2013-10-01)
Abstract:
All eukaryotic cells contain an organelle called the Golgi apparatus, which consists of a series of four to six flattened structures called cisternae. Proteins that are intended for secretion from the cell, or proteins that go on to become part of the cell membrane, must pass through the Golgi, where they undergo modifications that ensure they are targeted to the correct place. There are two main models for how proteins are transported from the entry side of the Golgi, known as the cis face, to the exit side (trans face), through a process known as anterograde transport. One possibility is that the cargo protein matures within a single cisterna, which gradually moves from the cis to the trans face without the protein ever leaving it. Alternatively, the cisternae may remain fixed in position, while individual proteins are carried between them by specialized transport vesicles called COPI vesicles. Now, Pellett et al. have used modern molecular biology techniques to revisit this question, more than 25 years after members of the same group first obtained evidence suggesting the involvement of COPI vesicles. To do this, they labelled the proteins that reside within the Golgi of one cell green, and those within the Golgi of another cell, red. They then fused the two cells together, and traced the movement of labelled proteins between the two organelles. Proteins that are known to undergo anterograde transport were also transported between the two Golgi, whereas large protein aggregates were not. Super-resolution microscopy revealed that the transported proteins were carried in vesicles the size of COPI vesicles and surrounded by a coat protein that resembles COPI. Moreover, transport involved the adaptor protein ARF, which helps to load cargo into COPI vesicles. By providing evidence that Golgi resident proteins and proteins that normally undergo anterograde transport can be carried by COPI vesicles between two physically separate Golgi, Pellett et al. increase the weight of evidence that COPI vesicles may also be responsible for both retrograde and anterograde transport within the Golgi itself.
Type of Medium:
Online Resource
ISSN:
2050-084X
DOI:
10.7554/eLife.01296.001
DOI:
10.7554/eLife.01296.002
DOI:
10.7554/eLife.01296.003
DOI:
10.7554/eLife.01296.004
DOI:
10.7554/eLife.01296.005
DOI:
10.7554/eLife.01296.006
DOI:
10.7554/eLife.01296.007
DOI:
10.7554/eLife.01296.008
DOI:
10.7554/eLife.01296.009
DOI:
10.7554/eLife.01296.010
DOI:
10.7554/eLife.01296.011
DOI:
10.7554/eLife.01296.012
DOI:
10.7554/eLife.01296.013
DOI:
10.7554/eLife.01296.014
DOI:
10.7554/eLife.01296.015
DOI:
10.7554/eLife.01296.016
DOI:
10.7554/eLife.01296.017
DOI:
10.7554/eLife.01296.018
Language:
English
Publisher:
eLife Sciences Publications, Ltd
Publication Date:
2013
detail.hit.zdb_id:
2687154-3
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