In:
PLOS Genetics, Public Library of Science (PLoS), Vol. 19, No. 2 ( 2023-2-3), p. e1010618-
Abstract:
Transcription termination factor Rho is known for its ubiquitous role in suppression of pervasive, mostly antisense, transcription. In the model Gram-positive bacterium Bacillus subtilis , de-repression of pervasive transcription by inactivation of rho revealed the role of Rho in the regulation of post-exponential differentiation programs. To identify other aspects of the regulatory role of Rho during adaptation to starvation, we have constructed a B . subtilis strain (Rho + ) that expresses rho at a relatively stable high level in order to compensate for its decrease in the wild-type cells entering stationary phase. The RNAseq analysis of Rho + , WT and Δ rho strains (expression profiles can be visualized at http://genoscapist.migale.inrae.fr/seb_rho/ ) shows that Rho over-production enhances the termination efficiency of Rho-sensitive terminators, thus reducing transcriptional read-through and antisense transcription genome-wide. Moreover, the Rho + strain exhibits global alterations of sense transcription with the most significant changes observed for the AbrB, CodY, and stringent response regulons, forming the pathways governing the transition to stationary phase. Subsequent physiological analyses demonstrated that maintaining rho expression at a stable elevated level modifies stationary phase-specific physiology of B . subtilis cells, weakens stringent response, and thereby negatively affects the cellular adaptation to nutrient limitations and other stresses, and blocks the development of genetic competence and sporulation. These results highlight the Rho-specific termination of transcription as a novel element controlling stationary phase. The release of this control by decreasing Rho levels during the transition to stationary phase appears crucial for the functionality of complex gene networks ensuring B . subtilis survival in stationary phase.
Type of Medium:
Online Resource
ISSN:
1553-7404
DOI:
10.1371/journal.pgen.1010618
DOI:
10.1371/journal.pgen.1010618.g001
DOI:
10.1371/journal.pgen.1010618.g002
DOI:
10.1371/journal.pgen.1010618.g003
DOI:
10.1371/journal.pgen.1010618.g004
DOI:
10.1371/journal.pgen.1010618.g005
DOI:
10.1371/journal.pgen.1010618.g006
DOI:
10.1371/journal.pgen.1010618.g007
DOI:
10.1371/journal.pgen.1010618.g008
DOI:
10.1371/journal.pgen.1010618.g009
DOI:
10.1371/journal.pgen.1010618.g010
DOI:
10.1371/journal.pgen.1010618.s001
DOI:
10.1371/journal.pgen.1010618.s002
DOI:
10.1371/journal.pgen.1010618.s003
DOI:
10.1371/journal.pgen.1010618.s004
DOI:
10.1371/journal.pgen.1010618.s005
DOI:
10.1371/journal.pgen.1010618.s006
DOI:
10.1371/journal.pgen.1010618.s007
DOI:
10.1371/journal.pgen.1010618.s008
DOI:
10.1371/journal.pgen.1010618.s009
DOI:
10.1371/journal.pgen.1010618.s010
DOI:
10.1371/journal.pgen.1010618.s011
DOI:
10.1371/journal.pgen.1010618.s012
DOI:
10.1371/journal.pgen.1010618.s013
DOI:
10.1371/journal.pgen.1010618.s014
DOI:
10.1371/journal.pgen.1010618.s015
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2023
detail.hit.zdb_id:
2186725-2
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