In:
International Archives of Allergy and Immunology, S. Karger AG, Vol. 159, No. 3 ( 2012), p. 235-242
Abstract:
〈 b 〉 〈 i 〉 Background: 〈 /i 〉 〈 /b 〉 Over the past years, dust of green coffee beans has become known to be a relevant cause for occupational type I allergies. Up to now, allergy diagnostics is based on native green coffee bean extract which exhibits insufficient specificity due to interfering substances as well as batch-to-batch variations. No coffee allergen has been described on the molecular level so far. The aim of this study was to identify the first allergen of green coffee. 〈 b 〉 〈 i 〉 Methods: 〈 /i 〉 〈 /b 〉 The allergenicity of native green coffee bean extracts was analyzed by means of ImmunoCAP in sera of 17 symptomatic coffee workers. A 〈 i 〉 Coffea arabica 〈 /i 〉 pJuFo cDNA phage display library was constructed and screened for IgE binding to coffee proteins with 2 sera from allergic coffee workers. By sequence analysis, a new coffee allergen (Cof a 1) was identified, expressed in 〈 i 〉 Escherichia coli, 〈 /i 〉 and evaluated by Western blots. The frequency of sensitization was investigated by ELISA screening. 〈 b 〉 〈 i 〉 Results: 〈 /i 〉 〈 /b 〉 The Cof a 1 cDNA encoded a 32-kDa 〈 i 〉 C. arabica 〈 /i 〉 class III chitinase. Serum IgE antibodies to the recombinant allergen were found in 3 out of 17 symptomatic coffee workers (18%), whereas only 2 of them reacted to the commercial allergy test. 〈 b 〉 〈 i 〉 Conclusions: 〈 /i 〉 〈 /b 〉 A class III chitinase of 〈 i 〉 C. arabica 〈 /i 〉 was identified to be the first known coffee allergen Cof a 1. It may have a relevant potential for the specific diagnosis of coffee sensitization.
Type of Medium:
Online Resource
ISSN:
1018-2438
,
1423-0097
Language:
English
Publisher:
S. Karger AG
Publication Date:
2012
detail.hit.zdb_id:
1482722-0
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