In:
Journal of the American Society of Nephrology, Ovid Technologies (Wolters Kluwer Health), Vol. 32, No. 3 ( 2021-3), p. 553-562
Abstract:
Regulation of endothelial cells is important in many biologic processes, including development, organ function, and disease. The kidney vasculature is highly sensitive to hypoxic injury and has a limited capacity for repair. AKI as a result of decreased blood flow is common, and there are no current therapies. MicroRNAs are small noncoding RNAs that inhibit expression of target genes. Endothelial-derived miR-17∼92 is a cluster of microRNAs critical for endothelial function and repair during AKI in mice. Furthermore, pharmacologic treatment with mimics of the cluster mitigates AKI, promoting angiogenesis. These microRNAs are the first potential therapeutic target for kidney endothelial damage after AKI, and mimics may be broadly applicable to disease processes that involve endothelial injury. Background Damage to the renal microvasculature is a hallmark of renal ischemia-reperfusion injury (IRI)–mediated AKI. The miR-17∼92 miRNA cluster (encoding miR-17 , -18a , -19a , -20a , -19b-1 , and -92a-1 ) regulates angiogenesis in multiple settings, but no definitive role in renal endothelium during AKI pathogenesis has been established. Methods Antibodies bound to magnetic beads were utilized to selectively enrich for renal endothelial cells from mice. Endothelial-specific miR-17∼92 knockout ( miR-17∼92 endo−/− ) mice were generated and given renal IRI. Mice were monitored for the development of AKI using serum chemistries and histology and for renal blood flow using magnetic resonance imaging (MRI) and laser Doppler imaging. Mice were treated with miRNA mimics during renal IRI, and therapeutic efficacies were evaluated. Results miR-17 , -18a , -20a , -19b , and pri–miR-17∼92 are dynamically regulated in renal endothelial cells after renal IRI. miR-17∼92 endo−/− exacerbates renal IRI in male and female mice. Specifically, miR-17∼92 endo−/− promotes renal tubular injury, reduces renal blood flow, promotes microvascular rarefaction, increases renal oxidative stress, and promotes macrophage infiltration to injured kidneys. The potent antiangiogenic factor thrombospondin 1 (TSP1) is highly expressed in renal endothelium in miR-17∼92 endo−/− after renal IRI and is a target of miR-18a and miR-19a/b . miR-17∼92 is critical in the angiogenic response after renal IRI, which treatment with miR-18a and miR-19b mimics can mitigate. Conclusions These data suggest that endothelial-derived miR-17∼92 stimulates a reparative response in damaged renal vasculature during renal IRI by regulating angiogenic pathways.
Type of Medium:
Online Resource
ISSN:
1046-6673
,
1533-3450
DOI:
10.1681/ASN.2020050717
Language:
English
Publisher:
Ovid Technologies (Wolters Kluwer Health)
Publication Date:
2021
detail.hit.zdb_id:
2029124-3
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