In:
PLOS ONE, Public Library of Science (PLoS), Vol. 16, No. 4 ( 2021-4-28), p. e0249899-
Abstract:
Rocky Mountain elk ( Cervus canadensis ) populations have significant economic implications to the cattle industry, as they are a major reservoir for Brucella abortus in the Greater Yellowstone area. Vaccination attempts against intracellular bacterial diseases in elk populations have not been successful due to a negligible adaptive cellular immune response. A lack of genomic resources has impeded attempts to better understand why vaccination does not induce protective immunity. To overcome this limitation, PacBio, Illumina, and Hi-C sequencing with a total of 686-fold coverage was used to assemble the elk genome into 35 pseudomolecules. A robust gene annotation was generated resulting in 18,013 gene models and 33,422 mRNAs. The accuracy of the assembly was assessed using synteny to the red deer and cattle genomes identifying several chromosomal rearrangements, fusions and fissions. Because this genome assembly and annotation provide a foundation for genome-enabled exploration of Cervus species, we demonstrate its utility by exploring the conservation of immune system-related genes. We conclude by comparing cattle immune system-related genes to the elk genome, revealing eight putative gene losses in elk.
Type of Medium:
Online Resource
ISSN:
1932-6203
DOI:
10.1371/journal.pone.0249899
DOI:
10.1371/journal.pone.0249899.g001
DOI:
10.1371/journal.pone.0249899.t001
DOI:
10.1371/journal.pone.0249899.t002
DOI:
10.1371/journal.pone.0249899.t003
DOI:
10.1371/journal.pone.0249899.t004
DOI:
10.1371/journal.pone.0249899.s001
DOI:
10.1371/journal.pone.0249899.s002
DOI:
10.1371/journal.pone.0249899.s003
DOI:
10.1371/journal.pone.0249899.s004
DOI:
10.1371/journal.pone.0249899.s005
DOI:
10.1371/journal.pone.0249899.s006
DOI:
10.1371/journal.pone.0249899.s007
DOI:
10.1371/journal.pone.0249899.s008
DOI:
10.1371/journal.pone.0249899.s009
DOI:
10.1371/journal.pone.0249899.s010
DOI:
10.1371/journal.pone.0249899.r001
DOI:
10.1371/journal.pone.0249899.r002
DOI:
10.1371/journal.pone.0249899.r003
DOI:
10.1371/journal.pone.0249899.r004
DOI:
10.1371/journal.pone.0249899.r005
DOI:
10.1371/journal.pone.0249899.r006
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2021
detail.hit.zdb_id:
2267670-3
Bookmarklink