In:
Frontiers in Immunology, Frontiers Media SA, Vol. 14 ( 2023-7-25)
Abstract:
CD4 + T cells play a central role in the adaptive immune response through their capacity to activate, support and control other immune cells. Although these cells have become the focus of intense research, a comprehensive understanding of the underlying regulatory networks that orchestrate CD4 + T cell function and activation is still incomplete. Here, we analyzed a large transcriptomic dataset consisting of 48 different human CD4 + T cell conditions. By performing reverse network engineering, we identified six common denominators of CD4 + T cell functionality (CREB1, E2F3, AHR, STAT1, NFAT5 and NFATC3). Moreover, we also analyzed condition-specific genes which led us to the identification of the transcription factor MEOX1 in T reg cells. Expression of MEOX1 was comparable to FOXP3 in T reg cells and can be upregulated by IL-2. Epigenetic analyses revealed a permissive epigenetic landscape for MEOX1 solely in T reg cells. Knockdown of MEOX1 in T reg cells revealed a profound impact on downstream gene expression programs and T reg cell suppressive capacity. These findings in the context of CD4 + T cells contribute to a better understanding of the transcriptional networks and biological mechanisms controlling CD4 + T cell functionality, which opens new avenues for future therapeutic strategies.
Type of Medium:
Online Resource
ISSN:
1664-3224
DOI:
10.3389/fimmu.2023.1107397
DOI:
10.3389/fimmu.2023.1107397.s001
DOI:
10.3389/fimmu.2023.1107397.s002
DOI:
10.3389/fimmu.2023.1107397.s003
DOI:
10.3389/fimmu.2023.1107397.s004
DOI:
10.3389/fimmu.2023.1107397.s005
DOI:
10.3389/fimmu.2023.1107397.s006
DOI:
10.3389/fimmu.2023.1107397.s007
DOI:
10.3389/fimmu.2023.1107397.s008
Language:
Unknown
Publisher:
Frontiers Media SA
Publication Date:
2023
detail.hit.zdb_id:
2606827-8
Bookmarklink
