In:
Journal of Bacteriology, American Society for Microbiology, Vol. 185, No. 24 ( 2003-12-15), p. 7092-7102
Abstract:
Pathogenicity
of the gram-negative plant pathogen Xanthomonas campestris pv.
vesicatoria depends on a type III secretion (TTS) system which translocates bacterial effector proteins into the plant cell. Previous
transcriptome analysis identified a genome-wide regulon of putative virulence genes that are coexpressed with the TTS system. In this
study, we characterized two of these genes, xopC and xopJ . Both genes encode Xanthomonas outer proteins
(Xops) that were shown to be secreted by the TTS system. In addition, type III-dependent translocation of both proteins into the plant cell
was demonstrated using the AvrBs3 effector domain as a reporter. XopJ belongs to the AvrRxv/YopJ family of effector proteins from plant and
animal pathogenic bacteria. By contrast, XopC does not share significant homology to proteins in the database. Sequence analysis
revealed that the xopC locus contains several features that
are reminiscent of pathogenicity islands. Interestingly, the xopC region is flanked by 62-bp inverted repeats that are also
associated with members of the Xanthomonas avrBs3 effector
family. Besides xopC , a second gene of the locus, designated hpaJ , was shown to be coexpressed with the TTS system. hpaJ encodes a protein with similarity to transglycosylases
and to the Pseudomonas syringae pv. maculicola protein
HopPmaG. HpaJ secretion and translocation by the X. campestris pv. vesicatoria TTS system was not detectable, which is consistent with
its predicted Sec signal and a putative function as transglycosylase in the bacterial
periplasm.
Type of Medium:
Online Resource
ISSN:
0021-9193
,
1098-5530
DOI:
10.1128/JB.185.24.7092-7102.2003
Language:
English
Publisher:
American Society for Microbiology
Publication Date:
2003
detail.hit.zdb_id:
1481988-0
SSG:
12
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