In:
Acta Crystallographica Section D Biological Crystallography, International Union of Crystallography (IUCr), Vol. 56, No. 1 ( 2000-01-01), p. 73-75
Abstract:
m-Calpain constitutes the prototype of the superfamily of neutral calcium-activated cysteine proteinases. It is a heterodimer consisting of an 80 and a 30 kDa subunit. Recombinant full-length human m-calpain has been crystallized using macro-seeding techniques and vapour-diffusion methods. Two different monoclinic crystal forms (space group P 2 1 ) were obtained from a solution containing polyethylene glycol ( M W = 10 000) as a pecipitating agent. Complete data sets have been collected to 2.3 and 3.0 Å resolution using cryo-cooling conditions and synchrotron radiation. The unit-cell parameters are a = 64.86, b = 133.97, c = 78.00 Å, β = 102.43° and a = 51.80, b = 171.36, c = 64.66 Å, β = 94.78°, respectively. The V m values indicate that there is one heterodimer in each asymmetric unit.
Type of Medium:
Online Resource
ISSN:
0907-4449
DOI:
10.1107/S0907444999013748
Language:
Unknown
Publisher:
International Union of Crystallography (IUCr)
Publication Date:
2000
detail.hit.zdb_id:
2968623-4
SSG:
12
SSG:
13
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