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  • 1
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    Identification of periodontopathogen microorganisms by PCR technique
    National Library of Serbia ; 2008
    In:  Srpski arhiv za celokupno lekarstvo Vol. 136, No. 9-10 ( 2008), p. 476-480
    Details
    In: Srpski arhiv za celokupno lekarstvo, National Library of Serbia, Vol. 136, No. 9-10 ( 2008), p. 476-480
    Kurzfassung: Uvod Epidemioloski podaci iz citavog sveta ukazuju na veliku rasprostranjenost gingivitisa i parodontopatije, oboljenja potpornog aparata zuba. U etiopatogenezi oboljenja parodoncijuma kljucnu ulogu igraju razliciti rodovi Gram-negativnih bakterija, ponajvise striktnih anaeroba. Cilj rada Cilj rada je bio da se ispita postojanje genoma glavnih parodontopatogenih mikroorganizama Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans, Eikenella corrodens, Treponema denticola, Tanerella forsythia i Prevotella intermedia u razlicitim uzorcima poreklom iz usne duplje pacijenata s klinicki dijagnostikovanom parodontopatijom. Metod rada Kao bioloski materijal u kojem je dokazivano postojanje DNK mikroorganizama korisceni su zubni plak, tkivo zapaljene gingive i pljuvacka. Za otkrivanje bakterijskog genoma primenjena je multipleks tehnika reakcije lancanog umnozavanja (engl. polymerase chain reaction PCR), odnosno simultana amplifikacija gena dve razlicite bakterije. Rezultati S manjom ili vecom ucestaloscu, u svim ispitanim uzorcima utvrdjeno je postojanje parodontopatogenih mikroorganizama. U zubnom plaku osoba obolelih od parodontopatije najcesci je bio genom vrste Treponema denticola. U tkivu parodoncijuma otkriveno je u najvecem procentu postojanje genoma vrsta Tannerella forsythia i Treponema denticola, sto je odlika hronicnog oblika parodontopatije, a u pljuvacki ispitanika dominirale su Treponema denticola i Eikinella corrodens. Najmanje ukupno postojanje bakterija je zapazeno u pljuvacki. Zakljucak Primenjeni metod PCR ima veliku osetljivost i specificnost. Brzo i precizno otkrivanje mikroorganizama je veoma vazno za pravovremeno dijagnostikovanje infekcije, a samim tim i za prevenciju i lecenje parodontopatija. U svakodnevnoj klinickoj praksi optimalan bioloski materijal za dokazivanje parodontopatogena kod osoba obolelih od parodontopatije je zubni plak, koji se smatra pouzdanim pokazateljem zastupljenosti pojedinih bakterija u obolelom parodoncijumu.
    Materialart: Online-Ressource
    ISSN: 0370-8179 , 2406-0895
    URL: Article
    DOI: 10.2298/SARH0810476M
    Sprache: Englisch
    Verlag: National Library of Serbia
    Publikationsdatum: 2008
    ZDB Id: 2577665-4
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  • 2
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    Detection rates of periodontal bacteria and herpesviruses in different forms of periodontal disease
    Wiley ; 2020
    In:  Microbiology and Immunology Vol. 64, No. 12 ( 2020-12), p. 815-824
    Details
    In: Microbiology and Immunology, Wiley, Vol. 64, No. 12 ( 2020-12), p. 815-824
    Kurzfassung: The aim was to investigate the detection rates of periodontal bacteria ( Porphyromonas gingivalis, Tannerella forsythia, Prevotella intermedia , and Aggregatibacter actinomycetemcomitans ) and herpesviruses (herpes simplex virus‐1 [HSV‐1], cytomegalovirus [CMV] , and Epstein‐Barr virus [EBV]) in different forms and severity of periodontal disease, and to compare them with those in periodontally healthy subjects. One hundred and twenty‐nine patients participated in the study: 39 diagnosed with periodontal abscess (PA), 33 with necrotizing ulcerative periodontitis (NUP), 27 with chronic periodontitis (CP), and 30 participants with healthy periodontal tissue represented a healthy control group. All patients with periodontal disease (PA, NUP, and CP) were also divided into two groups according to the severity of their disease: moderate and severe periodontitis. The subgingival samples were collected from the periodontitis active sites and the detection of microorganisms was performed by end‐point polymerase chain reaction analyses. The results revealed significantly higher detection rates of P. gingivalis, T. forsythia , and P. intermedia in all three groups of patients with periodontitis than in healthy participants. The highest detection rate of A. actinomycetemcomitans was noticed in CP, which was significantly higher than that in PA, NUP, and healthy control. The occurrence of EBV was significantly higher in NUP than in CP and healthy participants. CMV was detected significantly more frequently in PA and NUP than in CP and healthy participants. Comparisons among healthy participants and patients with moderate and severe periodontitis showed significantly higher detection rates of EBV and CMV in patients with severe forms of periodontitis than in healthy participants and those with moderate periodontitis.
    Materialart: Online-Ressource
    ISSN: 0385-5600 , 1348-0421
    URL: Issue
    URL: Article
    DOI: 10.1111/mim.v64.12
    DOI: 10.1111/1348-0421.12857
    Sprache: Englisch
    Verlag: Wiley
    Publikationsdatum: 2020
    ZDB Id: 2102145-4
    SSG: 12
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  • 3
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    Interaction Between Fibronectin Fragments and Immunoglobulin G in Gingival Crevicular Fluid of Patients with Periodontal Disease/ Kompleksi fibronektinskih fragmenata i imunoglobulina G u gingivalnoj tečnosti osoba obolelih od parodontopatije
    Walter de Gruyter GmbH ; 2015
    In:  Stomatoloski glasnik Srbije Vol. 62, No. 2 ( 2015-06-1), p. 55-64
    Details
    In: Stomatoloski glasnik Srbije, Walter de Gruyter GmbH, Vol. 62, No. 2 ( 2015-06-1), p. 55-64
    Kurzfassung: Introduction Fibronectin (FN) can interact with immunoglobulin G (IgG) molecules affecting the process of physiological elimination and causing abnormal deposition of immune complexes. The aim of the study was to analyze interaction between FN fragments and IgG molecules with different glycosylation profiles in gingival crevicular fluid (GCF) of patients with periodontal disease and healthy controls. Material and Methods The study included 30 patients with moderate and advanced periodontitis and 22 healthy subjects. IgG and FN content in GCF were measured as well as the presence of FN and galactose expression on IgG molecules. Results IgG content in GCF was five times higher in patients with moderate (p 〈 0.01) and eight time higher in patients with advanced periodontitis (p 〈 0.001) compared to healthy subjects. Also, hypogalactosylated forms of IgG were found in higher concentration in GCF of patients with advanced periodontitis compared to moderate periodontitis and healthy subjects (p 〈 0.05). FN fragments of molecular mass 48 - 53 kDa were the most commonly found fragments in all three groups. Furthermore, in patients with advanced periodontitis, fibronectin fragments were attached to IgG molecules. Conclusion IgG and FN fragments form complexes in GCF in patients with periodontal disease and healthy subjects
    Materialart: Online-Ressource
    ISSN: 1452-3701
    URL: Article
    DOI: 10.1515/sdj-2015-0006
    Sprache: Unbekannt
    Verlag: Walter de Gruyter GmbH
    Publikationsdatum: 2015
    ZDB Id: 2381437-8
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  • 4
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    The role of TERT-CLPTM1L SNPs, hTERT expression and telomere length in the pathogenesis of oral squamous cell carcinoma
    Nihon University School of Dentistry ; 2016
    In:  Journal of Oral Science Vol. 58, No. 4 ( 2016), p. 449-458
    Details
    In: Journal of Oral Science, Nihon University School of Dentistry, Vol. 58, No. 4 ( 2016), p. 449-458
    Materialart: Online-Ressource
    ISSN: 1343-4934 , 1880-4926
    URL: Article
    DOI: 10.2334/josnusd.16-0108
    Sprache: Englisch
    Verlag: Nihon University School of Dentistry
    Publikationsdatum: 2016
    ZDB Id: 2197202-3
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  • 5
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    Hypogalactosylation of immunoglobulin G (IgG) of gingival fluid and saliva at the patient with periodontal disease
    National Library of Serbia ; 2006
    In:  Serbian Dental Journal Vol. 53, No. 1 ( 2006), p. 7-16
    Details
    In: Serbian Dental Journal, National Library of Serbia, Vol. 53, No. 1 ( 2006), p. 7-16
    Kurzfassung: Promenjena glikozilacija imunoglobulina G (IgG), pre svega ekspresija terminalne galaktoze, utice na brojne funkcije ovih imunoglobulina i korelira sa stepenom zapaljenja u mnogim bolestima. Cilj rada: U ovom radu analizirana je distribucija IgG podklasa i sadrzaj terminalne galaktoze u njima, u pljuvacci i gingivalnoj tecnosti bolesnika sa parodontopatijom razlicitog stepena inflamacije gingive. Materijal i metod: Kao materijal u ispitivanjima koriscena je gingivalna tecnost i pljuvacka 30 odraslih osoba sa klinickom dijagnozom parodontopatija i 20 osoba sa zdravim parodoncijumom. Kvantifikacija IgG uradjena je ?dot-blot? postupkom, a odredjivanje terminalne galaktoze lektinskim imunoblot postupkom. Rezultati: Raspodela IgG podklasa u obe tecnosti se razlikovala kod parodontopatija i u kontrolnim uzorcima. I u pljuvacci i u gingivalnoj tecnosti obolelih, kvantitativno je dominirala IgG2 podklasa, nezavisno od parodontalnog statusa. U IgG obe oralne tecnosti terminalna galaktoza je bila eksprimirana kod osoba sa zdravim parodoncijumom (kontrola) i kod osoba sa pocetnom (inicijalnom) parodontopatijom, dok kod osoba sa uznapredovalom parodontopatijom ekspresija ovog secera nije registrovana ni u jednoj od ove dve tecnosti. Zakljucak: Rezultati ovih istrazivanja ukazuju da postoji pomeranje prema hipogalaktozilovanim IgG glikoformama tokom procesa inflamacije gingive u obolelih od parodontopatije.
    Materialart: Online-Ressource
    ISSN: 0039-1743 , 1452-3701
    URL: Article
    DOI: 10.2298/SGS0601007S
    Sprache: Englisch
    Verlag: National Library of Serbia
    Publikationsdatum: 2006
    ZDB Id: 2381437-8
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  • 6
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    Association of Fibronectin With Hypogalactosylated Immunoglobulin G in Gingival Crevicular Fluid in Periodontitis
    Wiley ; 2010
    In:  Journal of Periodontology Vol. 81, No. 10 ( 2010-10), p. 1472-1480
    Details
    In: Journal of Periodontology, Wiley, Vol. 81, No. 10 ( 2010-10), p. 1472-1480
    Kurzfassung: Background: Fibronectin (FN) can bind to immunoglobulins (Ig), influencing both the normal clearance and abnormal deposition of circulating immune complexes. This study focuses on the possible interaction between FN and IgG present in gingival crevicular fluid (GCF) of periodontitis patients and periodontally healthy controls, with the aim to determine whether such interaction may be connected with the glycosylation profile of IgG and, thus, consequentional in accumulation of proinflammatory IgG in periodontal pockets. Methods: The study included 30 patients with initial or advanced periodontitis, and 13 periodontally healthy subjects. GCF IgG was purified and analyzed for the presence of FN and its fragments and for galactose expression. Results: IgG isolated from GCF contained FN, which was bound to the IgG heavy chains. IgG from GCF of advanced periodontitis patients contained high levels of hypogalactosylated IgG, and bound more FN than IgG from GCF of the other two groups, which contained low levels of this glycoform. FN was in a degraded form in GCF from all studied patients, and a fragment of 48‐ to 53‐kDa molecular mass seemed to be the sole one linked to IgG. Conclusions: IgG and the FN fragment of 48 to 53 kDa in GCF of periodontitis patients and periodontally healthy subjects are physically connected. This fragment was linked to the heavy chains of IgG and the reaction seemed to be particularly intensive with IgG from advanced periodontitis, which contained significantly less galactose in its sugar chains.
    Materialart: Online-Ressource
    ISSN: 0022-3492 , 1943-3670
    URL: Article
    DOI: 10.1902/jop.2010.100053
    Sprache: Englisch
    Verlag: Wiley
    Publikationsdatum: 2010
    ZDB Id: 2040047-0
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  • 7
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    Hypogalactosylation of Salivary and Gingival Fluid Immunoglobulin G in Patients With Advanced Periodontitis
    Wiley ; 2006
    In:  Journal of Periodontology Vol. 77, No. 11 ( 2006-11), p. 1887-1893
    Details
    In: Journal of Periodontology, Wiley, Vol. 77, No. 11 ( 2006-11), p. 1887-1893
    Kurzfassung: Background: Altered glycosylation of immunoglobulin G (IgG) has been found to affect certain immunological activities of IgG and to correlate with increased inflammation in various disease states. This work deals with the changes in distribution and galactosylation of IgG subclasses present in saliva and gingival crevicular fluid (GCF) of patients with initial and advanced periodontitis and of normal controls. Methods: IgG subclasses were quantified by dot‐blot assay, and the degrees of expression of galactose in the total IgG and its individual subclasses were estimated by lectin immunoblot assay after sodium dodecyl sulfate‐polyacrylamide gel electrophoresis (SDS‐PAGE) separation of IgG and by capture enzyme‐linked immunosorbent assay (ELISA), using biotinylated Ricinus communis (RCA‐I) and Bandeiraea simplicifolia (BS‐II) lectins. Results: The distribution of IgG subclasses in both fluids was found to differ in periodontal patients compared to normal controls. In the periodontitis saliva and GCF, the IgG2 subclass dominated quantitatively, regardless of periodontal status. However, galactose was found to be expressed in IgG heavy chains in normal controls and patients with initial periodontitis but not, or at barely detectable levels, in advanced periodontitis. Conclusion: The results suggest that the shift toward hypogalactosylated glycoforms may occur during the process of inflammation of the gingiva.
    Materialart: Online-Ressource
    ISSN: 0022-3492 , 1943-3670
    URL: Article
    DOI: 10.1902/jop.2006.060049
    Sprache: Englisch
    Verlag: Wiley
    Publikationsdatum: 2006
    ZDB Id: 2040047-0
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  • 8
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    The effect of exercise during sport training on levels of salivary diagnostic markers
    National Library of Serbia ; 2021
    In:  Srpski arhiv za celokupno lekarstvo Vol. 149, No. 11-12 ( 2021), p. 660-665
    Details
    In: Srpski arhiv za celokupno lekarstvo, National Library of Serbia, Vol. 149, No. 11-12 ( 2021), p. 660-665
    Kurzfassung: Introduction/Objective. The aim of this study was to determine the changes in concentrations of urea, creatinine, uric acid, proteins, aspartate aminotransferase (AST), creatine kinase (CK), and salivary amylase in saliva samples collected before, immediately after, and 30 minutes after physical activity performed during basketball and mixed martial arts (MMA) training. Methods. Twenty-two athletes, 11 basketball players and 11 MMA fighters, 18 men and four women, aged 15?24 years, participated in the study. Saliva samples were collected using sterile saliva containers (Salivette?) from all participants before training (sample 1), immediately after (sample 2), and 30 minutes after training (sample 3). The levels of all investigated biomarkers were measured spectrophotometrically using a biochemical analyzer. Results. Statistically significant differences were present among samples 1, 2, and, 3 in the concentrations of urea, AST, and CK in samples collected from MMA fighters (Friedman test). Among three samples taken from basketball players, the significant differences were not observed for the analyzed parameters. When concentrations of all diagnostic markers were compared between basketball and MMA independently for samples 1, 2, and 3, statistically significant differences (Mann?Whitney U-test) existed in concentrations of urea, uric acid, proteins, and AST. Conclusion. Based on the results of the present study, the influence of the exercise on the levels of salivary diagnostic markers, such as urea, AST, and CK, is more evident during MMA than basketball training. Saliva composition of MMA fighters and basketball players differ in terms of levels of urea, uric acid, proteins, and AST.
    Materialart: Online-Ressource
    ISSN: 0370-8179 , 2406-0895
    URL: Article
    DOI: 10.2298/SARH210913095B
    Sprache: Englisch
    Verlag: National Library of Serbia
    Publikationsdatum: 2021
    ZDB Id: 2577665-4
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  • 9
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    Estimation of total bacteria by real-time PCR in patients with periodontal disease
    National Library of Serbia ; 2016
    In:  Srpski arhiv za celokupno lekarstvo Vol. 144, No. 1-2 ( 2016), p. 10-14
    Details
    In: Srpski arhiv za celokupno lekarstvo, National Library of Serbia, Vol. 144, No. 1-2 ( 2016), p. 10-14
    Kurzfassung: Introduction. Periodontal diseases are associated with the presence of elevated levels of bacteria within the gingival crevice. Objective. The aim of this study was to evaluate a total amount of bacteria in subgingival plaque samples in patients with a periodontal disease. Methods. A quantitative evaluation of total bacteria amount using quantitative real-time polymerase chain reaction (qRT-PCR) was performed on 20 samples of patients with ulceronecrotic periodontitis and on 10 samples of healthy subjects. The estimation of total bacterial amount was based on gene copy number for 16S rRNA that was determined by comparing to Ct values / gene copy number of the standard curve. Results. A statistically significant difference between average gene copy number of total bacteria in periodontal patients (2.55.107) and healthy control (2.37.106) was found (p=0.01). Also, a trend of higher numbers of the gene copy in deeper periodontal lesions (〉7 mm) was confirmed by a positive value of coefficient of correlation (r=0.073). Conclusion. The quantitative estimation of total bacteria based on gene copy number could be an important additional tool in diagnosing periodontitis.
    Materialart: Online-Ressource
    ISSN: 0370-8179 , 2406-0895
    URL: Article
    DOI: 10.2298/SARH1602010B
    Sprache: Englisch
    Verlag: National Library of Serbia
    Publikationsdatum: 2016
    ZDB Id: 2577665-4
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  • 10
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    Use of saliva as a diagnostic fluid in dentistry
    National Library of Serbia ; 2005
    In:  Srpski arhiv za celokupno lekarstvo Vol. 133, No. 7-8 ( 2005), p. 372-378
    Details
    In: Srpski arhiv za celokupno lekarstvo, National Library of Serbia, Vol. 133, No. 7-8 ( 2005), p. 372-378
    Kurzfassung: Pljuvacka je sekret pljuvacnih i mukoznih zlezda koji je znacajan za odrzavanje oralnog zdravlja. Poslednjih decenija sve vise se razmatra mogucnost njene primene kao zamena krvi, odnosno krvnom serumu i plazme, radi dokazivanja pokazatelja sistemskih oboljenja, kao i za nadgledanje primene lekova, droga i hormona. Analiziranje biohemijskog sastava pljuvacke posebno je znacajno u stomatologiji za procenu rizika nastanka, pracenja toka bolesti i kontrole rezultata primenjenog lecenja zubnog karijesa, parodontopatije, oralnih karcinoma i infektivnih oboljenja usne duplje. U proceni rizika nastanka karijesa na zubima rutinski se izvode ispitivanja kojima se odredjuju kolicina izlucene pljuvacke, njen puferski kapacitet i stepen kolonizacije usne duplje kariogenim vrstama bakterija. Jednostavnost izvodjenja ovih ispitivanja omogucava da ih u stomatoloskoj ordinaciji primenjuje lekar prakticar, sto predstavlja veliku pomoc u proceni rizika za nastanak zubnog karijesa. Zbog bliskog kontakta s oralnim tkivima, pljuvacka sadrzi brojne biohemijske pokazatelje patoloskih procesa lokalizovanih u ovim tkivima (enzimi, imunoglobulini, ostali proteini, fenotipski pokazatelji). Ispitivanje pomenutih pokazatelja je znacajno kao dopuna klinickom dijagnostikovanju ukoliko ono ne pruza dovoljno informacija.
    Materialart: Online-Ressource
    ISSN: 0370-8179 , 2406-0895
    URL: Article
    DOI: 10.2298/SARH0508372T
    Sprache: Englisch
    Verlag: National Library of Serbia
    Publikationsdatum: 2005
    ZDB Id: 2577665-4
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