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  • 1
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    Online Resource
    Analysis of TGFB1, CD105 and FSP1 expression in human granulosa cells during a 7-day primary in vitro culture
    Walter de Gruyter GmbH ; 2020
    In:  Medical Journal of Cell Biology Vol. 8, No. 4 ( 2020-12-31), p. 152-157
    Details
    In: Medical Journal of Cell Biology, Walter de Gruyter GmbH, Vol. 8, No. 4 ( 2020-12-31), p. 152-157
    Abstract: The human granulosa cells (GCs) surround the oocyte and form the ovarian follicle’s proper architecture. These sub-populations include mural granulosa cells, antral granulosa cells, and cumulus granulosa cells. Their main functions are to support the oocyte’s growth (cumulus granulosa cells) and estradiol production (mural granulosa cells). After ovulation, the granulosa cells transform into the luteal cells of the corpus luteum and produce progesterone. Our study investigated the expression profile of three genes: TGFB1, CD105, and FSP1 during a 7-day in vitro culture. The analysis was conducted using the RT-qPCR technique. Changes in the expression of CD105 and FSP1 could be observed during the 7-day in vitro culture. In the case of TGFB, the expression remained at a similar level, with no statistically significant differences observed. Running title : Expression of TGFB1, CD105 and FSP1 in granulosa cells
    Type of Medium: Online Resource
    ISSN: 2544-3577
    URL: Article
    DOI: 10.2478/acb-2020-0019
    Language: English
    Publisher: Walter de Gruyter GmbH
    Publication Date: 2020
    detail.hit.zdb_id: 2977877-3
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  • 2
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    Exosomes and their possible applications in the management of gestational diabetes
    Walter de Gruyter GmbH ; 2020
    In:  Medical Journal of Cell Biology Vol. 8, No. 4 ( 2020-12-31), p. 146-151
    Details
    In: Medical Journal of Cell Biology, Walter de Gruyter GmbH, Vol. 8, No. 4 ( 2020-12-31), p. 146-151
    Abstract: Gestational diabetes mellitus (GDM) is thought to be the most common metabolic gestational complication. Its prevalence has been continuously increasing in recent decades along with the rising epidemic of obesity in modern societies. GDM is associated with an increased risk of fetal growth abnormalities, birth traumas, and several neonatal complications. Widely available screening tools, fasting glucose measurements, combined with oral glucose tolerance test results, contribute to the reduction of the risk of those complications. Nevertheless, we are still looking for novel reliable early markers of GDM. It has been established that high 1 st -trimester exosome concentrations could predispose the development of GDM in later pregnancy. Exosomes can be easily isolated from various tissues and body fluids in pregnant patients. Due to this, extracellular vesicle concentration assessment appears as a new promising tool in the prediction of GDM at the preclinical stage of the disease. Furthermore, it has been found that women already diagnosed with GDM have significantly higher exosome concentrations compared with healthy individuals. These findings could help to elucidate the molecular pathogenesis of GDM. Exosomes are loaded with various molecules especially proteins, lipids, mRNAs, and microRNAs. Altered expression of numerous microRNAs and enzymes such as dipeptidyl peptidase-IV in exosomes isolated from patients with GDM may suggest their direct contribution to the mechanisms of glucose intolerance. This knowledge could be used in the development of new therapeutic strategies in patients with GDM. Nevertheless, it should be emphasized that these are only preliminary results that require further investigations. Running title : Exosomes in gestational diabetes
    Type of Medium: Online Resource
    ISSN: 2544-3577
    URL: Article
    DOI: 10.2478/acb-2020-0018
    Language: English
    Publisher: Walter de Gruyter GmbH
    Publication Date: 2020
    detail.hit.zdb_id: 2977877-3
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  • 3
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    Confirmation of differentiation clusters’ and endoglin markers preset in porcine buccal mucosa cells
    Walter de Gruyter GmbH ; 2020
    In:  Medical Journal of Cell Biology Vol. 8, No. 3 ( 2020-12-30), p. 118-123
    Details
    In: Medical Journal of Cell Biology, Walter de Gruyter GmbH, Vol. 8, No. 3 ( 2020-12-30), p. 118-123
    Abstract: Several genes, namely CD44, CD90, CD105 and PCNA may be important in differentiation of porcine mucosa cell cultures. These genes are, inter alia, responsible for cell adhesion to extracellular matrix and its constituent secretion, cytoskeleton organization, epithelial to mesenchymal transition or proper course of DNA replication. A total of 20 pubertal crossbred Landrace gilts bred on commercial farms were used to produce buccal mucosa cultures, which were harvested on the 7th, 15th and 30th day after initiation of the culture. Expression levels of CD44, CD90, CD105 and PCNA were evaluated employing Real-Time Quantitative Polymerase Chain Reaction. CD44, CD90 and PCNA showed an unchanged expression pattern. Expression of CD44 on day 7 was the highest of all factors measured. The greatest difference between the measurement on 7th and 30th day was found in the PCNA gene. These results broaden the understanding of the transcriptome changes in porcine buccal mucosa cells for the duration of in vitro cultivation. Nevertheless, it is very important to consider that the in vitro conditions do not fully reflect the changes taking place in the living organism. It appears that tissues of the oral cavity possess high regenerative potential, and constitute suitable model for wound healing investigation. Running title : Confirmation of differentiation clusters’ and endoglin markers preset in porcine buccal mucosa cells
    Type of Medium: Online Resource
    ISSN: 2544-3577
    URL: Article
    DOI: 10.2478/acb-2020-0015
    Language: English
    Publisher: Walter de Gruyter GmbH
    Publication Date: 2020
    detail.hit.zdb_id: 2977877-3
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  • 4
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    Histone demethylases JHDM1D, PHF2 and PHF8 expression pattern in granulosa cells obtained from patients undergoing IVF procedure during short-term IVC
    Walter de Gruyter GmbH ; 2021
    In:  Medical Journal of Cell Biology Vol. 9, No. 1 ( 2021-03-01), p. 1-7
    Details
    In: Medical Journal of Cell Biology, Walter de Gruyter GmbH, Vol. 9, No. 1 ( 2021-03-01), p. 1-7
    Abstract: Granulosa cells play an important role in follicle development, maturation, and atresia. They are a cellular source of the two most important ovarian steroids, namely, estradiol and progesterone and are also crucial for bidirectional communication with the oocyte, thus being involved in the regulation of its growth, development and function. Growing body of evidence suggests that granulosa cells cultured in vitro display stemness and transdifferentiation potential. Together with the fact that they can be easily collected during IVF procedures, these properties of GCs may be of particular interest for both regenerative medicine and transplantology. Establishment of in vitro cell culture and its thorough characterization, including molecular, is crucial for future potential utilization of human granulosa cells in design of engineered tissue grafts or cell-based therapies, in particular targeted at female infertility. Nevertheless, the transcriptomic alterations which may occur during in vitro culture of granulosa cells are still largely uncharacterized. The aim of this study was to examine expression changes of three genes encoding histone demethylases which serve as transcription coactivators in short term in vitro cell culture of human granulosa cells. The study groups consisted of 14 patients, aged 18–40 years undergoing in vitro fertilization (IVF). Expression level assessment was performed after 24 h, 48 h, 72 h, 96 h, 120 h, 144 h and 168 h of in vitro primary cell culture utilizing RT-qPCR technique. Upregulation of PHF2 expression in all time points of the culture was observed, whereas the tendency of JHDM1D and PHF8 was mainly to decrease in expression level. Further study on a larger population would be required in order to confirm the presented tendencies. Running title: Expression pattern of selected histone demethylases in human granulosa cells
    Type of Medium: Online Resource
    ISSN: 2544-3577
    URL: Article
    DOI: 10.2478/acb-2021-0001
    Language: English
    Publisher: Walter de Gruyter GmbH
    Publication Date: 2021
    detail.hit.zdb_id: 2977877-3
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  • 5
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    Mesenchymal stem cells – a historical overview
    Walter de Gruyter GmbH ; 2020
    In:  Medical Journal of Cell Biology Vol. 8, No. 2 ( 2020-09-05), p. 83-87
    Details
    In: Medical Journal of Cell Biology, Walter de Gruyter GmbH, Vol. 8, No. 2 ( 2020-09-05), p. 83-87
    Abstract: Mesenchymal stem cells are currently one of the most extensively studied topics in experimental medicine, given their unique properties in terms of immunomodulation, multiple factors secretion and homing to injured tissue sites. Such characteristics were proven to be invaluable in various disease management treatments, for example in cancer, tissue regeneration or immunologic/inflammatory-related disorders. MSCs were first isolated from bone marrow in 1960-1970’s and were characterized as cells with fibroblastoid shape and osteogenic potential, which form clonogenic colonies (CFU-F – colony-forming unit-fibroblast). Nowadays the term ‘mesenchymal stem cells’ is used in regards to all of the cells meeting minimal criteria published in 2006 by the International Society for Cellular Therapy, however the name ‘mesenchymal stromal cells’ has been suggested to be more appropriate. Regardless of the name controversy, these cells exhibit multilineage differentiation potential, self-renewal ability, adhere to plastic and express specific surface antigens. In 2011 the first commercial product based on MSCs was developed and many more are expected to emerge. This review focuses on a historical perspective concerning studies on MSCs, controversies regarding their name and their characteristics and clinical utilization. Running title : The history of mesenchymal stem cells
    Type of Medium: Online Resource
    ISSN: 2544-3577
    URL: Article
    DOI: 10.2478/acb-2020-0010
    Language: English
    Publisher: Walter de Gruyter GmbH
    Publication Date: 2020
    detail.hit.zdb_id: 2977877-3
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  • 6
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    The influence of osteogenic differentiation on the stem-like properties of adipose derived stem cells – an RT-qPCR study
    Walter de Gruyter GmbH ; 2020
    In:  Medical Journal of Cell Biology Vol. 8, No. 4 ( 2020-12-31), p. 158-163
    Details
    In: Medical Journal of Cell Biology, Walter de Gruyter GmbH, Vol. 8, No. 4 ( 2020-12-31), p. 158-163
    Abstract: ADSCs are readily accessible and widely available. Isolated through a minimally invasive procedure from adipose depots, they can be found at diverse body location, where they served various functions, including energy homeostasis. They can be obtained upon surgeries from otherwise waste tissues, like after excision of fat tissue or liposuction. In addition, due to the possibility to isolate many ADSCs, in vitro proliferation can be performed in a short time period, resulting in cells showing more predictable results[1]. For this study ADSCs were obtained from waste material following routing sterilization procedures of dogs. This study aimed to analyse the expression of MSC specific markers before and after in vitro differentiation of ASCs. Three positive and three negative markers were analysed, CD105, CD73, CD90, CD34 , CD14 and CD45 . There were significant differences detected in the expression of all of the genes, with most of them exhibiting notable downregulation. The only exception, CD14 showed major upregulation after the process of differentiation. These changes confirm the success of differentiation, as well as suggest that this process significantly lowers the stem-like ability of ASCs. This knowledge should serve as a reference for further molecular and clinical studies, possibly aiding the understanding of the internal mechanisms governing the differentiation and stemness of ASCs, to enable their widespread and safe application in regenerative medicine. Running title : Mesenchymal markers during ASC osteogenic differentiation
    Type of Medium: Online Resource
    ISSN: 2544-3577
    URL: Article
    DOI: 10.2478/acb-2020-0020
    Language: English
    Publisher: Walter de Gruyter GmbH
    Publication Date: 2020
    detail.hit.zdb_id: 2977877-3
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  • 7
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    Trophoblast stem cells - methods of isolation, histological and cellular characteristic, and their possible applications in human and animal models
    Walter de Gruyter GmbH ; 2020
    In:  Medical Journal of Cell Biology Vol. 8, No. 3 ( 2020-12-30), p. 95-100
    Details
    In: Medical Journal of Cell Biology, Walter de Gruyter GmbH, Vol. 8, No. 3 ( 2020-12-30), p. 95-100
    Abstract: The placenta is a part of feto-maternal unit that develops from the maternal decidua basalis and fetal-derived trophoblast cells. The regulation of its early development is extremely intricate, albeit the elusive trophoblast stem cells (TSCs) are thought to give rise to the fetal part of the placenta. TSCs may be isolated in both animal and human models. In detail, TSCs can be efficiently obtained from the early conceptus tissues – blastocysts or early placental tissue. The isolation of murine TSCs pave the way for analyses of human trophoblast cell lineages. Both human and animal stem cells retain similar characteristic properties – the ability for unrestricted self-renewal and differentiation into all trophoblast cell lines. Nevertheless, there are some essential differences across the various species which are especially pronounced when pertaining to their distinct optimal cell culture requirements. Moreover, there are several crucial discrepancies in the stemness marker gene transcription profiles between human and murine TSCs models. In vitro TSC models can be adapted to the elucidation of the pathophysiology of various reproductive complications. For instance, their properties may illustrate the conditions observed during the implantation or simulate the state of abnormal placentation. Observations gained from the experimental studies could potentially explain the cause of some cases of infertility, preeclampsia, and fetal growth abnormalities. Running title : Update on the trophoblast stem cells
    Type of Medium: Online Resource
    ISSN: 2544-3577
    URL: Article
    DOI: 10.2478/acb-2020-0012
    Language: English
    Publisher: Walter de Gruyter GmbH
    Publication Date: 2020
    detail.hit.zdb_id: 2977877-3
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  • 8
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    New Gene Markers Expressed in Porcine Oviductal Epithelial Cells Cultured Primary In Vitro Are Involved in Ontological Groups Representing Physiological Processes of Porcine Oocytes
    MDPI AG ; 2021
    In:  International Journal of Molecular Sciences Vol. 22, No. 4 ( 2021-02-19), p. 2082-
    Details
    In: International Journal of Molecular Sciences, MDPI AG, Vol. 22, No. 4 ( 2021-02-19), p. 2082-
    Abstract: Changes that occur within oviducts after fertilization are dependent on post-ovulation events, including oocyte-oviduct interactions. Although general processes are well-defined, the molecular basis are poorly understood. Recently, new marker genes involved in ‘cell development’, ‘cell growth’, ‘cell differentiation’ and ‘cell maturation’ processes have been identified in porcine oocytes. The aim of the study was to assess the expression profile of genes in primary in vitro cultured oviductal epithelial cells (OECs), clustered in Gene Ontology groups which enveloped markers also identified in porcine oocytes. OECs (from 45 gilts) were surgically removed and cultured in vitro for ≤ 30 days, and then subjected to molecular analyses. The transcriptomic and proteomic profiles of cells cultured during 7, 15 and 30 days were investigated. Additionally, morphological/histochemical analyzes were performed. The results of genes expression profiles were validated after using RT-qPCR. The results showed a significant upregulation of UNC45B, NOX4, VLDLR, ITGB3, FMOD, SGCE, COL1A2, LOX, LIPG, THY1 and downregulation of SERPINB2, CD274, TXNIP, CELA1, DDX60, CRABP2, SLC5A1, IDO1, ANPEP, FST. Detailed knowledge of the molecular pathways occurring in the OECs and the gametes that contact them may contribute both to developments of basic science of physiology, and new possibilities in advanced biotechnology of assisted reproduction.
    Type of Medium: Online Resource
    ISSN: 1422-0067
    URL: Article
    DOI: 10.3390/ijms22042082
    Language: English
    Publisher: MDPI AG
    Publication Date: 2021
    detail.hit.zdb_id: 2019364-6
    SSG: 12
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  • 9
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    COVID-19 Pandemic Is a Call to Search for Alternative Protein Sources as Food and Feed: A Review of Possibilities
    MDPI AG ; 2021
    In:  Nutrients Vol. 13, No. 1 ( 2021-01-05), p. 150-
    Details
    In: Nutrients, MDPI AG, Vol. 13, No. 1 ( 2021-01-05), p. 150-
    Abstract: The coronavirus disease 2019 (COVID-19) pandemic is a global health challenge with substantial adverse effects on the world economy. It is beyond any doubt that it is, again, a call-to-action to minimize the risk of future zoonoses caused by emerging human pathogens. The primary response to contain zoonotic diseases is to call for more strict regulations on wildlife trade and hunting. This is because the origins of coronaviruses such as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), SARS-CoV, Middle East respiratory syndrome coronavirus (MERS-CoV), as well as other viral pathogens (e.g., Ebola, HIV) are traceable to wild animals. Although COVID-19 is not related to livestock animals, the pandemic increased general attention given to zoonotic viral infections—the risk of which can also be associated with livestock. Therefore, this paper discusses the potential transformation of industrial livestock farming and the production of animal products, particularly meat, to decrease the risks for transmission of novel human pathogens. Plant-based diets have a number of advantages, but it is unrealistic to consider them as the only solution offered to the problem. Therefore, a search for alternative protein sources in insect-based foods and cultured meat, important technologies enabling safer meat production. Although both of these strategies offer a number of potential advantages, they are also subject to the number of challenges that are discussed in this paper. Importantly, insect-based foods and cultured meat can provide additional benefits in the context of ecological footprint, an aspect important in light of predicted climate changes. Furthermore, cultured meat can be regarded as ethically superior and supports better food security. There is a need to further support the implementation and expansion of all three approaches discussed in this paper, plant-based diets, insect-based foods, and cultured meat, to decrease the epidemiological risks and ensure a sustainable future. Furthermore, cultured meat also offers a number of additional benefits in the context of environmental impact, ethical issues, and food security.
    Type of Medium: Online Resource
    ISSN: 2072-6643
    URL: Article
    DOI: 10.3390/nu13010150
    Language: English
    Publisher: MDPI AG
    Publication Date: 2021
    detail.hit.zdb_id: 2518386-2
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  • 10
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    The advances in human oral biology and biotechnology
    Walter de Gruyter GmbH ; 2020
    In:  Medical Journal of Cell Biology Vol. 8, No. 2 ( 2020-09-05), p. 88-94
    Details
    In: Medical Journal of Cell Biology, Walter de Gruyter GmbH, Vol. 8, No. 2 ( 2020-09-05), p. 88-94
    Abstract: The oral cavity is a very specific and complicated structure. It plays the role of a speech apparatus but is also a gate and a preliminary processing plant for fuel nourishing the body. Analyzing the embryological development of the oral cavity, we can distinguish here the stages of growth, migration and fusion. This processes mainly involves the formation of branched arches that appear between the fourth and fifth weeks of fetal development. In recent years, interest in researching its various features has been still growing. After a thorough description and anatomical examination, researchers began to think about its other possibilities than just crushing, throwing, swallowing, bringing out the sounds and articulating words. It turns out that the whole range of its potential is not limited to just a few functions mentioned above. The following review article covers specific studies that have emerged most frequently over the past year. You will find here information about the microbiome of the oral cavity which plays an significant role in maintaining a proper physiological environment of the oral cavity, oral tumors and how the SCC or squamous cell carcinoma is histologically the most common and its treatment, even in the context of the Covid-19 pandemic, as well as the least discovered and still fascinating for scientists wound healing processes and unknown exosomes that can transport biological molecules in their original, intact and active form, including nucleic acids and proteins with their signaling pathways. Running title : The advances in human oral biology and biotechnology
    Type of Medium: Online Resource
    ISSN: 2544-3577
    URL: Article
    DOI: 10.2478/acb-2020-0011
    Language: English
    Publisher: Walter de Gruyter GmbH
    Publication Date: 2020
    detail.hit.zdb_id: 2977877-3
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