In:
Journal of Biochemical and Molecular Toxicology, Wiley, Vol. 32, No. 3 ( 2018-03)
Abstract:
The use of quail meat and eggs has made this animal important in recent years, with its low cost and high yields. Glutathione S‐transferases (GST, E.C.2.5.1.18) are an important enzyme family, which play a critical role in detoxification system. In our study, GST was purified from quail liver tissue with 47.88‐fold purification and 12.33% recovery by glutathione agarose affinity chromatography. The purity of enzyme was checked by SDS‐PAGE method and showed a single band. In addition, inhibition effects of (3a R ,4 S ,7 R ,7a S )‐2‐(4‐(( E )‐3‐(aryl)acryloyl)phenyl)‐3a,4,7,7a‐tetrahydro‐1 H ‐4,7methanoisoindole‐1,3(2 H )‐dion derivatives ( 1a–g ) were investigated on the enzyme activity. The inhibition parameters (IC 50 and K i values) were calculated for these compounds. IC 50 values of these derivatives ( 1a–e ) were found as 23.00, 15.75, 115.50, 10.00, and 28.75 μM, respectively. K i values of these derivatives ( 1a–e ) were calculated in the range of 3.04 ± 0.50 to 131.50 ± 32.50 μM. However, for f and g compounds, the inhibition effects on the enzyme were not found.
Type of Medium:
Online Resource
ISSN:
1095-6670
,
1099-0461
DOI:
10.1002/jbt.2018.32.issue-3
Language:
English
Publisher:
Wiley
Publication Date:
2018
detail.hit.zdb_id:
1481995-8
SSG:
12
Bookmarklink