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  • 1
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    Online Resource
    Genetic Diversity of Pectobacterium spp. on Potato in Serbia
    MDPI AG ; 2022
    In:  Microorganisms Vol. 10, No. 9 ( 2022-09-15), p. 1840-
    Details
    In: Microorganisms, MDPI AG, Vol. 10, No. 9 ( 2022-09-15), p. 1840-
    Abstract: Pectobacterium is a diverse genus which comprises of multiple destructive bacterial species which cause soft rot/blackleg/wilt disease complex in a wide variety of crops by employing high levels of virulence factors. During the 2018, 2019 and 2020 potato growing seasons, numerous outbreaks of bacterial wilt, stem blackleg and tuber soft rot were recorded, and symptomatic plant samples from ten localities in the Province of Vojvodina (Serbia) were collected and analysed. Bacterial soft-rot pathogens were detected in 63 samples using genus and species-specific primers. Through 16S rRNA Sanger sequencing of 19 representative isolates, the identity of P. brasiliense (73.7%), P. punjabense (15.8%), and P. carotovorum (10.5%) species were revealed. To further validate the identification, genotypic profiling of Pectobacterium strains using rep-PCR (ERIC, BOX, REP) was conducted for 25 selected isolates and the phylogenetic assessment based on four selected housekeeping genes (gyrA, recA, rpoA, and rpoS). Physiological and biochemical properties were analysed using basic microbiological tests and VITEK® 2 GN card, and pathogenicity was confirmed on cv. VR808 and cv. Desiree potato tubers and plants. This study confirmed the distinctiveness of the newly described P. punjabense in Serbia as well as the high diversity of Pectobacterium brasiliense and Pectobacterium carotovorum species in Serbia.
    Type of Medium: Online Resource
    ISSN: 2076-2607
    URL: Article
    DOI: 10.3390/microorganisms10091840
    Language: English
    Publisher: MDPI AG
    Publication Date: 2022
    detail.hit.zdb_id: 2720891-6
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  • 2
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    Online Resource
    The Pesticide Market in Serbia
    Walter de Gruyter GmbH ; 2022
    In:  Contemporary Agriculture Vol. 72, No. 1-2 ( 2022-06-01), p. 6-13
    Details
    In: Contemporary Agriculture, Walter de Gruyter GmbH, Vol. 72, No. 1-2 ( 2022-06-01), p. 6-13
    Abstract: The use of plant protection products (PPPs) has become a social concern regarding food safety and quality, and risks to human health and the environment. Accordingly, the agricultural microbials market is a steadily growing sector of the global agriculture industry. This paper evaluates the market of basic groups of pesticides in Serbia (namely herbicides, fungicides and insecticides), as well as other collectively presented plant protection products, with a particular emphasis on the biopesticides segment. The official data of the Serbian Plant Protection Directorate for the period 2000-2020 served as a primary source of data. The movement of PPPs and active ingredients (AIs) imports into Serbia were analyzed separately. The average values of PPP quantities imported into Serbia were computed for the period under consideration. The time series variability was evaluated using the coefficient of variation, whereas the dynamics of PPP import movements was evaluated using the rate of change. In the overall structure of the imported pesticides in Serbia, herbicides remain the dominant product type (57.0%), followed by fungicides (27.5%), insecticides (10.5%), and other PPPs (5.0%). The import of AIs for the production of herbicides claimed a share of 79.8%, whereas the share of AIs for the fungicide production approximated to 12.8% (followed by AIs for the insecticide production (6.7%) and AIs for the other groups of pesticides (0.7%). Biopesticides comprise a small share of the total crop protection market in Serbia. Bioinsecticides were found to be imported into Serbia in largest quantities (followed by biofungicides, bioacaricides, and biobactericides). No imports of bioherbicides have been recorded. According to the official data on the import of PPPs in Serbia in the period 2000–2020, it can be concluded that there is a positive growth trend, which is expected to continue in the future. Although the import of AIs shows a positive rate of change, no significant growth trends were recorded.
    Type of Medium: Online Resource
    ISSN: 2466-4774
    URL: Article
    DOI: 10.2478/contagri-2023-0002
    Language: English
    Publisher: Walter de Gruyter GmbH
    Publication Date: 2022
    detail.hit.zdb_id: 2676736-3
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  • 3
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    Online Resource
    Application of liquid chromatography with diode-array detector for determination of acetamiprid and 6-chloronicotinic acid residues in sweet cherry samples
    National Library of Serbia ; 2012
    In:  Pesticidi i fitomedicina Vol. 27, No. 4 ( 2012), p. 321-329
    Details
    In: Pesticidi i fitomedicina, National Library of Serbia, Vol. 27, No. 4 ( 2012), p. 321-329
    Abstract: A rapid and simple method for simultaneous determination of acetamiprid and its metabolite 6-chloronicotinic acid in sweet cherry samples has been developed. This residue analysis method is based on the reversed phase separation on C18 column with gradient elution. Analytes? determination and quantification were performed by high performance liquid chromatography (HPLC) with diode-array detector and chromatograms were extracted at 230 nm. Extraction efficiency experiments demonstrated the ability of this method to extract neonicotinoids from sweet cherry samples. These insecticides were extracted with a mixture of acetonitril/0.1N ammonium-chloride (8/2, v/v). The average recoveries of acetamiprid and 6-chlornicotinic acid from sweet cherry samples were in the range of 95-101% and 73-83%, respectively, with the associated relative standard deviations (RSDs) 〈5%. Expanded measurement uncertainties for the analyzed compounds were 2.7 and 3.01%. The limit of quantification (LOQ) was 10 ?g/kg and 30 ?g/kg for acetamiprid and 6-chloronicotinic acid, respectively. Thus, the developed HPLC/DAD method can be considered a useful tool for sensitive and rapid determination of acetamiprid and 6-chloronicotinic acid. Hence, the method may find further application in the analysis of real sweet cherry samples contaminated with these insecticides at a ppb level.
    Type of Medium: Online Resource
    ISSN: 1820-3949 , 2406-1026
    URL: Article
    DOI: 10.2298/PIF1204321L
    Language: English
    Publisher: National Library of Serbia
    Publication Date: 2012
    detail.hit.zdb_id: 2583550-6
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  • 4
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    Online Resource
    Antimicrobial efficiency of medicinal plants and their influence on cheeses quality
    Croatian Dairy Union ; 2020
    In:  Mljekarstvo Vol. 70, No. 1 ( 2020-01-08), p. 3-12
    Details
    In: Mljekarstvo, Croatian Dairy Union, Vol. 70, No. 1 ( 2020-01-08), p. 3-12
    Abstract: Mlijeko i mliječni proizvodi najstarija su i najčešće konzumirana hrana širom svijeta. Ističu se kao izvor visokokvalitetnih proteina i najvažniji izvor bioaktivnih peptida. Mliječni proteini imaju visoku nutritivnu vrijednost i izvanredna ljekovita svojstva. Poznati su kao potencijalni sastojci funkcionalne hrane koja promiče zdravlje, a mliječna industrija već je komercijalizirala mnoge mliječne proteine i proizvode na bazi peptida koji se mogu konzumirati kao dio redovite dnevne prehrane. Osim toga, sir je vrlo osjetljiv na kontaminaciju patogenim i mikroorganizmima kvarenja, što rezultira smanjenjem njegovog roka trajanja i uzrokuje ozbiljne rizike za zdravlje potrošača. Danas se prehrambena industrija sve više specijalizira, a metode obrade se stalno razvijaju kako bi zadovoljile potrebe i zahtjeve potrošača. Potrošači zahtijevaju proizvode koji su sigurni i po mogućnosti bez sintetskih aditiva, što je dovelo do potrebe za traženjem prirodnih alternativa. Ljekovito bilje i njihovi ekstrakti zanimljiva su prirodna alternativa za očuvanje i poboljšanje kvalitete sira. Neke su tvari pokazale dobre učinke protiv većine uzročnika kontaminacije sira, kao što su Listeria monocytogenes, Staphylococcus aureus i Salmonella spp. Ipak, nije sasvim jasno utječe li dodavanje ljekovitog bilja na promjene karakteristika sira. Cilj ovog rada bio je prikazati učinak dodanog ljekovitog bilja, začina i eteričnih ulja na sireve u funkciji zaštite i kvarenja uzrokovanog patogenima i mikroorganizmima, kao i njihovog utjecaja na kvalitetu sira.
    Type of Medium: Online Resource
    ISSN: 1846-4025 , 0026-704X
    URL: Journal
    URL: Article
    DOI: 10.15567/mljekarstvo
    DOI: 10.15567/mljekarstvo.2020.0102
    Language: Unknown
    Publisher: Croatian Dairy Union
    Publication Date: 2020
    detail.hit.zdb_id: 2268681-2
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  • 5
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    Online Resource
    Shared Odds of Borrelia and Rabies Virus Exposure in Serbia
    MDPI AG ; 2021
    In:  Pathogens Vol. 10, No. 4 ( 2021-03-28), p. 399-
    Details
    In: Pathogens, MDPI AG, Vol. 10, No. 4 ( 2021-03-28), p. 399-
    Abstract: Lyme borreliosis (LB) is the most common tick-borne disease in Serbia and other European countries. Rabies is a fatal zoonosis distributed worldwide and is caused by the rabies virus. Professionals at risk of rabies—including veterinarians, hunters, communal service workers, and forestry workers—overlap with some professions at a higher risk of exposure to tick bites and tick-borne pathogen infections. We hypothesized that individuals identified by the public health system as at risk of rabies virus infection, and consequently vaccinated against rabies virus, also share a higher likelihood of Borrelia exposure. To test our hypothesis, a case-control study was carried out during 2019 in Serbia to determine the seroprevalence of anti-Borrelia antibodies in two case groups (individuals at risk and vaccinated against rabies virus) and a control group (individuals without risk of rabies). Individuals vaccinated against rabies following either “pre-exposure protocol” (PrEP, n = 58) or “post-exposure protocol” (PEP, n = 42) were considered as rabies risk groups and healthy blood donors (n = 30) as the control group. The results showed higher Borrelia seroprevalence in PrEP (17.2%; 10/58) and PEP (19.0%; 8/42) groups compared with the control group (6.67%; 2/30). Furthermore, odds ratio (OR) analysis showed that risk of rabies (in either the PrEP (OR = 2.91) or PEP (OR = 3.29) groups) is associated with increased odds of being seropositive to Borrelia. However, the difference in Borrelia seroprevalence between groups was not statistically significant (Chi-square (χ²) test p 〉 0.05). The shared odds of LB and rabies exposure found in this study suggest that, in countries where both diseases occur, the common citizen can be at risk of both diseases when in a risky habitat. These findings are important to guide physicians in targeting high-risk groups, and diagnose LB, and to guide decision-makers in targeting control and prevention measures for both infections in risk areas.
    Type of Medium: Online Resource
    ISSN: 2076-0817
    URL: Article
    DOI: 10.3390/pathogens10040399
    Language: English
    Publisher: MDPI AG
    Publication Date: 2021
    detail.hit.zdb_id: 2695572-6
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  • 6
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    Online Resource
    Visual, instrumental, mycological and mycotoxicological characterization of wheat inoculated with and protected against Alternaria spp.
    National Library of Serbia ; 2016
    In:  Chemical Industry Vol. 70, No. 3 ( 2016), p. 257-264
    Details
    In: Chemical Industry, National Library of Serbia, Vol. 70, No. 3 ( 2016), p. 257-264
    Abstract: The aim of this work was to characterize visual properties, instrumentally measured colour properties, field fungi presence and Alternaria toxins levels in wheat samples grown under conditions aimed at inhibition and stimulation of wheat infection with fungi from the Alternaria genus. Experiment was carried out on the wheat treated by fungicide and wheat inoculated by Alternaria spp., while non treated wheat was used as a control. Statistically significant difference was observed between all three treatments using visual scale. Protected wheat samples were significantly different from other samples in terms of all measured colour parameters while inoculated and control wheat samples were significantly different in terms of lightness and dominant wavelength. Identification of field fungi in the all examined wheat samples showed that the dominant mycotoxigenic fungus was Alternaria spp., followed by Fusarium spp. The content of Alternaria toxins in samples of wheat hulls and dehulled kernels point out at higher concentrations of Alternaria toxins in hulls than in dehulled kernels.
    Type of Medium: Online Resource
    ISSN: 0367-598X , 2217-7426
    URL: Article
    DOI: 10.2298/HEMIND141114031J
    Language: English
    Publisher: National Library of Serbia
    Publication Date: 2016
    detail.hit.zdb_id: 2928461-2
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  • 7
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    Online Resource
    Sensitivity of Cercospora beticola Isolates to Azoxystrobin
    Walter de Gruyter GmbH ; 2020
    In:  Contemporary Agriculture Vol. 69, No. 1-2 ( 2020-06-01), p. 1-4
    Details
    In: Contemporary Agriculture, Walter de Gruyter GmbH, Vol. 69, No. 1-2 ( 2020-06-01), p. 1-4
    Abstract: Sugar beet leaf spot, caused by the air-borne fungus Cercospora beticola Sacc., leads to a decrease in sugar beet leaf mass and the consequent regrowth of leaves based on exploiting the sugar reserves stored in the plant’s roots, thus ultimately resulting in lower yields and sugar contents of sugar beets. Azoxystrobin belongs to the group of QoI fungicides, which inhibit mitochondrial respiration by blocking cytochrome c reductase. The QoI fungicides are characterized by a very high risk of resistance interfering with their biological activity. For the purpose of testing the azoxystrobin sensitivity of the Cercospora beticola population found at the site of Rimski Šančevi, a collection of 84 isolates was assembled and tested for sensitivity to azoxystrobin by measuring the mycelial growth on fungicide-amended media with the addition of SHAM. The results obtained indicate that none of the isolates tested exhibited complete sensitivity to azoxystrobin, 4% were found to have reduced sensitivity, 26% were moderately resistant and 70% were highly resistant. A higher proportion of resistant isolates recorded is associated with the loss of azoxystrobin biological efficacy at the experimental site.
    Type of Medium: Online Resource
    ISSN: 2466-4774
    URL: Article
    DOI: 10.2478/contagri-2020-0001
    Language: English
    Publisher: Walter de Gruyter GmbH
    Publication Date: 2020
    detail.hit.zdb_id: 2676736-3
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  • 8
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    Online Resource
    Penicillium: Species causing blue mold on stored apple fruits
    Centre for Evaluation in Education and Science (CEON/CEES) ; 2022
    In:  Biljni lekar Vol. 50, No. 2 ( 2022), p. 92-100
    Details
    In: Biljni lekar, Centre for Evaluation in Education and Science (CEON/CEES), Vol. 50, No. 2 ( 2022), p. 92-100
    Abstract: Blue mold, caused by the fungal species of genus Penicillium, is one of the most significant post harvest apple diseases. It is estimated that apple blue mold causes multimillion-dollar losses annually. It can affect any apple variety, due to mechanical damage, inadequate protection or storage conditions. Various Penicillium species can cause apple blue mold, but the most significant is Penicillium expansum. Besides economic losses, Penicillium species cause a threat to human health, due to mycotoxin production. To prevent the apple blue mold, the application of preventive measures is necessary, such as careful handling of fruits during harvest, and maintaining hygienic conditions in the storage. Most of the current preharvest fungicides are applied days before harvest and may not be effective in combating this disease, since Penicillium infections usually occur after the harvest. Therefore, post harvest fungicide applications remain the most effective chemical way to control blue mold. In addition to chemical protection, efforts are made to provide efficient biological solutions for the control of this disease. The incidence of apple blue mold is significantly reduced since controlled atmosphere storages are in use, but it still remains the most important post harvest disease of apple fruits.
    Type of Medium: Online Resource
    ISSN: 0354-6160
    Uniform Title: Penicillium spp. - prouzrokovači plave truleži uskladištenih plodova jabuke
    URL: Article
    DOI: 10.5937/BiljLek2202092D
    Language: English
    Publisher: Centre for Evaluation in Education and Science (CEON/CEES)
    Publication Date: 2022
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  • 9
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    Online Resource
    Diagnostics of prunus necrotic ringspot virus
    Centre for Evaluation in Education and Science (CEON/CEES) ; 2020
    In:  Biljni lekar Vol. 48, No. 1 ( 2020), p. 67-75
    Details
    In: Biljni lekar, Centre for Evaluation in Education and Science (CEON/CEES), Vol. 48, No. 1 ( 2020), p. 67-75
    Type of Medium: Online Resource
    ISSN: 0354-6160
    Uniform Title: Dijagnostika virusa nekrotične prstenaste pegavosti koštičavog voća
    URL: Article
    DOI: 10.5937/BiljLek2001067A
    Language: English
    Publisher: Centre for Evaluation in Education and Science (CEON/CEES)
    Publication Date: 2020
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  • 10
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    Online Resource
    Podosphaera leucotricha (Ellis et Everhart) Salmon: Powdery mildew of apple
    Centre for Evaluation in Education and Science (CEON/CEES) ; 2022
    In:  Biljni lekar Vol. 50, No. 6 ( 2022), p. 438-445
    Details
    In: Biljni lekar, Centre for Evaluation in Education and Science (CEON/CEES), Vol. 50, No. 6 ( 2022), p. 438-445
    Abstract: Apple powdery mildew, caused by Podosphaera leucotricha, is one of the economically most important apple diseases. It is present in all regions where the apple is grown, and it can cause production loss of up to 50%. The appearance of white mycelium on leaves, flowers and shoots is the typical symptom of this disease. If the disease is not managed properly, fruits are not formed, causing direct yield loss. Furthermore, fruit russet appears on the infected fruit, resulting in decreased market value. Disease intensity mostly depends on ecological factors and the susceptibility of the apple variety. Nowadays, significant efforts are being made in order to develop resistant apple varieties, but this goal is yet to be achieved. Management of apple powdery mildew in commercial production is based on chemical control measures, but due to health and environmental concerns, as well as the development of fungicide-resistant populations of the pathogen, it is necessary to find effective biological control methods as an alternative to chemicals.
    Type of Medium: Online Resource
    ISSN: 0354-6160
    Uniform Title: Podosphaera leucotricha (Ellis et Everhart) Salmon - prouzrokovač pepelnice jabuke
    URL: Article
    DOI: 10.5937/BiljLek2206438D
    Language: English
    Publisher: Centre for Evaluation in Education and Science (CEON/CEES)
    Publication Date: 2022
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